دورية أكاديمية
Extraction optimization and UHPLC method development for determination of the 20-hydroxyecdysone in Sida tuberculata leaves.
| العنوان: | Extraction optimization and UHPLC method development for determination of the 20-hydroxyecdysone in Sida tuberculata leaves. |
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| المؤلفون: | da Rosa HS; Programa de Pós-graduação em Bioquímica, Universidade Federal do Pampa, Uruguaiana, RS, Brazil. Electronic address: hemerdarosa@gmail.com., Koetz M; Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil., Santos MC; Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil., Jandrey EHF; Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil., Folmer V; Programa de Pós-graduação em Bioquímica, Universidade Federal do Pampa, Uruguaiana, RS, Brazil., Henriques AT; Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil., Mendez ASL; Programa de Pós-graduação em Ciências Farmacêuticas, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil; Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil. |
| المصدر: | Steroids [Steroids] 2018 Apr; Vol. 132, pp. 33-39. Date of Electronic Publication: 2018 Feb 03. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: United States NLM ID: 0404536 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1878-5867 (Electronic) Linking ISSN: 0039128X NLM ISO Abbreviation: Steroids Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: New York Ny : Elsevier Original Publication: San Francisco. |
| مواضيع طبية MeSH: | Chromatography, High Pressure Liquid/*methods , Ecdysterone/*analysis , Ecdysterone/*isolation & purification , Malvaceae/*chemistry , Plant Leaves/*chemistry, Ecdysterone/chemistry ; Limit of Detection ; Linear Models ; Reproducibility of Results |
| مستخلص: | Sida tuberculata (ST) is a Malvaceae species widely distributed in Southern Brazil. In traditional medicine, ST has been employed as hypoglycemic, hypocholesterolemic, anti-inflammatory and antimicrobial. Additionally, this species is chemically characterized by flavonoids, alkaloids and phytoecdysteroids mainly. The present work aimed to optimize the extractive technique and to validate an UHPLC method for the determination of 20-hydroxyecdsone (20HE) in the ST leaves. Box-Behnken Design (BBD) was used in method optimization. The extractive methods tested were: static and dynamic maceration, ultrasound, ultra-turrax and reflux. In the Box-Behnken three parameters were evaluated in three levels (-1, 0, +1), particle size, time and plant:solvent ratio. In validation method, the parameters of selectivity, specificity, linearity, limits of detection and quantification (LOD, LOQ), precision, accuracy and robustness were evaluated. The results indicate static maceration as better technique to obtain 20HE peak area in ST extract. The optimal extraction from surface response methodology was achieved with the parameters granulometry of 710 nm, 9 days of maceration and plant:solvent ratio 1:54 (w/v). The UHPLC-PDA analytical developed method showed full viability of performance, proving to be selective, linear, precise, accurate and robust for 20HE detection in ST leaves. The average content of 20HE was 0.56% per dry extract. Thus, the optimization of extractive method in ST leaves increased the concentration of 20HE in crude extract, and a reliable method was successfully developed according to validation requirements and in agreement with current legislation. (Copyright © 2018 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: 20HE; Chromatography; Extract; Phytoecdysteroid; Sida; Validation |
| المشرفين على المادة: | 5289-74-7 (Ecdysterone) |
| تواريخ الأحداث: | Date Created: 20180207 Date Completed: 20181022 Latest Revision: 20181022 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.steroids.2018.01.014 |
| PMID: | 29408449 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1878-5867 |
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| DOI: | 10.1016/j.steroids.2018.01.014 |