دورية أكاديمية
أعرض في EDS

A lectin of a non-invasive apple snail as an egg defense against predation alters the rat gut morphophysiology.

التفاصيل البيبلوغرافية
العنوان: A lectin of a non-invasive apple snail as an egg defense against predation alters the rat gut morphophysiology.
المؤلفون: Ituarte S; Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), Universidad Nacional de La Plata (UNLP)-CONICET, La Plata, Argentina., Brola TR; Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), Universidad Nacional de La Plata (UNLP)-CONICET, La Plata, Argentina., Fernández PE; Instituto de Patología B. Epstein, Cátedra de Patología General Veterinaria, Facultad Ciencias Veterinarias, UNLP, La Plata, Argentina., Mu H; Department of Biology, Hong Kong Baptist University, Hong Kong, China., Qiu JW; Department of Biology, Hong Kong Baptist University, Hong Kong, China., Heras H; Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), Universidad Nacional de La Plata (UNLP)-CONICET, La Plata, Argentina.; Cátedra de Química Biológica, Facultad de Ciencias Naturales y Museo, UNLP, La Plata, Argentina., Dreon MS; Instituto de Investigaciones Bioquímicas de La Plata (INIBIOLP), Universidad Nacional de La Plata (UNLP)-CONICET, La Plata, Argentina.; Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, UNLP, La Plata, Argentina.
المصدر: PloS one [PLoS One] 2018 Jun 01; Vol. 13 (6), pp. e0198361. Date of Electronic Publication: 2018 Jun 01 (Print Publication: 2018).
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Public Library of Science Country of Publication: United States NLM ID: 101285081 Publication Model: eCollection Cited Medium: Internet ISSN: 1932-6203 (Electronic) Linking ISSN: 19326203 NLM ISO Abbreviation: PLoS One Subsets: MEDLINE
أسماء مطبوعة: Original Publication: San Francisco, CA : Public Library of Science
مواضيع طبية MeSH: Eggs* , Gastrointestinal Tract*/anatomy & histology , Gastrointestinal Tract*/drug effects , Gastrointestinal Tract*/physiology , Predatory Behavior*/drug effects , Rats*/anatomy & histology , Rats*/physiology, Egg Proteins/*physiology , Lectins/*physiology , Snails/*chemistry, Agglutination ; Animals ; Caco-2 Cells ; Cells, Cultured ; Humans ; Intestines/anatomy & histology ; Intestines/drug effects ; Intestines/physiology ; Lectins/pharmacology ; Male ; Rats, Wistar
مستخلص: The eggs of the freshwater Pomacea apple snails develop above the water level, exposed to varied physical and biological stressors. Their high hatching success seems to be linked to their proteins or perivitellins, which surround the developing embryo providing nutrients, sunscreens and varied defenses. The defensive mechanism has been unveiled in P. canaliculata and P. maculata eggs, where their major perivitellins are pigmented, non-digestible and provide a warning coloration while another perivitellin acts as a toxin. In P. scalaris, a species sympatric to the former, the defense strategy seems different, since no toxin was found and the major perivitellin, PsSC, while also colored and non-digestible, is a carbohydrate-binding protein. In this study we examine the structure and function of PsSC by sequencing its subunits, characterizing its carbohydrate binding profile and evaluating its effect on gut cells. Whereas cDNA sequencing and database search showed no lectin domain, glycan array carbohydrate binding profile revealed a strong specificity for glycosphingolipids and ABO group antigens. Moreover, PsSC agglutinated bacteria in a dose-dependent manner. Inspired on the defensive properties of seed lectins we evaluated the effects of PsSC on intestinal cells both in vitro (Caco-2 and IEC-6 cells) and in the gastrointestinal tract of rats. PsSC binds to Caco-2 cell membranes without reducing its viability, while a PsSC-containing diet temporarily induces large epithelium alterations and an increased absorptive surface. Based on these results, we propose that PsSC is involved in embryo defenses by altering the gut morphophysiology of potential predators, a convergent role to plant defensive lectins.
Competing Interests: The authors have declared that no competing interests exist.
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المشرفين على المادة: 0 (Egg Proteins)
0 (Lectins)
تواريخ الأحداث: Date Created: 20180602 Date Completed: 20190111 Latest Revision: 20240326
رمز التحديث: 20240326
مُعرف محوري في PubMed: PMC5983499
DOI: 10.1371/journal.pone.0198361
PMID: 29856808
قاعدة البيانات: MEDLINE
الوصف
تدمد:1932-6203
DOI:10.1371/journal.pone.0198361