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Cryo-EM structure of a Marseilleviridae virus particle reveals a large internal microassembly.

التفاصيل البيبلوغرافية
العنوان: Cryo-EM structure of a Marseilleviridae virus particle reveals a large internal microassembly.
المؤلفون: Okamoto K; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden. Electronic address: kenta.okamoto@icm.uu.se., Miyazaki N; National Institute for Physiological Sciences (NIPS), Okazaki, Aichi, 444-8585 Japan., Reddy HKN; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden., Hantke MF; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden., Maia FRNC; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden., Larsson DSD; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden., Abergel C; Structural and Genomic Information Laboratory, UMR 7256 (IMM FR 3479) Centre National de la Recherche Scientifique & Aix-Marseille University, Marseille 13288, France., Claverie JM; Structural and Genomic Information Laboratory, UMR 7256 (IMM FR 3479) Centre National de la Recherche Scientifique & Aix-Marseille University, Marseille 13288, France; Assistance Publique des Hôpitaux de Marseille, La Timone, 13005 Marseille, France., Hajdu J; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden; Institute of Physics AS CR, v.v.i., Na Slovance 2, 18221 Prague 8, Czech Republic., Murata K; National Institute for Physiological Sciences (NIPS), Okazaki, Aichi, 444-8585 Japan. Electronic address: kazum@nips.ac.jp., Svenda M; Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-75124 Uppsala, Sweden.
المصدر: Virology [Virology] 2018 Mar; Vol. 516, pp. 239-245.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: United States NLM ID: 0110674 Publication Model: Print Cited Medium: Internet ISSN: 1096-0341 (Electronic) Linking ISSN: 00426822 NLM ISO Abbreviation: Virology Subsets: MEDLINE
أسماء مطبوعة: Original Publication: New York, Academic Press.
مواضيع طبية MeSH: DNA Viruses/*physiology , DNA Viruses/*ultrastructure , Virion/*physiology , Virion/*ultrastructure, Capsid/metabolism ; Capsid/ultrastructure ; Cryoelectron Microscopy ; DNA Viruses/genetics ; Genome, Viral ; Viral Proteins/genetics ; Viral Proteins/metabolism ; Virion/genetics ; Virus Assembly
مستخلص: Nucleocytoplasmic large DNA viruses (NCLDVs) blur the line between viruses and cells. Melbournevirus (MelV, family Marseilleviridae) belongs to a new family of NCLDVs. Here we present an electron cryo-microscopy structure of the MelV particle, with the large triangulation number T = 309 constructed by 3080 pseudo-hexagonal capsomers. The most distinct feature of the particle is a large and dense body (LDB) consistently found inside all particles. Electron cryo-tomography of 147 particles shows that the LDB is preferentially located in proximity to the probable lipid bilayer. The LDB is 30 nm in size and its density matches that of a genome/protein complex. The observed LDB reinforces the structural complexity of MelV, setting it apart from other NCLDVs.
(Copyright © 2018 Elsevier Inc. All rights reserved.)
فهرسة مساهمة: Keywords: Amoeba; Capsid; Cryo-electron microscopy; Marseilleviridae; Melbournevirus; NCLDV; Protein complex; Structure; Tomography; Virus
المشرفين على المادة: 0 (Viral Proteins)
تواريخ الأحداث: Date Created: 20180207 Date Completed: 20180601 Latest Revision: 20180601
رمز التحديث: 20240829
DOI: 10.1016/j.virol.2018.01.021
PMID: 29407382
قاعدة البيانات: MEDLINE
الوصف
تدمد:1096-0341
DOI:10.1016/j.virol.2018.01.021