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Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi.

التفاصيل البيبلوغرافية
العنوان: Development and worldwide use of non-lethal, and minimal population-level impact, protocols for the isolation of amphibian chytrid fungi.
المؤلفون: Fisher MC; Department of Infectious Disease Epidemiology, School of Public Health, Faculty of Medicine (St Mary's campus), Imperial College London, London, W2 1PG, UK. matthew.fisher@imperial.ac.uk., Ghosh P; Department of Infectious Disease Epidemiology, School of Public Health, Faculty of Medicine (St Mary's campus), Imperial College London, London, W2 1PG, UK.; Unit for Environmental Sciences and Management, Private Bag x6001, North-West University, Potchefstroom, 2520, South Africa., Shelton JMG; Department of Infectious Disease Epidemiology, School of Public Health, Faculty of Medicine (St Mary's campus), Imperial College London, London, W2 1PG, UK., Bates K; Department of Infectious Disease Epidemiology, School of Public Health, Faculty of Medicine (St Mary's campus), Imperial College London, London, W2 1PG, UK., Brookes L; Institute of Zoology, Regent's Park, London, NW1 4RY, UK., Wierzbicki C; Department of Infectious Disease Epidemiology, School of Public Health, Faculty of Medicine (St Mary's campus), Imperial College London, London, W2 1PG, UK., Rosa GM; Institute of Zoology, Regent's Park, London, NW1 4RY, UK.; Centre for Ecology, Evolution and Environmental Changes (CE3C), Faculdade de Ciências da Universidade de Lisboa, Lisboa, Portugal., Farrer RA; Department of Infectious Disease Epidemiology, School of Public Health, Faculty of Medicine (St Mary's campus), Imperial College London, London, W2 1PG, UK., Aanensen DM; Department of Infectious Disease Epidemiology, School of Public Health, Faculty of Medicine (St Mary's campus), Imperial College London, London, W2 1PG, UK.; Centre for Genomic Pathogen Surveillance, Wellcome Genome Campus, Cambridgeshire, UK., Alvarado-Rybak M; Centro de Investigación para la Sustentabilidad, Facultad de Ecología y Recursos Naturales, Universidad Andres Bello, Republica 440, Santiago, Chile., Bataille A; Laboratory of Behavioral and Population Ecology, School of Biological Sciences, Seoul National University, Seoul, 08826, South Korea.; CIRAD, UMR ASTRE, F-34398 Montpellier, France; ASTRE, Univ Montpellier, CIRAD, INRA, Montpellier, France., Berger L; One Health Research Group, College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, Queensland, 4811, Australia., Böll S; Agency for Population Ecology and Nature Conservancy, Gerbrunn, Germany., Bosch J; Museo Nacional de Ciencias Naturales, CSIC c/Jose Gutierrez Abascal 2, 28006, Madrid, Spain., Clare FC; Department of Infectious Disease Epidemiology, School of Public Health, Faculty of Medicine (St Mary's campus), Imperial College London, London, W2 1PG, UK., A Courtois E; Laboratoire Ecologie, évolution, interactions des systèmes amazoniens (LEEISA), Université de Guyane, CNRS, IFREMER, 97300, Cayenne, French Guiana., Crottini A; CIBIO - Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO, Universidade do Porto, 4485-661, Vairão, Portugal., Cunningham AA; Institute of Zoology, Regent's Park, London, NW1 4RY, UK., Doherty-Bone TM; Conservation Programmes, Royal Zoological Society of Scotland, Edinburgh, UK., Gebresenbet F; Department of Integrative Biology, Oklahoma State University, 113 Life Sciences West, Stillwater, OK, 74078, USA., Gower DJ; Life Sciences, The Natural History Museum, London, SW7 5BD, UK., Höglund J; Department of Ecology and Genetics, EBC, Uppsala University, Norbyv. 18D, SE-75236, Uppsala, Sweden., James TY; Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, Michigan, 48109, USA., Jenkinson TS; Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, Michigan, 48109, USA., Kosch TA; Laboratory of Behavioral and Population Ecology, School of Biological Sciences, Seoul National University, Seoul, 08826, South Korea.; One Health Research Group, College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, Queensland, 4811, Australia., Lambertini C; Laboratório de História Natural de Anfíbios Brasileiros (LaHNAB), Departamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, São Paulo, 13083-862, Brazil., Laurila A; Department of Ecology and Genetics, EBC, Uppsala University, Norbyv. 