دورية أكاديمية
Use of fatty acids in the chemotaxonomy of the family Selenastraceae (Sphaeropleales, Chlorophyceae).
| العنوان: | Use of fatty acids in the chemotaxonomy of the family Selenastraceae (Sphaeropleales, Chlorophyceae). |
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| المؤلفون: | Mori CC; Phycology Laboratory, Department of Botany, Federal University of São Carlos, Washington Luís Highway SP 310, 235 km, São Carlos, São Paulo, 13565-905, Brazil. Electronic address: cilenemori@gmail.com., Bagatini IL; Phycology Laboratory, Department of Botany, Federal University of São Carlos, Washington Luís Highway SP 310, 235 km, São Carlos, São Paulo, 13565-905, Brazil., Garcia da Silva T; Phycology Laboratory, Department of Botany, Federal University of São Carlos, Washington Luís Highway SP 310, 235 km, São Carlos, São Paulo, 13565-905, Brazil., Parrish CC; Department of Ocean Sciences, Memorial University of Newfoundland, Ocean Sciences Centre, Marine Lab Road, St. John's, Newfoundland A1C 5S7, Canada., Henriques Vieira AA; Phycology Laboratory, Department of Botany, Federal University of São Carlos, Washington Luís Highway SP 310, 235 km, São Carlos, São Paulo, 13565-905, Brazil. |
| المصدر: | Phytochemistry [Phytochemistry] 2018 Jul; Vol. 151, pp. 9-16. Date of Electronic Publication: 2018 Apr 06. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 0151434 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-3700 (Electronic) Linking ISSN: 00319422 NLM ISO Abbreviation: Phytochemistry Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2003- : London : Elsevier Original Publication: 1961-2003: Oxford : Pergamon Press. |
| مواضيع طبية MeSH: | Fatty Acids/*analysis , Microalgae/*chemistry |
| مستخلص: | The family Selenastraceae includes many species of freshwater green microalgae with morphological characteristics that are so subtly different that it is difficult to discriminate species within it. Therefore, the use of the diacritical characteristics of traditional morphological taxonomy may be ineffective at differentiating among many species of the family. Chemotaxonomy based on fatty acid methyl ester (FAME) can help resolve uncertainties not completely addressed by other approaches, such as molecular studies of some species within the Selenastraceae. Here, we first tested three techniques for the analysis of microalgal FAME to choose the one that would provide the best profiles for chemotaxonomy: 1) direct transesterification (DT) of the biomass followed by gas chromatography coupled to mass spectrometry analysis (GC-MS); 2) extraction using chloroform and methanol, followed by transesterification (T) and then analysis by gas chromatography/flame ionization detector (GC-FID); and 3) extraction with chloroform and methanol and then separation into lipid classes using thin-layer chromatography (TLC) using the Iatroscan-Chromarod system. The tests were conducted on 12 strains (11 species) of Selenastraceae and one outgroup strain. The fatty acid profiles produced by the DT-GC-MS technique yielded the best results for the chemotaxonomy of the Selenastraceae species using 12 FAME. The proportion of the variance in the fatty acid profiles obtained with DT-GC-MS analysis explained by species was 85%, whereas the differences explained by strains was 92%. Therefore, DT-GC-MS was used to analyze other microalgae strains, totaling 15 species of 8 genera of green coccoid microalgae, including the recently described Curvastrum. The results with all strains showed that fatty acid profiles obtained by DT-GC-MS were significantly different (p < 0.001) among strains and among species. The variance in fatty acids profiles explained by separation into strains was 97%, whereas the separation into species explained 93% of the variance. Statistical analyses showed that, for our dataset, the C (Copyright © 2018 Elsevier Ltd. All rights reserved.) |
| التعليقات: | Erratum in: Phytochemistry. 2021 Jul;187:112778. (PMID: 33933826) |
| فهرسة مساهمة: | Keywords: Chemotaxonomy; FAME; Fatty acids; Gas chromatography (GC-FID); Iatroscan (TLC); Mass spectrometry (GC-MS); Selenastraceae |
| المشرفين على المادة: | 0 (Fatty Acids) |
| تواريخ الأحداث: | Date Created: 20180410 Date Completed: 20180626 Latest Revision: 20210502 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.phytochem.2018.03.011 |
| PMID: | 29631105 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1873-3700 |
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| DOI: | 10.1016/j.phytochem.2018.03.011 |