دورية أكاديمية
أعرض في EDS

Type IV Pili Promote Clostridium difficile Adherence and Persistence in a Mouse Model of Infection.

التفاصيل البيبلوغرافية
العنوان: Type IV Pili Promote Clostridium difficile Adherence and Persistence in a Mouse Model of Infection.
المؤلفون: McKee RW; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA., Aleksanyan N; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA., Garrett EM; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA., Tamayo R; Department of Microbiology and Immunology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA Rita_tamayo@med.unc.edu.
المصدر: Infection and immunity [Infect Immun] 2018 Apr 23; Vol. 86 (5). Date of Electronic Publication: 2018 Apr 23 (Print Publication: 2018).
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: American Society For Microbiology Country of Publication: United States NLM ID: 0246127 Publication Model: Electronic-Print Cited Medium: Internet ISSN: 1098-5522 (Electronic) Linking ISSN: 00199567 NLM ISO Abbreviation: Infect Immun Subsets: MEDLINE
أسماء مطبوعة: Publication: Washington, DC : American Society For Microbiology
Original Publication: [Bethesda, Md.] American Society for Microbiology.
مواضيع طبية MeSH: Immune Adherence Reaction*, Adhesins, Bacterial/*immunology , Clostridioides difficile/*immunology , Clostridioides difficile/*pathogenicity , Clostridium Infections/*immunology , Clostridium Infections/*pathology , Virulence Factors/*immunology, Animals ; Mice ; Models, Animal
مستخلص: Cyclic diguanylate (c-di-GMP) is a second messenger that regulates the transition from motile to sessile lifestyles in numerous bacteria and controls virulence factor production in a variety of pathogens. In Clostridium difficile , c-di-GMP negatively regulates flagellum biosynthesis and swimming motility and promotes the production of type IV pili (TFP), biofilm formation, and surface motility in vitro Flagella have been identified as colonization factors in C. difficile , but the role of TFP in adherence to host cells and in colonization of the mammalian gut is unknown. Here we show that c-di-GMP promotes adherence to epithelial cells in vitro , which can be partly attributed to the loss of flagella. Using TFP-null mutants, we demonstrate that adherence to epithelial cells is partially mediated by TFP and that this TFP-mediated adherence requires c-di-GMP regulation. In a mouse model of colonization, the TFP-null mutants initially colonized the intestine as well as the parental strain but were cleared more quickly. Moreover, compared to the parent strain, C. difficile strains lacking TFP were particularly deficient in association with the cecal mucosa. Together these data indicate that TFP and their positive regulation by c-di-GMP promote attachment of C. difficile to the intestinal epithelium and contribute to persistence of C. difficile in the host intestine.
(Copyright © 2018 American Society for Microbiology.)
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معلومات مُعتمدة: R01 AI107029 United States AI NIAID NIH HHS
فهرسة مساهمة: Keywords: Clostridioides; Clostridioides difficile; TcdR; adhesin; c-di-GMP; colonization; riboswitch; type IV pili
المشرفين على المادة: 0 (Adhesins, Bacterial)
0 (Virulence Factors)
تواريخ الأحداث: Date Created: 20180228 Date Completed: 20181217 Latest Revision: 20201209
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC5913833
DOI: 10.1128/IAI.00943-17
PMID: 29483294
قاعدة البيانات: MEDLINE
الوصف
تدمد:1098-5522
DOI:10.1128/IAI.00943-17