دورية أكاديمية
Identification of key determinants in Porphyromonas gingivalis host-cell invasion assays.
| العنوان: | Identification of key determinants in Porphyromonas gingivalis host-cell invasion assays. |
|---|---|
| المؤلفون: | Al-Taweel FBH; Department of Periodontics, College of Dentistry, University of Baghdad, Baghdad, Iraq., Al-Magsoosi MJN; Department of Oral Diagnosis, College of Dentistry, University of Basra, Basra, Iraq., Douglas CWI; Academic Unit of Oral & Maxillofacial Medicine, Surgery & Pathology, School of Clinical Dentistry, University of Sheffield, Sheffield, UK., Whawell SA; Academic Unit of Oral & Maxillofacial Medicine, Surgery & Pathology, School of Clinical Dentistry, University of Sheffield, Sheffield, UK. |
| المصدر: | European journal of oral sciences [Eur J Oral Sci] 2018 Oct; Vol. 126 (5), pp. 367-372. Date of Electronic Publication: 2018 Aug 02. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Blackwell Country of Publication: England NLM ID: 9504563 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1600-0722 (Electronic) Linking ISSN: 09098836 NLM ISO Abbreviation: Eur J Oral Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Chichester : Wiley-Blackwell Original Publication: Copenhagen : Munksgaard, c1995- |
| مواضيع طبية MeSH: | Host-Pathogen Interactions/*physiology , Porphyromonas gingivalis/*growth & development , Porphyromonas gingivalis/*pathogenicity, Bacterial Adhesion/drug effects ; Carcinoma, Squamous Cell ; Cell Line, Tumor ; Cells, Cultured ; DNA, Bacterial ; Fibronectins/pharmacology ; Humans ; Periodontal Diseases/microbiology ; Stem Cells |
| مستخلص: | The periodontal pathogen Porphyromonas gingivalis can invade host cells, a virulence trait which may contribute to the persistence of infection at subgingival sites. Whilst the antibiotic protection assay has been commonly employed to investigate and quantify P. gingivalis invasion, data obtained have varied widely and a thorough investigation of the factors influencing this is lacking. We investigated the role of a number of bacterial and host-cell factors and report that the growth phase of P. gingivalis, source (laboratory strain vs. clinical strain), host-cell identity (cell line vs. primary), host-cell lysis method, and host-cell passage number had no significant effect on bacterial invasion. However, incubation time, host-cell seeding density, method of quantification (viable count vs. DNA), and whether host cells were plated or in suspension, were shown to influence invasion. Also, cells isolated by rapid adhesion to fibronectin exhibited higher levels of P. gingivalis invasion, possibly as a result of increased levels of active α5β1 integrin. Interestingly, this may represent a population of cells with stem cell-like properties. This study provides important new information by identifying the most important factors that influence P. gingivalis invasion assays and may help to explain variations in the levels previously reported. (© 2018 Eur J Oral Sci.) |
| فهرسة مساهمة: | Keywords: integrins; keratinocytes; periodontal disease; stem cells; virulence |
| المشرفين على المادة: | 0 (DNA, Bacterial) 0 (Fibronectins) |
| تواريخ الأحداث: | Date Created: 20180803 Date Completed: 20190624 Latest Revision: 20190624 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1111/eos.12557 |
| PMID: | 30070725 |
| قاعدة البيانات: | MEDLINE |
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