دورية أكاديمية
Prevalence of Mycoplasma genitalium and mutations associated with macrolide and fluoroquinolone resistance in Finland.
| العنوان: | Prevalence of Mycoplasma genitalium and mutations associated with macrolide and fluoroquinolone resistance in Finland. |
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| المؤلفون: | Hokynar K; 1 Department of Virology, University of Helsinki, Helsinki, Finland., Hiltunen-Back E; 2 Department of Dermatology, Helsinki University Central Hospital, Helsinki, Finland., Mannonen L; 3 Virology and Immunology, Helsinki University Hospital, Helsinki, Finland., Puolakkainen M; 1 Department of Virology, University of Helsinki, Helsinki, Finland.; 3 Virology and Immunology, Helsinki University Hospital, Helsinki, Finland. |
| المصدر: | International journal of STD & AIDS [Int J STD AIDS] 2018 Aug; Vol. 29 (9), pp. 904-907. Date of Electronic Publication: 2018 Apr 09. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Sage Country of Publication: England NLM ID: 9007917 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1758-1052 (Electronic) Linking ISSN: 09564624 NLM ISO Abbreviation: Int J STD AIDS Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Dec. 2012- : London : Sage Original Publication: London, U.K. : Royal Society of Medicine Services, c1990- |
| مواضيع طبية MeSH: | Drug Resistance, Bacterial*/genetics, Anti-Bacterial Agents/*therapeutic use , Fluoroquinolones/*therapeutic use , Macrolides/*therapeutic use , Mycoplasma Infections/*drug therapy , Mycoplasma genitalium/*drug effects , Mycoplasma genitalium/*genetics, DNA, Bacterial/genetics ; Finland/epidemiology ; Humans ; Mutation ; Mycoplasma Infections/epidemiology ; Mycoplasma Infections/microbiology ; Mycoplasma genitalium/isolation & purification ; Polymerase Chain Reaction ; Prevalence ; Sequence Analysis, DNA |
| مستخلص: | The aim was to examine the prevalence of Mycoplasma genitalium and to determine the prevalence of mutations leading to resistance to macrolides and fluoroquinolones in a sexually transmitted infection clinic setting in Finland, and as a service evaluation, to validate the performance of a commercial Aptima® Mycoplasma genitalium assay. Urogenital samples were studied for M. genitalium with an automated commercial Aptima® Mycoplasma genitalium assay on the Panther® system (Hologic), and with an in-house real-time polymerase chain reaction (PCR) (mgpB). Positive specimens were further studied for mutations associated with macrolide resistance within the 23S rRNA gene and the known quinolone resistance-determining regions within genes gyrA, gyrB and parC. Altogether 17/303 (5.6%) of samples contained M. genitalium by either test. Two of the samples positive by the Aptima assay were not detected by the in-house PCR assay, although the internal control (beta-globin gene) was amplified. The Aptima assay gave an invalid result for five samples, all of which were negative by the in-house PCR. Mutations resulting in macrolide resistance were detected in 30.8% of M. genitalium-positive specimens. Prevalence of M. genitalium infections in the specimens tested is similar to that in other parts of Europe, 5.6%. The Aptima® Mycoplasma genitalium assay detected slightly more positives than the in-house PCR assay. Mutations resulting in macrolide resistance were common in M. genitalium and detection of these mutations is recommended in diagnostic laboratories to assist in selection of treatment. |
| فهرسة مساهمة: | Keywords: Mycoplasma genitalium; antibiotic; diagnosis; urethritis (bacterial) |
| المشرفين على المادة: | 0 (Anti-Bacterial Agents) 0 (DNA, Bacterial) 0 (Fluoroquinolones) 0 (Macrolides) |
| تواريخ الأحداث: | Date Created: 20180411 Date Completed: 20190326 Latest Revision: 20190326 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1177/0956462418764482 |
| PMID: | 29631509 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1758-1052 |
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| DOI: | 10.1177/0956462418764482 |