دورية أكاديمية

Cytoprotective role of vitamin E in porcine adipose-tissue-derived mesenchymal stem cells against hydrogen-peroxide-induced oxidative stress.

التفاصيل البيبلوغرافية
العنوان: Cytoprotective role of vitamin E in porcine adipose-tissue-derived mesenchymal stem cells against hydrogen-peroxide-induced oxidative stress.
المؤلفون: Bhatti FUR; Department of Orthopaedic Surgery and Biomedical Engineering, University of Tennessee Health Science Center-Campbell Clinic, Memphis, TN, USA.; Memphis VA Medical Center, Memphis, TN, USA., Kim SJ; Kongju National University, Gongju, South Korea., Yi AK; Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, Memphis, TN, USA., Hasty KA; Department of Orthopaedic Surgery and Biomedical Engineering, University of Tennessee Health Science Center-Campbell Clinic, Memphis, TN, USA. khasty@uthsc.edu.; Memphis VA Medical Center, Memphis, TN, USA. khasty@uthsc.edu., Cho H; Department of Orthopaedic Surgery and Biomedical Engineering, University of Tennessee Health Science Center-Campbell Clinic, Memphis, TN, USA. hcho4@uthsc.edu.; Memphis VA Medical Center, Memphis, TN, USA. hcho4@uthsc.edu.
المصدر: Cell and tissue research [Cell Tissue Res] 2018 Oct; Vol. 374 (1), pp. 111-120. Date of Electronic Publication: 2018 Jun 27.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer-Verlag Country of Publication: Germany NLM ID: 0417625 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1432-0878 (Electronic) Linking ISSN: 0302766X NLM ISO Abbreviation: Cell Tissue Res Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Berlin, New York, Springer-Verlag.
مواضيع طبية MeSH: Adipose Tissue/*metabolism , Hydrogen Peroxide/*adverse effects , Mesenchymal Stem Cells/*metabolism , Oxidative Stress/*drug effects , Vitamin E/*therapeutic use, Animals ; Disease Models, Animal ; Swine ; Vitamin E/pharmacology
مستخلص: Survival of mesenchymal stem cells (MSCs) against oxidative stress and inflammation is vital for effective stem cell therapy. The reactive oxygen species (ROS) result in apoptosis and release of inflammatory mediators. Adipose-derived stem cells (ASCs) have shown promise for stem cell therapy owing to their anti-inflammatory and anti-oxidant activity. Previously, we showed the benefits of vitamin E against hydrogen peroxide (H 2 O 2 )-induced oxidative stress in rat bone marrow-derived MSCs. In this study, we aim to evaluate the effect of vitamin E treatment on porcine adipose-derived mesenchymal stem cells (pASCs) against H 2 O 2 -induced oxidative stress. The oxidative stress was induced by treating pASCs with 500 μM H 2 O 2 with or without vitamin E. Viability of pASCs is enhanced after vitamin E treatment. In addition, reduced cellular toxicity, total NO level, PGE 2 production and caspase-3 activity were observed after vitamin E treatment. Gene expression analysis of vitamin E-treated pASCs showed down-regulated expression for the genes associated with oxidative stress and apoptosis, viz., NOS2, Casp3, p53, BAX, MDM2, NFκB, HIF1α and VEGF-A genes. On the other hand, expression of anti-apoptotic and survival genes was up-regulated, viz., BCL2, BCL2L1 and MCL1. Furthermore, phosphorylation of Akt was attenuated following vitamin E treatment. The findings of this study may help in developing effective stem cell therapy for the diseases characterized by the oxidative stress and inflammation.
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معلومات مُعتمدة: IRG-5942 Arthritis Foundation; AR064723 NIH/NIAMS; R01 AR064723 United States AR NIAMS NIH HHS; I01 BX002392 United States BX BLRD VA; MERIT (1I01BX004151) United States VA VA; I01 BX004151 United States BX BLRD VA; Resaerch Grant Oxnard Foundation
فهرسة مساهمة: Keywords: Alpha-Tocopherol; Hydrogen peroxide; Mesenchymal stem cells; Oxidative stress; Porcine
المشرفين على المادة: 1406-18-4 (Vitamin E)
BBX060AN9V (Hydrogen Peroxide)
تواريخ الأحداث: Date Created: 20180629 Date Completed: 20181218 Latest Revision: 20210823
رمز التحديث: 20221213
مُعرف محوري في PubMed: PMC7036264
DOI: 10.1007/s00441-018-2857-3
PMID: 29951700
قاعدة البيانات: MEDLINE
الوصف
تدمد:1432-0878
DOI:10.1007/s00441-018-2857-3