دورية أكاديمية
Pregnancy and the apoptotic pathway in experimental melanoma.
| العنوان: | Pregnancy and the apoptotic pathway in experimental melanoma. |
|---|---|
| المؤلفون: | Facina AS; Departments of Dermatology., Facina G; Gynecology, Molecular Gynecology Laboratory., Guerreiro da Silva IDC; Gynecology, Molecular Gynecology Laboratory., Corrêa SAA; Graduate Program in Public Health, Facvest University Center (UNIFACVEST), Lages, Santa Catarina, Brazil., Alexandre SM; Obstetrics., Logullo ÂF; Anatomic Pathology, Federal University of São Paulo (UNIFESP), São Paulo., Hosomi JK; Obstetrics., Nakamura MU; Obstetrics. |
| المصدر: | Melanoma research [Melanoma Res] 2018 Aug; Vol. 28 (4), pp. 286-294. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Lippincott Williams & Wilkins Country of Publication: England NLM ID: 9109623 Publication Model: Print Cited Medium: Internet ISSN: 1473-5636 (Electronic) Linking ISSN: 09608931 NLM ISO Abbreviation: Melanoma Res Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: London : Lippincott Williams & Wilkins Original Publication: Oxford, UK : Rapid Communications of Oxford, 1991- |
| مواضيع طبية MeSH: | Gene Expression/*genetics , Melanoma, Experimental/*genetics, Animals ; Apoptosis ; Cell Culture Techniques ; Female ; Humans ; Melanoma, Experimental/pathology ; Mice ; Pregnancy |
| مستخلص: | Pregnancy-associated melanoma is defined as melanoma diagnosed during pregnancy or within 1 year of delivery. The association of pregnancy with melanoma is well known, but its underlying molecular mechanisms of association are poorly understood. The aim was to assess the expression of apoptosis-related genes in melanoma tumors during pregnancy in an attempt to elucidate the molecular mechanisms underlying apoptosis-driven activation of melanoma cells in this period. Mice were allocated across two experimental groups (nonpregnant and pregnant) and implanted with the melanoma cell line BF16-F10. Tumor tissue was collected for RNA extraction and purification, and gene expression was quantified using the mouse apoptosis RT2ProfilerTM PCR array. Different intracellular apoptotic pathways were activated (positively or negatively) by pregnancy in tumor cells: intrinsic (21.5%), extrinsic (32%), caspase (14%), apoptosis (21.5%), and caspase-activated DNase (11%). The proportion of upregulated genes for each of these pathways was 100, 30, 50, 17, and 0%, respectively. MetaCore software was then used to analyze gene ontology processes and pathways by building networks. Among the gene ontology processes, the majority of differentiated genes were related to the apoptotic process. The main pathway activated by pregnancy was the intrinsic one (genes Api-5, Bcl2-L1, Birc-2, Birc-3, Bok, and Trp53bp2). Pregnancy activates the intrinsic apoptosis pathway to stimulate caspases 7 and 9, but the final balance is inhibition of apoptosis mechanisms. In mice, pregnancy cannot promote or worsen melanoma. |
| تواريخ الأحداث: | Date Created: 20180522 Date Completed: 20190201 Latest Revision: 20190201 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1097/CMR.0000000000000452 |
| PMID: | 29781870 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder كن أول من يترك تعليقا!