دورية أكاديمية
Characterizing Intact Macromolecular Complexes Using Native Mass Spectrometry.
| العنوان: | Characterizing Intact Macromolecular Complexes Using Native Mass Spectrometry. |
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| المؤلفون: | Boeri Erba E; Institut de Biologie Structurale (IBS), Université de Grenoble Alpes, CEA, CNRS, Grenoble, France. elisabetta.boeri-erba@ibs.fr., Signor L; Institut de Biologie Structurale (IBS), Université de Grenoble Alpes, CEA, CNRS, Grenoble, France., Oliva MF; Institut de Biologie Structurale (IBS), Université de Grenoble Alpes, CEA, CNRS, Grenoble, France., Hans F; Institut de Biologie Structurale (IBS), Université de Grenoble Alpes, CEA, CNRS, Grenoble, France., Petosa C; Institut de Biologie Structurale (IBS), Université de Grenoble Alpes, CEA, CNRS, Grenoble, France. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1764, pp. 133-151. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Histones/*metabolism , Macromolecular Substances/*metabolism , Mass Spectrometry/*methods , Ribonucleases/*metabolism , Saccharomyces cerevisiae Proteins/*metabolism, Histones/chemistry ; Macromolecular Substances/chemistry ; Ribonucleases/chemistry ; Saccharomyces cerevisiae Proteins/chemistry |
| مستخلص: | Native mass spectrometry (MS) enables the characterization of macromolecular assemblies with high sensitivity. It can reveal the stoichiometry of subunits as well as their two-dimensional interaction network and provide information regarding the dynamic behavior of macromolecular complexes. Here, we describe the workflow to perform native MS experiments. In addition, we illustrate the quality control analysis of proteins using MS in denaturing conditions. |
| التعليقات: | Erratum in: Methods Mol Biol. 2018;1764:E1. (PMID: 30043152) |
| فهرسة مساهمة: | Keywords: Macromolecular assemblies; Native mass spectrometry; Stoichiometry; Two-dimensional map of interactions |
| المشرفين على المادة: | 0 (Histones) 0 (Macromolecular Substances) 0 (Saccharomyces cerevisiae Proteins) EC 3.1.- (Ribonucleases) EC 3.1.13.4 (POP2 protein, S cerevisiae) |
| تواريخ الأحداث: | Date Created: 20180402 Date Completed: 20190222 Latest Revision: 20190222 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/978-1-4939-7759-8_9 |
| PMID: | 29605913 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-4939-7759-8_9 |