دورية أكاديمية
Protocols for Studies on Genetically Engineered Mouse Models in Prostate Cancer.
| العنوان: | Protocols for Studies on Genetically Engineered Mouse Models in Prostate Cancer. |
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| المؤلفون: | Armstrong CWD; Centre for Cancer Research and Cell Biology (CCRCB), Queen's University Belfast, Belfast, UK., Lyubomska O; Centre for Cancer Research and Cell Biology (CCRCB), Queen's University Belfast, Belfast, UK., LaBonte MJ; Centre for Cancer Research and Cell Biology (CCRCB), Queen's University Belfast, Belfast, UK., Waugh DJJ; Centre for Cancer Research and Cell Biology (CCRCB), Queen's University Belfast, Belfast, UK. d.waugh@qub.ac.uk. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1786, pp. 195-206. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Disease Models, Animal* , Mice, Transgenic*, Prostatic Neoplasms/*genetics , Prostatic Neoplasms/*pathology, Animals ; Breeding ; Cell Line, Tumor ; Gene Knockout Techniques ; Gene Targeting ; Genotype ; Humans ; Male ; Mice ; Recombination, Genetic ; Transplantation, Isogeneic |
| مستخلص: | Cancer studies have entered an era that is heavily focused on the contribution of the tumor microenvironment. For this reason, in vivo experimentation in an immunodeficient model system is no longer fit for purpose. As a consequence, numerous genetically engineered mouse models (GEMMs) which self-develop tumors have been developed to allow experiments to be performed in a fully immunocompetent setting. One of the most commonly used technologies is Cre-loxP recombination due to its unique ability to control target gene expression in a specified tissue type. However, the major limitation of these models remains the inability to generate sufficient numbers of age-matched mice for a synchronized experimental start date. For this reason, the derivation of cell lines from genetically modified murine prostate tissue is desirable and allows for the generation of syngeneic models via subcutaneous or orthotopic injection. |
| فهرسة مساهمة: | Keywords: Cell line derivation; Cre-loxP recombination; GEMMs; Genotyping; Mouse prostate dissection; Prostate cancer; Syngeneic |
| تواريخ الأحداث: | Date Created: 20180523 Date Completed: 20190212 Latest Revision: 20190215 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1007/978-1-4939-7845-8_11 |
| PMID: | 29786794 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-6029 |
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| DOI: | 10.1007/978-1-4939-7845-8_11 |