18D, SE-75236, Uppsala, Sweden., Lin CF; Zoology Division, Endemic Species Research Institute, 1 Ming-shen East Road, Jiji, Nantou, 552, Taiwan., Loyau A; Helmholtz Centre for Environmental Research - UFZ, Department of Conservation Biology, Permoserstrasse 15, 04318, Leipzig, Germany.; ECOLAB, Université de Toulouse, CNRS, INPT, UPS, Toulouse, France., Martel A; Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820, Merelbeke, Belgium., Meurling S; Department of Ecology and Genetics, EBC, Uppsala University, Norbyv. 18D, SE-75236, Uppsala, Sweden., Miaud C; PSL Research University, CEFE UMR 5175, CNRS, Université de Montpellier, Université Paul-Valéry Montpellier, EPHE, Biogéographie et Ecologie des vertébrés, Montpellier, France., Minting P; Amphibian and Reptile Conservation (ARC) Trust, 655A Christchurch Road, Boscombe, Bournemouth, Dorset, BH1 4AP, UK., Ndriantsoa S; Durrell Wildlife Conservation Trust, Madagascar Programme, Antananarivo, Madagascar., O'Hanlon SJ; Department of Infectious Disease Epidemiology, School of Public Health, Faculty of Medicine (St Mary's campus), Imperial College London, London, W2 1PG, UK.; Institute of Zoology, Regent's Park, London, NW1 4RY, UK., Pasmans F; Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820, Merelbeke, Belgium., Rakotonanahary T; Durrell Wildlife Conservation Trust, Madagascar Programme, Antananarivo, Madagascar., Rabemananjara FCE; Durrell Wildlife Conservation Trust, Madagascar Programme, Antananarivo, Madagascar.; IUCN SSC Amphibian Specialist Group-Madagascar, 101, Antananarivo, Madagascar., Ribeiro LP; Laboratório de História Natural de Anfíbios Brasileiros (LaHNAB), Departamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, São Paulo, 13083-862, Brazil., Schmeller DS; Helmholtz Centre for Environmental Research - UFZ, Department of Conservation Biology, Permoserstrasse 15, 04318, Leipzig, Germany.; ECOLAB, Université de Toulouse, CNRS, INPT, UPS, Toulouse, France., Schmidt BR; Department of Evolutionary Biology and Environmental Studies, University of Zurich, Winterthurerstrasse 190, 8057, Zurich, Switzerland.; Info Fauna Karch, Université de Neuchâtel, Bellevaux 51, UniMail Bâtiment 6, 2000, Neuchâtel, Switzerland., Skerratt L; One Health Research Group, College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, Queensland, 4811, Australia., Smith F; National Wildlife Management Centre, APHA, Woodchester Park, Gloucestershire, GL10 3UJ, UK., Soto-Azat C; Centro de Investigación para la Sustentabilidad, Facultad de Ecología y Recursos Naturales, Universidad Andres Bello, Republica 440, Santiago, Chile., Tessa G; Non-profit Association Zirichiltaggi - Sardinia Wildlife Conservation, Strada Vicinale Filigheddu 62/C, I-07100, Sassari, Italy., Toledo LF; Laboratório de História Natural de Anfíbios Brasileiros (LaHNAB), Departamento de Biologia Animal, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, São Paulo, 13083-862, Brazil., Valenzuela-Sánchez A; Centro de Investigación para la Sustentabilidad, Facultad de Ecología y Recursos Naturales, Universidad Andres Bello, Republica 440, Santiago, Chile.; ONG Ranita de Darwin, Nataniel Cox 152, Santiago, Chile., Verster R; Unit for Environmental Sciences and Management, Private Bag x6001, North-West University, Potchefstroom, 2520, South Africa., Vörös J; Collection of Amphibians and Reptiles, Department of Zoology, Hungarian Natural History Museum, Budapest, Baross u, 13., 1088, Hungary., Waldman B; Laboratory of Behavioral and Population Ecology, School of Biological Sciences, Seoul National University, Seoul, 08826, South Korea., Webb RJ; One Health Research Group, College of Public Health, Medical and Veterinary Sciences, James Cook University, Townsville, Queensland, 4811, Australia., Weldon C; Unit for Environmental Sciences and Management, Private Bag x6001, North-West University, Potchefstroom, 2520, South Africa., Wombwell E; Institute of Zoology, Regent's Park, London, NW1 4RY, UK., Zamudio KR; Department of Ecology and Evolutionary Biology, Cornell University, Ithaca, New York, 14853, USA., Longcore JE; School of Biology and Ecology, University of Maine, Orono, Maine, 04469, USA., Garner TWJ; Institute of Zoology, Regent's Park, London, NW1 4RY, UK.; Non-profit Association Zirichiltaggi - Sardinia Wildlife Conservation, Strada Vicinale Filigheddu 62/C, I-07100, Sassari, Italy.; Unit for Environmental Sciences and Management, Private Bag x6001, North-West University, Potchefstroom, 2520, South Africa.
