دورية أكاديمية
أعرض في EDS

The effects of local administration of mesenchymal stem cells on rat corneal allograft rejection.

التفاصيل البيبلوغرافية
العنوان: The effects of local administration of mesenchymal stem cells on rat corneal allograft rejection.
المؤلفون: Jia Z; Tianjin Medical University Eye Hospital, Tianjin Medical University Eye Institute & Tianjin Medical University School of Optometry and Ophthalmology, No. 251, Fukang R., Nankai Dist, Tianjin, China., Li F; Tianjin Medical University Eye Hospital, Tianjin Medical University Eye Institute & Tianjin Medical University School of Optometry and Ophthalmology, No. 251, Fukang R., Nankai Dist, Tianjin, China., Zeng X; Tianjin Medical University Eye Hospital, Tianjin Medical University Eye Institute & Tianjin Medical University School of Optometry and Ophthalmology, No. 251, Fukang R., Nankai Dist, Tianjin, China., Lv Y; Tianjin Medical University Eye Hospital, Tianjin Medical University Eye Institute & Tianjin Medical University School of Optometry and Ophthalmology, No. 251, Fukang R., Nankai Dist, Tianjin, China., Zhao S; Tianjin Medical University Eye Hospital, Tianjin Medical University Eye Institute & Tianjin Medical University School of Optometry and Ophthalmology, No. 251, Fukang R., Nankai Dist, Tianjin, China. doctorzsz@yeah.net.
المصدر: BMC ophthalmology [BMC Ophthalmol] 2018 Jun 08; Vol. 18 (1), pp. 139. Date of Electronic Publication: 2018 Jun 08.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 100967802 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2415 (Electronic) Linking ISSN: 14712415 NLM ISO Abbreviation: BMC Ophthalmol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : BioMed Central, [2001-
مواضيع طبية MeSH: Graft Survival*, Corneal Transplantation/*adverse effects , Graft Rejection/*prevention & control , Mesenchymal Stem Cell Transplantation/*methods , Mesenchymal Stem Cells/*cytology, Allografts ; Animals ; Disease Models, Animal ; Female ; Graft Rejection/diagnosis ; Microscopy, Acoustic ; Rats ; Rats, Wistar
مستخلص: Background: Mesenchymal stem cells (MSCs) have been reported to promote long-term cellular and organ transplant acceptance due to their immunotherapeutic characteristics. Previous work from our lab using a rat allograft model has shown that systemic infusion of MSCs inhibited corneal allograft rejection and prolonged graft survival. Here, we further investigated the effects of local MSCs administration in the same animal model.
Methods: Donor-derived MSCs were isolated and cultured while corneal grafts obtained from Wistar rats were transplanted into Lewis rat hosts. Hosts were then randomly separated into four groups and treated with previously cultured MSCs at different times and doses. Graft survival was clinically assessed using slit-lamp biomicroscopy and the median survival time (MST) was calculated. Grafts were examined histologically using hematoxylin-eosin (H-E) staining and immunohistochemically using antibodies against CD4. A comprehensive graft analysis of IL-2, IL-4, IL-10, and IFN-γ expression was also conducted using both real-time polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA).
Results: Postoperative MSCs injection prolonged graft survival time when compared with controls (MST 9.8 ± 1.2 days). Injection twice of MSCs (MST 12.6 ± 1.4 days) was more effective than a single injection (MST 10.8 ± 1.3 days). MSCs-treated groups also showed suppression of inflammatory cell as well as CD4 + T cell infiltration in the allograft region. IL-4 and IL-10 levels were significantly increased in grafts obtained from postoperative twice MSCs-treated rats when compared with controls. There were no significant differences in IL-2 or IFN-γ expression across groups.
Conclusions: Subconjunctival injection of MSCs in rats was effective in prolonging corneal allograft survival. This effect was mediated by inhibition of inflammatory and immune responses, indicating an anti-inflammatory shift in the balance of T helper (Th)1 to T helper(Th) 2.
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معلومات مُعتمدة: 16JQNJC12500 Tianjin Municipal Science and Technology Commission Youth Project
فهرسة مساهمة: Keywords: Cell-based immunomodulatory therapy; Corneal allograft rejection; Local administration; Mesenchymal stem cells
تواريخ الأحداث: Date Created: 20180610 Date Completed: 20180620 Latest Revision: 20181202
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC5994063
DOI: 10.1186/s12886-018-0802-6
PMID: 29884142
قاعدة البيانات: MEDLINE
الوصف
تدمد:1471-2415
DOI:10.1186/s12886-018-0802-6