دورية أكاديمية
Development of microsatellite markers for Myracrodruon urundeuva (F.F. & M.F. Allemão), a highly endangered species from tropical forest based on next-generation sequencing.
| العنوان: | Development of microsatellite markers for Myracrodruon urundeuva (F.F. & M.F. Allemão), a highly endangered species from tropical forest based on next-generation sequencing. |
|---|---|
| المؤلفون: | Souza DCL; Faculty of Agronomy, UNESP - Univ Estadual Paulista, C.P. 237, CEP 18610-307, Botucatu, SP, Brazil., Rossini BC; Biotechnology Institute (IBTEC), UNESP - Univ Estadual Paulista, CEP 18607-440, Botucatu, SP, Brazil. bu_rossini@yahoo.com.br.; Department of Genetics, UNESP - Univ Estadual Paulista, CEP 18618-689, Botucatu, SP, Brazil. bu_rossini@yahoo.com.br., de Souza FB; Faculty of Engineer, UNESP - Univ Estadual Paulista, CEP 15385-000, Ilha Solteira, SP, Brazil., Sebbenn AM; São Paulo Forestry Institute, C.P. 1322, CEP 01059-970, São Paulo, SP, Brazil., Marino CL; Biotechnology Institute (IBTEC), UNESP - Univ Estadual Paulista, CEP 18607-440, Botucatu, SP, Brazil.; Department of Genetics, UNESP - Univ Estadual Paulista, CEP 18618-689, Botucatu, SP, Brazil., de Moraes MLT; Faculty of Agronomy, UNESP - Univ Estadual Paulista, C.P. 237, CEP 18610-307, Botucatu, SP, Brazil.; Faculty of Engineer, UNESP - Univ Estadual Paulista, CEP 15385-000, Ilha Solteira, SP, Brazil. |
| المصدر: | Molecular biology reports [Mol Biol Rep] 2018 Feb; Vol. 45 (1), pp. 71-75. Date of Electronic Publication: 2017 Dec 29. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Reidel Country of Publication: Netherlands NLM ID: 0403234 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1573-4978 (Electronic) Linking ISSN: 03014851 NLM ISO Abbreviation: Mol Biol Rep Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Dordrecht, Boston, Reidel. |
| مواضيع طبية MeSH: | Anacardiaceae/*genetics , Microsatellite Repeats/*genetics, Alleles ; Brazil ; Conservation of Natural Resources/methods ; Endangered Species ; Forests ; Gene Frequency/genetics ; Genetic Variation ; Genetics, Population/methods ; Genotype ; Heterozygote ; High-Throughput Nucleotide Sequencing/methods ; Polymorphism, Genetic/genetics ; Sequence Analysis, DNA/methods ; Species Specificity ; Trees/genetics |
| مستخلص: | Myracrodruon urundeuva is a tree species of high economic importance due the strength and durability of its wood. Threatened of extinction in Brazil, it is present only in a few forest remnants, mostly in conservation units. Currently, there is little information on the genetic diversity of natural populations in Brazil and even less information about the genome of this species. Here, new species-specific microsatellite loci were developed based on next-generation sequencing (Illumina). More than 100,000 loci were identified in the run, with di- to hexanucleotides motifs. Of these, 20 loci were selected for validation in 30 individuals, with 15 successfully polymorphic loci detected. The number of alleles ranged among loci from 3 to 16, with an average of 7.73, expected (H |
| References: | PLoS One. 2014 May 16;9(5):e95458. (PMID: 24835431) Mol Ecol. 2008 Jul;17(13):3147-59. (PMID: 18522691) Molecules. 2016 Feb 23;21(3):166. (PMID: 26907245) Mol Ecol Resour. 2008 Jan;8(1):103-6. (PMID: 21585727) Mol Ecol. 2007 Mar;16(5):1099-106. (PMID: 17305863) BMC Bioinformatics. 2008 May 29;9:253. (PMID: 18510760) Nat Biotechnol. 2000 Feb;18(2):233-4. (PMID: 10657137) PLoS One. 2015 Oct 06;10(10):e0139775. (PMID: 26440522) J Hered. 2013 Nov-Dec;104(6):881-5. (PMID: 24052535) |
| معلومات مُعتمدة: | 2013/24503-1 Fundação de Amparo à Pesquisa do Estado de São Paulo |
| فهرسة مساهمة: | Keywords: Aroeira; Conservation genetics; Microsatellite; Population genetics |
| تواريخ الأحداث: | Date Created: 20171231 Date Completed: 20180703 Latest Revision: 20181113 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1007/s11033-017-4142-z |
| PMID: | 29288424 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1573-4978 |
|---|---|
| DOI: | 10.1007/s11033-017-4142-z |