المصدر: Scientific reports [Sci Rep] 2018 May 17; Vol. 8 (1), pp. 7772. Date of Electronic Publication: 2018 May 17.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Endangered Species*, Amphibians/*microbiology , Chytridiomycota/*isolation & purification, Animals ; Information Dissemination ; Larva/microbiology ; Software
مستخلص: Parasitic chytrid fungi have emerged as a significant threat to amphibian species worldwide, necessitating the development of techniques to isolate these pathogens into culture for research purposes. However, early methods of isolating chytrids from their hosts relied on killing amphibians. We modified a pre-existing protocol for isolating chytrids from infected animals to use toe clips and biopsies from toe webbing rather than euthanizing hosts, and distributed the protocol to researchers as part of the BiodivERsA project RACE; here called the RML protocol. In tandem, we developed a lethal procedure for isolating chytrids from tadpole mouthparts. Reviewing a database of use a decade after their inception, we find that these methods have been applied across 5 continents, 23 countries and in 62 amphibian species. Isolation of chytrids by the non-lethal RML protocol occured in 18% of attempts with 207 fungal isolates and three species of chytrid being recovered. Isolation of chytrids from tadpoles occured in 43% of attempts with 334 fungal isolates of one species (Batrachochytrium dendrobatidis) being recovered. Together, these methods have resulted in a significant reduction and refinement of our use of threatened amphibian species and have improved our ability to work with this group of emerging pathogens.
References: Dis Aquat Organ. 2003 Aug 15;56(1):59-64. (PMID: 14524502)
ILAR J. 2007;48(3):255-9. (PMID: 17592187)
PLoS One. 2018 Jan 10;13(1):e0190955. (PMID: 29320551)
PLoS One. 2011;6(8):e23179. (PMID: 21887238)
Adv Genet. 2017;100:73-140. (PMID: 29153405)
Dis Aquat Organ. 2005 Dec 30;68(1):89-90. (PMID: 16465838)
F1000Res. 2014 Aug 20;3:199. (PMID: 25485096)
Proc Natl Acad Sci U S A. 1998 Jul 21;95(15):9031-6. (PMID: 9671799)
Proc Natl Acad Sci U S A. 2013 Sep 17;110(38):15325-9. (PMID: 24003137)
Dis Aquat Organ. 2007 Sep 14;77(2):113-8. (PMID: 17972752)
PLoS One. 2009 Dec 22;4(12):e8408. (PMID: 20027316)
Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):6241-6. (PMID: 22454494)
PLoS Genet. 2013;9(8):e1003703. (PMID: 23966879)
Ecol Evol. 2015 Sep 02;5(18):4079-97. (PMID: 26445660)
J Am Assoc Lab Anim Sci. 2009 Sep;48(5):512-6. (PMID: 19807972)
Annu Rev Microbiol. 2009;63:291-310. (PMID: 19575560)
Mol Ecol. 2009 Feb;18(3):415-29. (PMID: 19161465)
Mol Ecol. 2016 Jul;25(13):2978-96. (PMID: 26939017)
Science. 2018 May 11;360(6389):621-627. (PMID: 29748278)
Mol Ecol. 2012 Jul;21(13):3110-20. (PMID: 22332717)
Mol Ecol. 2013 Aug;22(16):4196-4209. (PMID: 23802586)
Dis Aquat Organ. 2004 Aug 9;60(2):141-8. (PMID: 15460858)
Science. 2006 Jul 7;313(5783):48. (PMID: 16825553)
PLoS Pathog. 2009 May;5(5):e1000458. (PMID: 19478871)
Nature. 2012 Apr 11;484(7393):186-94. (PMID: 22498624)
PLoS One. 2009 Sep 16;4(9):e6968. (PMID: 19756138)
Curr Biol. 2008 Sep 23;18(18):R853-4. (PMID: 18812076)
Science. 2004 Dec 3;306(5702):1783-6. (PMID: 15486254)
Sci Rep. 2015 Jan 27;5:8037. (PMID: 25623498)
Nature. 2005 Jan 20;433(7023):193. (PMID: 15662387)
Nature. 2004 Sep 23;431(7007):403. (PMID: 15385991)
Microb Biotechnol. 2017 Mar;10 (2):381-394. (PMID: 27995742)
Mol Ecol. 2012 Nov;21(21):5162-77. (PMID: 22857789)
Sci Rep. 2017 Mar 13;7:44443. (PMID: 28287614)
Proc Natl Acad Sci U S A. 2011 Nov 15;108(46):18732-6. (PMID: 22065772)
Mycologia. 2004 Jan-Feb;96(1):9-15. (PMID: 21148822)
Philos Trans R Soc Lond B Biol Sci. 2016 Dec 5;371(1709):. (PMID: 28080996)
Curr Biol. 2014 Jan 20;24(2):176-80. (PMID: 24374305)
PLoS One. 2012;7(11):e49924. (PMID: 23185485)
Dis Aquat Organ. 2008 Jul 7;80(2):85-94. (PMID: 18717061)
J Clin Microbiol. 2013 Dec;51(12):4173-7. (PMID: 24108616)
Nat Commun. 2017 Mar 21;8:14742. (PMID: 28322291)
معلومات مُعتمدة: MR/R015600/1 United Kingdom MRC_ Medical Research Council
تواريخ الأحداث: Date Created: 20180519 Date Completed: 20191220 Latest Revision: 20210109
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC5958081
DOI: 10.1038/s41598-018-24472-2
PMID: 29773857
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-018-24472-2