دورية أكاديمية
Radioimmunoassay for leukotriene E4. Use for determination of total sulfidopeptide-leukotriene release from rat gastric mucosa.
| العنوان: | Radioimmunoassay for leukotriene E4. Use for determination of total sulfidopeptide-leukotriene release from rat gastric mucosa. |
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| المؤلفون: | Hoppe U, Hoppe EM, Peskar BM, Peskar BA |
| المصدر: | FEBS letters [FEBS Lett] 1986 Nov 10; Vol. 208 (1), pp. 26-30. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: John Wiley & Sons Ltd Country of Publication: England NLM ID: 0155157 Publication Model: Print Cited Medium: Print ISSN: 0014-5793 (Print) Linking ISSN: 00145793 NLM ISO Abbreviation: FEBS Lett Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Jan. 2016- : West Sussex : John Wiley & Sons Ltd. Original Publication: Amsterdam, North-Holland on behalf of the Federation of European Biochemical Societies. |
| مواضيع طبية MeSH: | Amino Acids, Sulfur/*metabolism , Gastric Mucosa/*metabolism , SRS-A/*analogs & derivatives, Animals ; In Vitro Techniques ; Leukotriene E4 ; Male ; Masoprocol/pharmacology ; Radioimmunoassay ; Rats ; Rats, Inbred Strains ; SRS-A/analysis |
| مستخلص: | A conjugate of leukotriene (LT) E4 and bovine serum albumin (BSA) was prepared by covalently linking the free amino group of the hapten to the protein using dimethyl pimelindiimidate (DMP) as coupling reagent. Anti-LTE4 antibodies were raised in rabbits immunized with the conjugate. Binding of [3H]LTE4 to the antibodies is inhibited by 50% with 0.63 ng LTE4, while the relative cross-reaction of LTC4 and LTD4 is 46.3% and 12.6%, respectively. Using the radioimmunoassay release of sulfidopeptide-LT (SP-LT) from rat gastric mucosa incubated in vitro was determined after quantitative enzymatic conversion of SP-LT to LTE4. It could be demonstrated that this method is suitable for determination of SP-LT in biological material. |
| المشرفين على المادة: | 0 (Amino Acids, Sulfur) 0 (SRS-A) 75715-89-8 (Leukotriene E4) 7BO8G1BYQU (Masoprocol) |
| تواريخ الأحداث: | Date Created: 19861110 Date Completed: 19861211 Latest Revision: 20190621 |
| رمز التحديث: | 20231215 |
| DOI: | 10.1016/0014-5793(86)81524-1 |
| PMID: | 3021536 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 0014-5793 |
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| DOI: | 10.1016/0014-5793(86)81524-1 |