دورية أكاديمية
أعرض في EDS

LPS-induces IL-6 and IL-8 gene expression in bovine endometrial cells "through DNA methylation".

التفاصيل البيبلوغرافية
العنوان: LPS-induces IL-6 and IL-8 gene expression in bovine endometrial cells "through DNA methylation".
المؤلفون: Wang J; College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, PR China., Yan X; College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, PR China., Nesengani LT; College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, PR China., Ding H; College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, PR China., Yang L; College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, PR China., Lu W; College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, PR China. Electronic address: wenfa2004@163.com.
المصدر: Gene [Gene] 2018 Nov 30; Vol. 677, pp. 266-272. Date of Electronic Publication: 2018 Aug 01.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier/North-Holland Country of Publication: Netherlands NLM ID: 7706761 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0038 (Electronic) Linking ISSN: 03781119 NLM ISO Abbreviation: Gene Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Amsterdam, Elsevier/North-Holland, 1976-
مواضيع طبية MeSH: DNA Methylation/*drug effects , DNA Methylation/*genetics , Interleukin-6/*genetics , Interleukin-8/*genetics , Lipopolysaccharides/*pharmacology, Animals ; Azacitidine/pharmacology ; Cattle ; Cells, Cultured ; DNA (Cytosine-5-)-Methyltransferase 1/genetics ; DNA (Cytosine-5-)-Methyltransferases/genetics ; DNA Modification Methylases/genetics ; Gene Expression/drug effects ; Gene Expression/genetics ; Promoter Regions, Genetic/drug effects ; Promoter Regions, Genetic/genetics ; RNA, Messenger/genetics ; DNA Methyltransferase 3B
مستخلص: Uterine infection in dairy cows causes great economic loss. In bovine endometrial cells, lipopolysaccharide (LPS)-stimulated increase in interleukin 6 (IL-6) and interleukin 8 (IL-8) mRNA is crucial for the inflammatory response; however, the regulatory mechanisms remain unclear. Here, we investigated the role of DNA methylation in IL-6 and IL-8 mRNA expression following LPS-induction in bovine endometrial cells. IL-6 and IL-8 mRNA expression was evaluated under DNA methylation inhibition using 5-Aza-2'-deoxycytodine (5Aza) following LPS stimulation. Expression of DNA methyltransferases (DNMT1, DNMT3A, and DNMT3B), methyl CpG-binding protein 2 (MeCP2) and DNA methylation at IL-6 and IL-8 regions, were analyzed using quantitative real-time PCR (qRT-PCR) and bisulfite sequencing PCR (BSP) following 24 h of LPS treatment. Inhibition of DNA methylation significantly enhanced LPS-induced IL-6 and IL-8 mRNA expression. LPS increased IL-6 and IL-8 mRNA expression, and decreased methylation levels of specific CpG sites at the IL-6 promoter (at -366 and -660) and the IL-8 promoter (at -120 and -48) after 24 h. Furthermore, LPS treatment for 24 h significantly increased DNMT1, DNMT3A, DNMT3B, and MeCP2 mRNA expression. Our results indicate that treating bovine endometrial cells with LPS induces the expression of IL-6 and IL-8 mRNA regulated by IL-6 and IL-8 promoter methylation.
(Copyright © 2018 Elsevier B.V. All rights reserved.)
فهرسة مساهمة: Keywords: Bovine endometrial cells; IL-6; IL-8; LPS; Methylation
المشرفين على المادة: 0 (Interleukin-6)
0 (Interleukin-8)
0 (Lipopolysaccharides)
0 (RNA, Messenger)
EC 2.1.1.- (DNA Modification Methylases)
EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferase 1)
EC 2.1.1.37 (DNA (Cytosine-5-)-Methyltransferases)
M801H13NRU (Azacitidine)
تواريخ الأحداث: Date Created: 20180805 Date Completed: 20180928 Latest Revision: 20231213
رمز التحديث: 20240628
DOI: 10.1016/j.gene.2018.07.074
PMID: 30077008
قاعدة البيانات: MEDLINE
الوصف
تدمد:1879-0038
DOI:10.1016/j.gene.2018.07.074