دورية أكاديمية
Utilization of Ultrasound Guided Tissue-directed Cellular Implantation for the Establishment of Biologically Relevant Metastatic Tumor Xenografts.
| العنوان: | Utilization of Ultrasound Guided Tissue-directed Cellular Implantation for the Establishment of Biologically Relevant Metastatic Tumor Xenografts. |
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| المؤلفون: | Thomas TT; Departments of Surgery, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School., Chukkapalli S; Departments of Surgery, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School., Van Noord RA; Departments of Surgery, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School., Krook M; Departments of Pathology, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School., Hoenerhoff MJ; Unit for Laboratory Animal Medicine, The University of Michigan Medical School., Dillman JR; Departments of Radiology, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School., Lawlor ER; Departments of Pathology, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School; Departments of Pediatrics, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School., Opipari VP; Departments of Pediatrics, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School., Newman EA; Departments of Surgery, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School; eanewman@med.umich.edu. |
| المصدر: | Journal of visualized experiments : JoVE [J Vis Exp] 2018 May 25 (135). Date of Electronic Publication: 2018 May 25. |
| نوع المنشور: | Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Video-Audio Media |
| اللغة: | English |
| بيانات الدورية: | Publisher: MYJoVE Corporation Country of Publication: United States NLM ID: 101313252 Publication Model: Electronic Cited Medium: Internet ISSN: 1940-087X (Electronic) Linking ISSN: 1940087X NLM ISO Abbreviation: J Vis Exp Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: [Boston, Mass. : MYJoVE Corporation, 2006]- |
| مواضيع طبية MeSH: | Transplantation, Heterologous/*methods , Tumor Microenvironment/*genetics , Ultrasonography/*methods, Animals ; Cell Line, Tumor ; Disease Models, Animal ; Humans ; Mice ; Neoplasm Metastasis |
| مستخلص: | Preclinical testing of anticancer therapies relies on relevant xenograft models that mimic the innate tendencies of cancer. Advantages of standard subcutaneous flank models include procedural ease and the ability to monitor tumor progression and response without invasive imaging. Such models are often inconsistent in translational clinical trials and have limited biologically relevant characteristics with low proclivity to produce metastasis, as there is a lack of a native microenvironment. In comparison, orthotopic xenograft models at native tumor sites have been shown to mimic the tumor microenvironment and replicate important disease characteristics such as distant metastatic spread. These models often require tedious surgical procedures with prolonged anesthetic time and recovery periods. To address this, cancer researchers have recently utilized ultrasound-guided injection techniques to establish cancer xenograft models for preclinical experiments, which allows for rapid and reliable establishment of tissue-directed murine models. Ultrasound visualization also provides a noninvasive method for longitudinal assessment of tumor engraftment and growth. Here, we describe the method for ultrasound-guided injection of cancer cells, utilizing the adrenal gland for NB and renal sub capsule for ES. This minimally invasive approach overcomes tedious open surgery implantation of cancer cells in tissue-specific locations for growth and metastasis, and abates morbid recovery periods. We describe the utilization of both established cell lines and patient derived cell lines for orthotopic injection. Pre-made commercial kits are available for tumor dissociation and luciferase tagging of cells. Injection of cell suspension using image-guidance provides a minimally invasive and reproducible platform for the creation of preclinical models. This method is utilized to create reliable preclinical models for other cancers such as bladder, liver and pancreas exemplifying its untapped potential for numerous cancer models. |
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| معلومات مُعتمدة: | K26 OD016502 United States OD NIH HHS; P30 CA046592 United States CA NCI NIH HHS; T32 CA009676 United States CA NCI NIH HHS |
| تواريخ الأحداث: | Date Created: 20180612 Date Completed: 20180806 Latest Revision: 20190610 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC6101425 |
| DOI: | 10.3791/57558 |
| PMID: | 29889191 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1940-087X |
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| DOI: | 10.3791/57558 |