دورية أكاديمية
Ten Minute Stain to Detect Proteins in Polyacrylamide Electrophoresis Gels with Direct Red 81 and Amido Black.
| العنوان: | Ten Minute Stain to Detect Proteins in Polyacrylamide Electrophoresis Gels with Direct Red 81 and Amido Black. |
|---|---|
| المؤلفون: | Goldring JPD; Biochemistry, University of KwaZulu-Natal, Scottsville, South Africa. Goldringd@ukzn.ac.za., Krause RGE; Biochemistry, University of KwaZulu-Natal, Scottsville, South Africa. |
| المصدر: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2018; Vol. 1853, pp. 159-163. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Humana Press Country of Publication: United States NLM ID: 9214969 Publication Model: Print Cited Medium: Internet ISSN: 1940-6029 (Electronic) Linking ISSN: 10643745 NLM ISO Abbreviation: Methods Mol Biol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Totowa, NJ : Humana Press Original Publication: Clifton, N.J. : Humana Press, |
| مواضيع طبية MeSH: | Amido Black* , Azo Compounds* , Electrophoresis, Polyacrylamide Gel* , Proteins*/analysis , Staining and Labeling*/methods, Rosaniline Dyes |
| مستخلص: | Proteins separated by SDS-polyacrylamide gel electrophoresis need to be stained with organic dyes to be visualized. Once stained the intensity of each stained protein band can be used to compare the differences in protein concentration and to measure the relative concentration of any protein band. The most popular standard protein staining is with Coomassie Blue R-250 which takes an hour to stain proteins to saturation and several hours to remove background staining. Direct Red 81 and Amido Black stain proteins within 2.5 min and staining is complete by 10 min. Here the rapid staining of proteins with Direct Red 81 and Amido Black in comparison to staining with Coomassie Blue R-250 is described. |
| فهرسة مساهمة: | Keywords: Amido black; Coomassie blue; Direct Red 81; Protein staining |
| المشرفين على المادة: | 0 (Azo Compounds) 0 (Proteins) 0 (Rosaniline Dyes) 6104-59-2 (Coomassie brilliant blue R) 7E49Q56PNB (Direct Red 81) SZT789770M (Amido Black) |
| تواريخ الأحداث: | Date Created: 20180812 Date Completed: 20190513 Latest Revision: 20190513 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1007/978-1-4939-8745-0_19 |
| PMID: | 30097941 |
| قاعدة البيانات: | MEDLINE |
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