دورية أكاديمية
[Lipoilcarnosine: synthesis, study of physico-chemical and antioxidant properties, biological activity].
| العنوان: | [Lipoilcarnosine: synthesis, study of physico-chemical and antioxidant properties, biological activity]. |
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| عنوان ترانسليتريتد: | Lipoilkarnozin: sintez, izuchenie fiziko-khimicheskikh i antioksidantnykh svoĭstv, biologicheskaia aktivnost'. |
| المؤلفون: | Stvolinsky SL; Research Center of Neurology, Moscow, Russia., Antonova NA; Moscow Technological University, Moscow, Russia., Kulikova OI; Research Center of Neurology, Moscow, Russia; Peoples' Friendship University of Russia, Moscow, Russia., Lopachev AV; Research Center of Neurology, Moscow, Russia., Abaimov DA; Research Center of Neurology, Moscow, Russia., Al-Baidani I; Moscow Technological University, Moscow, Russia., Lopacheva OM; Research Center of Neurology, Moscow, Russia; Lomonosov Moscow State University, International Biotechnological Center, Moscow, Russia., Fedorova TN; Research Center of Neurology, Moscow, Russia., Kaplun AP; Moscow Technological University, Moscow, Russia., Sorokoumova GM; Moscow Technological University, Moscow, Russia. |
| المصدر: | Biomeditsinskaia khimiia [Biomed Khim] 2018 Jun; Vol. 64 (3), pp. 268-275. |
| نوع المنشور: | Journal Article |
| اللغة: | Russian |
| بيانات الدورية: | Publisher: Rossiĭskai︠a︡ akademii︠a︡ medit︠s︡inskikh nauk Country of Publication: Russia (Federation) NLM ID: 101196966 Publication Model: Print Cited Medium: Print ISSN: 2310-6972 (Print) Linking ISSN: 23106905 NLM ISO Abbreviation: Biomed Khim Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Moskva : Rossiĭskai︠a︡ akademii︠a︡ medit︠s︡inskikh nauk, 2003- |
| مواضيع طبية MeSH: | Antioxidants*/chemical synthesis , Antioxidants*/chemistry , Antioxidants*/pharmacology , Carnosine*/analogs & derivatives , Carnosine*/chemical synthesis , Carnosine*/chemistry , Carnosine*/pharmacology, MPTP Poisoning/*drug therapy, Cell Line, Tumor ; Cell Survival/drug effects ; Humans ; MPTP Poisoning/metabolism ; MPTP Poisoning/pathology |
| مستخلص: | Synthesis of lipoilcarnosine (LipC) - a conjugated molecule based on two natural antioxidants, carnosine and a-lipoic acid, is described. Its physico-chemical, antioxidant properties and biological activity are characterized. According to reversed-phase HPLC with a UV detector, purity of the final product was 89.3%. The individuality of the obtained sodium salt of LipC was confirmed by tandem HPLC-mass spectrometry. High resistance of LipC to hydrolysis with serum carnosinase was demonstrated. The antioxidant activity of LipC measured by reaction with the formation of thiobarbituric acid reacting substances and kinetic parameters of iron-induced chemiluminescence was higher than that of carnosine and lipoic acid. LipC did not affect viability of SH-SY5Y human neuroblastoma culture cells, differentiated towards the dopaminergic type, at concentrations not exceeding 5 mM. At the concentration range of 0.1-0.25 mM LipC protected neuronal cells against 1-methyl-4-phenylpyridinium (MPP + )-induced toxicity. |
| فهرسة مساهمة: | Keywords: a-lipoic acid; antioxidant; carnosinase; carnosine; lipoilcarnosine; parkinsonism Local Abstract: [Publisher, Russian] Opisano poluchenie lipoilkarnozina (LipK) – kon"iugirovannoĭ molekuly na osnove dvukh prirodnykh antioksidantov karnozina i a-lipoevoĭ kisloty i okharakterizovany ego fiziko-khimicheskie, antioksidantnye svoĭstva i biologicheskaia aktivnost'. Po dannym obrashchenno-fazovoĭ VÉZhKh s ispol'zovaniem UF detektora chistota poluchennogo konechnogo produkta sostavila 89,3%. Metodom tandemnoĭ VÉZhKh-mass-spektrometrii byla podtverzhdena individual'nost' poluchennoĭ natrievoĭ soli LipK. LipK proiavlial vysokuiu ustoĭchivost' k gidrolizu syvorotochnoĭ karnozinazoĭ i kharakterizovalsia bol'sheĭ, chem u karnozina i lipoevoĭ kisloty antioksidantnoĭ aktivnost'iu po reaktsii s obrazovaniem produktov, reagiruiushchikh s tiobarbiturovoĭ kislotoĭ, i po kineticheskim parametram zhelezo-indutsirovannoĭ khemiliuminestsentsii. LipK v kontsentratsiiakh, ne prevyshaiushchikh 5 mM, ne vliial na zhiznesposobnost' intaktnykh kletok kul'tury neĭroblastomy cheloveka SH-SY5Y, differentsirovannoĭ po dofaminergicheskomu tipu, a v diapazone kontsentratsiĭ 0,1-0,25 mM obespechival tsitoprotektornoe deĭstvie v usloviiakh modelirovaniia na kul'ture kletok 1-metil-4-fenilpiridiniĭ (MFP + )-indutsirovannoĭ toksichnosti. |
| المشرفين على المادة: | 0 (Antioxidants) 8HO6PVN24W (Carnosine) |
| تواريخ الأحداث: | Date Created: 20180703 Date Completed: 20181016 Latest Revision: 20181023 |
| رمز التحديث: | 20240628 |
| DOI: | 10.18097/PBMC20186403268 |
| PMID: | 29964264 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 2310-6972 |
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| DOI: | 10.18097/PBMC20186403268 |