دورية أكاديمية
Neuropeptides encoded by nlp-49 modulate locomotion, arousal and egg-laying behaviours in Caenorhabditis elegans via the receptor SEB-3.
| العنوان: | Neuropeptides encoded by nlp-49 modulate locomotion, arousal and egg-laying behaviours in Caenorhabditis elegans via the receptor SEB-3. |
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| المؤلفون: | Chew YL; Neurobiology Division, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK., Grundy LJ; Neurobiology Division, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK., Brown AEX; MRC London Institute of Medical Sciences, London W12 0NN, UK.; Institute of Clinical Sciences, Imperial College London, London W12 0NN, UK., Beets I; Cell Biology Division, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK isabel.beets@kuleuven.be.; Department of Biology, KU Leuven, 3000 Leuven, Belgium., Schafer WR; Neurobiology Division, MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, UK wschafer@mrc-lmb.cam.ac.uk. |
| المصدر: | Philosophical transactions of the Royal Society of London. Series B, Biological sciences [Philos Trans R Soc Lond B Biol Sci] 2018 Sep 10; Vol. 373 (1758). Date of Electronic Publication: 2018 Sep 10. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royal Society Country of Publication: England NLM ID: 7503623 Publication Model: Electronic Cited Medium: Internet ISSN: 1471-2970 (Electronic) Linking ISSN: 09628436 NLM ISO Abbreviation: Philos Trans R Soc Lond B Biol Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: London : Royal Society, 1934- |
| مواضيع طبية MeSH: | Arousal* , Oviposition*, Caenorhabditis elegans/*physiology , Caenorhabditis elegans Proteins/*genetics , Neuropeptides/*genetics , Receptors, G-Protein-Coupled/*genetics, Animals ; Caenorhabditis elegans/genetics ; Caenorhabditis elegans Proteins/metabolism ; Locomotion ; Neuropeptides/metabolism ; Receptors, G-Protein-Coupled/metabolism |
| مستخلص: | Neuropeptide signalling has been implicated in a wide variety of biological processes in diverse organisms, from invertebrates to humans. The Caenorhabditis elegans genome has at least 154 neuropeptide precursor genes, encoding over 300 bioactive peptides. These neuromodulators are thought to largely signal beyond 'wired' chemical/electrical synapse connections, therefore creating a 'wireless' network for neuronal communication. Here, we investigated how behavioural states are affected by neuropeptide signalling through the G protein-coupled receptor SEB-3, which belongs to a bilaterian family of orphan secretin receptors. Using reverse pharmacology, we identified the neuropeptide NLP-49 as a ligand of this evolutionarily conserved neuropeptide receptor. Our findings demonstrate novel roles for NLP-49 and SEB-3 in locomotion, arousal and egg-laying. Specifically, high-content analysis of locomotor behaviour indicates that seb-3 and nlp-49 deletion mutants cause remarkably similar abnormalities in movement dynamics, which are reversed by overexpression of wild-type transgenes. Overexpression of NLP-49 in AVK interneurons leads to heightened locomotor arousal, an effect that is dependent on seb-3. Finally, seb-3 and nlp-49 mutants also show constitutive egg-laying in liquid medium and alter the temporal pattern of egg-laying in similar ways. Together, these results provide in vivo evidence that NLP-49 peptides act through SEB-3 to modulate behaviour, and highlight the importance of neuropeptide signalling in the control of behavioural states.This article is part of a discussion meeting issue 'Connectome to behaviour: modelling C. elegans at cellular resolution'. (© 2018 The Authors.) |
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| معلومات مُعتمدة: | P40 OD010440 United States OD NIH HHS; MC_UP_1102/6 United Kingdom MRC_ Medical Research Council; MC_U105185857 United Kingdom MRC_ Medical Research Council; MC-A023-5PB91 United Kingdom MRC_ Medical Research Council; WT103784MA United Kingdom WT_ Wellcome Trust; United Kingdom WT_ Wellcome Trust; MC-A658-5TY30 United Kingdom MRC_ Medical Research Council |
| فهرسة مساهمة: | Keywords: Caenorhabditis elegans; arousal; egg-laying; locomotion; neuropeptides |
| سلسلة جزيئية: | figshare 10.6084/m9.figshare.c.4208627 |
| المشرفين على المادة: | 0 (Caenorhabditis elegans Proteins) 0 (Neuropeptides) 0 (Receptors, G-Protein-Coupled) 0 (SEB-3 protein, C elegans) 0 (nlp-49 protein, C elegans) |
| تواريخ الأحداث: | Date Created: 20180912 Date Completed: 20190828 Latest Revision: 20240404 |
| رمز التحديث: | 20240404 |
| مُعرف محوري في PubMed: | PMC6158228 |
| DOI: | 10.1098/rstb.2017.0368 |
| PMID: | 30201834 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1471-2970 |
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| DOI: | 10.1098/rstb.2017.0368 |