دورية أكاديمية
Rheum palmatum root extract inhibits hepatocellular carcinoma in rats treated with diethylnitrosamine.
| العنوان: | Rheum palmatum root extract inhibits hepatocellular carcinoma in rats treated with diethylnitrosamine. |
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| المؤلفون: | El-Saied MA; Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Menoufia Province, Egypt., Sobeh M; Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg, Germany., Abdo W; Pathology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh, Egypt., Badr OM; Animal Biotechnology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Menoufia Province, Egypt., Youssif LT; Molecular Diagnostics Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Menoufia Province, Egypt., Elsayed IH; Molecular Biology Department, Genetic Engineering and Biotechnology Research Institute, University of Sadat City, Sadat City, Menoufia Province, Egypt., Osman SM; Pharmacognosy Department, Faculty of Pharmacy, October 06 University, Cairo, Egypt., Wink M; Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg, Germany. |
| المصدر: | The Journal of pharmacy and pharmacology [J Pharm Pharmacol] 2018 Jun; Vol. 70 (6), pp. 821-829. Date of Electronic Publication: 2018 Mar 09. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Oxford University Press Country of Publication: England NLM ID: 0376363 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2042-7158 (Electronic) Linking ISSN: 00223573 NLM ISO Abbreviation: J Pharm Pharmacol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2021- : [Oxford] : Oxford University Press Original Publication: London : Pharmaceutical Society of Great Britain |
| مواضيع طبية MeSH: | Liver Neoplasms, Experimental/*drug therapy , Phytotherapy/*methods , Plant Extracts/*therapeutic use , Plant Roots/*chemistry , Rheum/*chemistry, Alanine Transaminase/blood ; Alkaline Phosphatase/blood ; Animals ; Aspartate Aminotransferases/blood ; DNA Fragmentation/drug effects ; Diethylnitrosamine ; Glutathione S-Transferase pi/metabolism ; Liver Neoplasms, Experimental/blood ; Liver Neoplasms, Experimental/chemically induced ; Male ; Plant Extracts/pharmacology ; Rats ; Serum Albumin/metabolism ; Serum Globulins/metabolism ; alpha-Fetoproteins/metabolism ; gamma-Glutamyltransferase/blood |
| مستخلص: | Objectives: The aim of this study was to investigate the potential anticancer properties of a methanol extract of Rheum palmatum roots against diethylnitrosamine (DENA)-induced hepatocellular carcinoma (HCC) in rats and to characterize its phytoconstituents. Methods: HPLC-PDA-MS/MS was used to profile the secondary metabolites in R. palmatum root extract. HCC was induced using diethylnitrosamine (DENA). The activity of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and gamma-glutamyl transferase (GGT), alpha-fetoprotein (AFP), total proteins, serum albumin and serum globulin was determined. DNA fragmentation and histopathological examination and GST-P immunostaining were also studied. Key Findings: LC-MS/MS analysis identified 16 compounds belonging to anthraquinones, flavonoids and tannins. The root extract significantly reduced the elevated liver enzymes ALT and AST and increased total proteins, albumin and globulin in HCC-rats. Also, the tumour markers AFP and GGT levels were significantly reduced in HCC-rats treated with the extract. In addition, the extract significantly reduced elevated DNA fragmentation and decreased the numbers and areas of GST-P positive putative foci in HCC-rats. Conclusions: Rheum palmatum is a potential candidate to be explored for the treatment of hepatocellular carcinoma. (© 2018 Royal Pharmaceutical Society.) |
| فهرسة مساهمة: | Keywords: Rheum palmatum; DNA fragmentation; HPLC-MS/MS; diethylnitrosamine; hepatocellular carcinoma |
| المشرفين على المادة: | 0 (Plant Extracts) 0 (Serum Albumin) 0 (Serum Globulins) 0 (alpha-Fetoproteins) 3IQ78TTX1A (Diethylnitrosamine) EC 2.3.2.2 (gamma-Glutamyltransferase) EC 2.5.1.18 (Glutathione S-Transferase pi) EC 2.5.1.18 (Gstp1 protein, rat) EC 2.6.1.1 (Aspartate Aminotransferases) EC 2.6.1.2 (Alanine Transaminase) EC 3.1.3.1 (Alkaline Phosphatase) |
| تواريخ الأحداث: | Date Created: 20180310 Date Completed: 20181011 Latest Revision: 20181011 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1111/jphp.12899 |
| PMID: | 29520909 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2042-7158 |
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| DOI: | 10.1111/jphp.12899 |