دورية أكاديمية
Relationship of antioxidant system and reactive oxygen species with clinical semen parameters in infertile men.
| العنوان: | Relationship of antioxidant system and reactive oxygen species with clinical semen parameters in infertile men. |
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| المؤلفون: | Tomar G; Department of Obstetrics and Gynaecology, Index Medical College Hospital and Research Center, Indore, Madhya Pradesh, India., Joshi T; Department of Obstetrics and Gynaecology, Index Medical College Hospital and Research Center, Indore, Madhya Pradesh, India., Varghes A; Chief Embryologist, CRAFT Hospital and Research Centre, Chennai, Tamil Nadu, India., Sasidharan S; Department of Obstetrics and Gynaecology, Index Medical College Hospital and Research Center, Indore, Madhya Pradesh, India., Kural MR; Department of Obstetrics and Gynaecology, Index Medical College Hospital and Research Center, Indore, Madhya Pradesh, India. |
| المصدر: | Journal of family medicine and primary care [J Family Med Prim Care] 2017 Jul-Sep; Vol. 6 (3), pp. 574-577. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Medknow Country of Publication: India NLM ID: 101610082 Publication Model: Print Cited Medium: Print ISSN: 2249-4863 (Print) Linking ISSN: 22494863 NLM ISO Abbreviation: J Family Med Prim Care Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Mumbai : Medknow |
| مستخلص: | Aims and Objectives: To determine the correlation of antioxidant system and reactive oxygen species with clinical parameters in infertile semen samples. Materials and Methods: Semen sample of fifty infertile men were divided into three groups: (1) Group I - Normospermic (count >15 million/ml), Group II - Asthenospermic (motility <32%), and Group III - Oligospermic (counts <15 million/ml) subjects based on the sperm count and sperm motility. The samples were also divided into two groups: (1) Group IV with semen pH >7.2 (25 samples) and Group V - Semen pH <7.2 (25 samples). The grouping was based on the WHO guideline for semen analysis (12). The semen antioxidant parameters like glucose-6-phosphate dehydrogenase (G-6-PDH) (spectrophotometric method Kornberg and Horecker, 1955). Catalase (Maehly and Chance 1954), glutathione peroxidase (GPX) (Rotruck method), glutathione (GSH) (dithiobisnitro-benzoate method), superoxide dismutase (SOD) (direct method), and malondialdehyde (MDA) (thiobarbituric acid reactive substances assay kit method) were investigated. Mann-Whitney U-test was applied to compare the findings. Results: Of fifty semen samples there were 12 normospermic (sperm concentration ≥15 × 10 6 /ml of ejaculates), 24 asthenospermic (sperm motility ≤32%), and 14 oligospermic (sperm concentration ≤15 × 10 6 /ml of ejaculates) subjects. Results suggested that all asthenospermic males were found to have reduced motility and viability when compared with normospermic and oligospermic subjects. Activity of antioxidant parameters such as G-6-PDH, GPX, GSH, and SOD was decreased in case of asthenospermic subjects. The concentration of MDA was increased significantly ( P < 0.001) in semen of asthenospermic subjects compared to normospermic and oligospermic subjects. Conclusion: The current study concludes that there is a significant relationship of ROS and semen parameters. Further studies will be needed in such subjects regarding role of effectiveness of dietary antioxidants in improving semen qualities. Competing Interests: There are no conflicts of interest. |
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| فهرسة مساهمة: | Keywords: Antioxidant system; infertile; reactive oxygen species; semen parameters |
| تواريخ الأحداث: | Date Created: 20180209 Latest Revision: 20220311 |
| رمز التحديث: | 20240829 |
| مُعرف محوري في PubMed: | PMC5787958 |
| DOI: | 10.4103/2249-4863.222051 |
| PMID: | 29417011 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2249-4863 |
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| DOI: | 10.4103/2249-4863.222051 |