دورية أكاديمية
Molecular and immunohistochemical analysis of the urethra of female rats after induced trauma and intravenous therapy with muscle derived stem cells.
| العنوان: | Molecular and immunohistochemical analysis of the urethra of female rats after induced trauma and intravenous therapy with muscle derived stem cells. |
|---|---|
| المؤلفون: | Bilhar APM; Department of Gynecology, Federal University of São Paulo, São Paulo, Brazil., Bortolini MAT; Department of Gynecology, Federal University of São Paulo, São Paulo, Brazil., Sé AB; Department of Gynecology, Federal University of São Paulo, São Paulo, Brazil., Feitosa SM; Department of Gynecology, Federal University of São Paulo, São Paulo, Brazil., Salerno GRF; Department of Gynecology, Federal University of São Paulo, São Paulo, Brazil., Zanoteli E; Department of Neurology, University of São Paulo, São Paulo, Brazil., Simões MJ; Department of Gynecology, Federal University of São Paulo, São Paulo, Brazil., Castro RA; Department of Gynecology, Federal University of São Paulo, São Paulo, Brazil. |
| المصدر: | Neurourology and urodynamics [Neurourol Urodyn] 2018 Sep; Vol. 37 (7), pp. 2151-2159. Date of Electronic Publication: 2018 Aug 28. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Alan R. Liss Country of Publication: United States NLM ID: 8303326 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1520-6777 (Electronic) Linking ISSN: 07332467 NLM ISO Abbreviation: Neurourol Urodyn Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: New York : Alan R. Liss, c1982- |
| مواضيع طبية MeSH: | Stem Cell Transplantation*, Muscle, Skeletal/*cytology , Urethra/*metabolism , Vagina/*injuries, Animals ; Collagen/metabolism ; Disease Models, Animal ; Extracellular Matrix/metabolism ; Female ; Muscle, Skeletal/metabolism ; Rats ; Rats, Wistar |
| مستخلص: | Aims: To identify the urethral migration of muscle derived stem cells (MDSCs) after intravenous (IV) injection in rats that underwent vaginal distension (VD) and to analyze the effects of MDSC in the urethra of rats after trauma in regards to: (1) mRNA expression of collagens, Vegf, Ngf, Ki67, Myh11, and Myh2; (2) expression of smooth and striated muscle proteins. Methods: MDSCs expressing green fluorescent protein (GFP) were injected into the tail vein of rats 3 days after VD. The location of GFP cells was verified at 2 h and at 7 days following IV injection. Urethras of three groups were analyzed: Control, Trauma 7D, and MDSC 7D. Real-time RT-qPCR and immunohistochemistry were performed. Results: MDSCs were identified only after 2 h of the procedure in the urethra. Myh11 gene was overexpressed in the Trauma group in relation to Control. Ki67 gene expression was increased in the MDSC group relative to Trauma and Control. Col1a1 and Col3a1 genes expression were increased in the MDSC group relative to Control. Ngf mRNA level was decreased in the MDSC group in relation to Trauma. Protein expression of Mhy11, Myh2, and Desmin were increased in the MDSC group in relation to Trauma and decreased in the Trauma in relation to Control. Conclusion: MDSCs migrated early to the traumatized urethra, but did not integrate into the tissue. MDSC alters the expression of genes related to cell proliferation, neural growth factor and extracellular matrix and the expression of smooth and striated muscle proteins in the traumatized rat urethra. (© 2018 Wiley Periodicals, Inc.) |
| فهرسة مساهمة: | Keywords: adult stem cell; regenerative medicine; urethra; urinary incontinence |
| المشرفين على المادة: | 9007-34-5 (Collagen) |
| تواريخ الأحداث: | Date Created: 20180829 Date Completed: 20191011 Latest Revision: 20191011 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1002/nau.23567 |
| PMID: | 30152522 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 1520-6777 |
|---|---|
| DOI: | 10.1002/nau.23567 |