دورية أكاديمية
أعرض في EDS

Neuroprotective effect of paeoniflorin on okadaic acid-induced tau hyperphosphorylation via calpain/Akt/GSK-3β pathway in SH-SY5Y cells.

التفاصيل البيبلوغرافية
العنوان: Neuroprotective effect of paeoniflorin on okadaic acid-induced tau hyperphosphorylation via calpain/Akt/GSK-3β pathway in SH-SY5Y cells.
المؤلفون: Ma XH; Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510405, China., Duan WJ; College of Pharmacy, Jinan University, Guangzhou 510080, China., Mo YS; Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510405, China., Chen JL; Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510405, China., Li S; Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510405, China., Zhao W; Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510405, China., Yang L; Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510405, China., Mi SQ; Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510405, China., Mao XL; Department of Pharmacology, College of Pharmaceutical Sciences, Soochow University, Suzhou 215123, China., Wang H; Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510405, China. Electronic address: zyjw@gzucm.edu.cn., Wang Q; Institute of Clinical Pharmacology, Guangzhou University of Chinese Medicine, Guangzhou 510405, China. Electronic address: wangqi@gzucm.edu.cn.
المصدر: Brain research [Brain Res] 2018 Jul 01; Vol. 1690, pp. 1-11. Date of Electronic Publication: 2018 Mar 27.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier/North-Holland Biomedical Press Country of Publication: Netherlands NLM ID: 0045503 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1872-6240 (Electronic) Linking ISSN: 00068993 NLM ISO Abbreviation: Brain Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam Elsevier/North-Holland Biomedical Press.
مواضيع طبية MeSH: Alzheimer Disease/*diagnostic imaging , Glucosides/*pharmacology , Monoterpenes/*pharmacology , Neurons/*drug effects , Neuroprotective Agents/*pharmacology , tau Proteins/*metabolism, Alzheimer Disease/metabolism ; Autophagy/drug effects ; Autophagy/physiology ; Calpain/metabolism ; Cell Line, Tumor ; Glycogen Synthase Kinase 3 beta/metabolism ; Humans ; Microtubules/drug effects ; Microtubules/metabolism ; Neurons/metabolism ; Neurons/pathology ; Okadaic Acid/toxicity ; Phosphorylation ; Proto-Oncogene Proteins c-akt/metabolism
مستخلص: Abnormal phosphorylation of tau, one of the most common symptoms of dementia, has become increasingly important in the study of the etiology and development of Alzheimer's disease. Paeoniflorin, the main bioactive component of herbaceous peony, is a monoterpene glycoside, which has been reported to exert beneficial effects on neurodegenerative disease. However, the effect of paeoniflorin on tauopathies remains ambiguous. SH-SY5Y cells were treated with okadaic acid (OA) for 8 h to induce tau phosphorylation and no cell death was observed. Optical microscopy results showed that paeoniflorin ameliorated okadaic acid induced morphological changes, including cell swelling and synapsis shortening. Western blotting data illustrated that paeoniflorin reversed okadaic acid induced tau hyperphosphorylation, which was enhanced by inhibiting the activities of calpain, Akt and GSK-3β. Transmission electron microscopy results showed that paeoniflorin alone can reduce the number of autophagosomes and stabilize the microtubule structure. In addition, calpastain and paeoniflorin enhance the effect of paeoniflorin on stabilizing microtubules. In addition, calpastain markedly enhanced the effect of paeoniflorin on reversing okadaic acid-lowered fluorescence intensity of both MAP-2 and β III-tubulin, two microtubule-associated proteins. This study shows that paeoniflorin protected SH-SY5Y cells against okadaic acid assault by interfering with the calpain/Akt/GSK-3β-related pathways, in which autophagy might be involved. Besides, paeoniflorin is found to relieve the stress response of the microtubule structure system caused by okadaic acid treatment. The results presented in this study suggest that paeoniflorin potentially plays an important role in tauopathies.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Alzheimer’s disease; Autophagy; Paeoniflorin; Tau; μ-calpain
المشرفين على المادة: 0 (Glucosides)
0 (MAPT protein, human)
0 (Monoterpenes)
0 (Neuroprotective Agents)
0 (tau Proteins)
1W21G5Q4N2 (Okadaic Acid)
21AIQ4EV64 (peoniflorin)
EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta)
EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
EC 3.4.22.- (Calpain)
تواريخ الأحداث: Date Created: 20180330 Date Completed: 20190411 Latest Revision: 20190411
رمز التحديث: 20231215
DOI: 10.1016/j.brainres.2018.03.022
PMID: 29596798
قاعدة البيانات: MEDLINE
الوصف
تدمد:1872-6240
DOI:10.1016/j.brainres.2018.03.022