دورية أكاديمية
A Functional Link between RNA Replication and Virion Assembly in the Potyvirus Plum Pox Virus .
| العنوان: | A Functional Link between RNA Replication and Virion Assembly in the Potyvirus Plum Pox Virus . |
|---|---|
| المؤلفون: | Gallo A; Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Madrid, Spain., Valli A; Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Madrid, Spain avalli@cnb.csic.es jagarcia@cnb.csic.es., Calvo M; Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Madrid, Spain., García JA; Centro Nacional de Biotecnología (CNB-CSIC), Campus Universidad Autónoma de Madrid, Madrid, Spain avalli@cnb.csic.es jagarcia@cnb.csic.es. |
| المصدر: | Journal of virology [J Virol] 2018 Apr 13; Vol. 92 (9). Date of Electronic Publication: 2018 Apr 13 (Print Publication: 2018). |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0113724 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1098-5514 (Electronic) Linking ISSN: 0022538X NLM ISO Abbreviation: J Virol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Washington Dc : American Society For Microbiology Original Publication: Baltimore, American Society for Microbiology. |
| مواضيع طبية MeSH: | Capsid Proteins/*genetics , Plum Pox Virus/*genetics , RNA, Viral/*genetics , Virus Assembly/*genetics, Amino Acid Sequence/genetics ; Base Sequence ; Genome, Viral/genetics ; Plant Diseases/virology ; Plant Leaves/virology ; Plasmids/genetics ; Nicotiana/virology ; Virion/genetics ; Virion/metabolism |
| مستخلص: | Accurate assembly of viral particles in the potyvirus Plum pox virus (PPV) has been shown to depend on the contribution of the multifunctional viral protein HCPro. In this study, we show that other viral factors, in addition to the capsid protein (CP) and HCPro, are necessary for the formation of stable PPV virions. The CP produced in Nicotiana benthamiana leaves from a subviral RNA termed LONG, which expresses a truncated polyprotein that lacks P1 and HCPro, together with HCPro supplied in trans , was assembled into virus-like particles and remained stable after in vitro incubation. In contrast, deletions in multiple regions of the LONG coding sequence prevented the CP stabilization mediated by HCPro. In particular, we demonstrated that the first 178 amino acids of P3, but not a specific nucleotide sequence coding for them, are required for CP stability and proper assembly of PPV particles. Using a sequential coagroinfiltration assay, we observed that the subviral LONG RNA replicates and locally spreads in N. benthamiana leaves expressing an RNA silencing suppressor. The analysis of the effect of both point and deletion mutations affecting RNA replication in LONG and full-length PPV demonstrated that this process is essential for the assembly of stable viral particles. Interestingly, in spite of this requirement, the CP produced by a nonreplicating viral RNA can be stably assembled into virions as long as it is coexpressed with a replication-proficient RNA. Altogether, these results highlight the importance of coupling encapsidation to other viral processes to secure a successful infection. IMPORTANCE Viruses of the family Potyviridae are among the most dangerous threats for basically every important crop, and such socioeconomical relevance has made them a subject of many research studies. In spite of this, very little is currently known about proteins and processes controlling viral genome encapsidation by the coat protein. In the case of Plum pox virus (genus Potyvirus ), for instance, we have previously shown that the multitasking viral factor HCPro plays a role in the production of stable virions. Here, by using this potyvirus as a model, we move further to show that additional factors are also necessary for the efficient production of potyviral particles. More importantly, a comprehensive screening for such factors led us to the identification of a functional link between virus replication and packaging, unraveling a previously unknown connection of these two key events of the potyviral infection cycle. (Copyright © 2018 American Society for Microbiology.) |
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| فهرسة مساهمة: | Keywords: Potyviridae; capsid protein; sharka; viral replication; virion assembly |
| المشرفين على المادة: | 0 (Capsid Proteins) 0 (RNA, Viral) |
| تواريخ الأحداث: | Date Created: 20180216 Date Completed: 20180517 Latest Revision: 20231213 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC5899180 |
| DOI: | 10.1128/JVI.02179-17 |
| PMID: | 29444942 |
| قاعدة البيانات: | MEDLINE |
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