دورية أكاديمية
Extracellular vesicles from human saliva promote hemostasis by delivering coagulant tissue factor to activated platelets.
| العنوان: | Extracellular vesicles from human saliva promote hemostasis by delivering coagulant tissue factor to activated platelets. |
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| المؤلفون: | Yu Y; Laboratory of Experimental Clinical Chemistry, Academic Medical Centre (AMC) of the University of Amsterdam, Amsterdam, the Netherlands.; Vesicle Observation Centre, AMC, Amsterdam, the Netherlands., Gool E; Laboratory of Experimental Clinical Chemistry, Academic Medical Centre (AMC) of the University of Amsterdam, Amsterdam, the Netherlands.; Vesicle Observation Centre, AMC, Amsterdam, the Netherlands.; Department of Biomedical Engineering and Physics, AMC, Amsterdam, the Netherlands., Berckmans RJ; Laboratory of Experimental Clinical Chemistry, Academic Medical Centre (AMC) of the University of Amsterdam, Amsterdam, the Netherlands.; Vesicle Observation Centre, AMC, Amsterdam, the Netherlands., Coumans FAW; Vesicle Observation Centre, AMC, Amsterdam, the Netherlands.; Department of Biomedical Engineering and Physics, AMC, Amsterdam, the Netherlands., Barendrecht AD; Department of Clinical Chemistry and Haematology, University Medical Center Utrecht, Utrecht, the Netherlands., Maas C; Department of Clinical Chemistry and Haematology, University Medical Center Utrecht, Utrecht, the Netherlands., van der Wel NN; Department of Medical Biology, Electron Microscopy Centre Amsterdam, AMC, Amsterdam, the Netherlands., Altevogt P; Skin Cancer Unit, German Cancer Research Center, Heidelberg, Germany.; Department of Dermatology, Venereology and Allergology, University Medical Center Mannheim, Ruprecht-Karl University of Heidelberg, Heidelberg, Germany., Sturk A; Laboratory of Experimental Clinical Chemistry, Academic Medical Centre (AMC) of the University of Amsterdam, Amsterdam, the Netherlands.; Vesicle Observation Centre, AMC, Amsterdam, the Netherlands., Nieuwland R; Laboratory of Experimental Clinical Chemistry, Academic Medical Centre (AMC) of the University of Amsterdam, Amsterdam, the Netherlands.; Vesicle Observation Centre, AMC, Amsterdam, the Netherlands. |
| المصدر: | Journal of thrombosis and haemostasis : JTH [J Thromb Haemost] 2018 Jun; Vol. 16 (6), pp. 1153-1163. Date of Electronic Publication: 2018 May 17. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: England NLM ID: 101170508 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1538-7836 (Electronic) Linking ISSN: 15387836 NLM ISO Abbreviation: J Thromb Haemost Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2023- : [New York] : Elsevier Original Publication: Oxford : Blackwell Pub. |
| مواضيع طبية MeSH: | Blood Coagulation* , Platelet Activation*, Blood Platelets/*metabolism , Extracellular Vesicles/*metabolism , Saliva/*metabolism , Thromboplastin/*metabolism, CD24 Antigen/metabolism ; Humans ; Ligands ; P-Selectin/metabolism ; Saliva/cytology ; Signal Transduction |
| مستخلص: | Essentials Human salivary extracellular vesicles (EVs) expose coagulant tissue factor (TF). Salivary EVs expose CD24, a ligand of P-selectin. CD24 and coagulant TF co-localize on salivary EVs. TF + /CD24 + salivary EVs bind to activated platelets and trigger coagulation. Summary: Background Extracellular vesicles (EVs) from human saliva expose coagulant tissue factor (TF). Whether such TF-exposing EVs contribute to hemostasis, however, is unknown. Recently, in a mice model, tumor cell-derived EVs were shown to deliver coagulant TF to activated platelets at a site of vascular injury via interaction between P-selectin glycoprotein ligand-1 (PSGL-1) and P-selectin. Objectives We hypothesized that salivary EVs may deliver coagulant TF to activated platelets via interaction with P-selectin. Methods We investigated the presence of two ligands of P-selectin on salivary EVs, PSGL-1 and CD24. Results Salivary EVs expose CD24 but PSGL-1 was not detected. Immune depletion of CD24-exposing EVs completely abolished the TF-dependent coagulant activity of cell-free saliva, showing that coagulant TF and CD24 co-localize on salivary EVs. In a whole blood perfusion model, salivary EVs accumulated at the surface of activated platelets and promoted fibrin generation, which was abolished by an inhibitory antibody against human CD24. Conclusions A subset of EVs in human saliva expose coagulant TF and CD24, a ligand of P-selectin, suggesting that such EVs may facilitate hemostasis at a site of skin injury where the wound is licked in a reflex action. (© 2018 The Authors. Journal of Thrombosis and Haemostasis published by Wiley Periodicals, Inc. on behalf of International Society on Thrombosis and Haemostasis.) |
| فهرسة مساهمة: | Keywords: P-selectin; coagulation; extracellular vesicles; saliva; tissue factor |
| المشرفين على المادة: | 0 (CD24 Antigen) 0 (CD24 protein, human) 0 (Ligands) 0 (P-Selectin) 0 (SELP protein, human) 9035-58-9 (Thromboplastin) |
| تواريخ الأحداث: | Date Created: 20180417 Date Completed: 20190916 Latest Revision: 20230829 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1111/jth.14023 |
| PMID: | 29658195 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1538-7836 |
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| DOI: | 10.1111/jth.14023 |