دورية أكاديمية
DNA microarray analysis using a smartphone to detect the BRCA-1 gene.
| العنوان: | DNA microarray analysis using a smartphone to detect the BRCA-1 gene. |
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| المؤلفون: | Prasad A; Department of Mechanical and Industrial Engineering, Louisiana State University, Baton Rouge, Louisiana 70803, USA. mgartia@lsu.edu., Hasan SMA; Department of Mechanical and Industrial Engineering, Louisiana State University, Baton Rouge, Louisiana 70803, USA. mgartia@lsu.edu., Grouchy S; Department of Mechanical and Industrial Engineering, Louisiana State University, Baton Rouge, Louisiana 70803, USA. mgartia@lsu.edu., Gartia MR; Department of Mechanical and Industrial Engineering, Louisiana State University, Baton Rouge, Louisiana 70803, USA. mgartia@lsu.edu. |
| المصدر: | The Analyst [Analyst] 2018 Dec 17; Vol. 144 (1), pp. 197-205. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Royal Society of Chemistry Country of Publication: England NLM ID: 0372652 Publication Model: Print Cited Medium: Internet ISSN: 1364-5528 (Electronic) Linking ISSN: 00032654 NLM ISO Abbreviation: Analyst Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: Cambridge : Royal Society of Chemistry Original Publication: London : Chemical Society |
| مواضيع طبية MeSH: | Genes, BRCA1* , Smartphone*, DNA Probes/*genetics , Microarray Analysis/*instrumentation , Microscopy, Fluorescence/*instrumentation , Oligodeoxyribonucleotides/*analysis, Gene Expression ; Lab-On-A-Chip Devices ; Limit of Detection ; Microarray Analysis/methods ; Microscopy, Fluorescence/methods ; Nucleic Acid Hybridization ; Oligodeoxyribonucleotides/genetics ; Point-of-Care Testing |
| مستخلص: | DNA microarrays are used to examine changes in gene expression of a large number of genes simultaneously by fluorescent labeling of complementary DNAs (cDNAs). The major bottleneck in implementing microarray technology in resource-limited settings lies in the detection instrument used for generating images of spotted oligonucleotides post-hybridization. While various methods such as a lateral flow assay have been presented to accomplish point-of-care disease detection, there is no simple and effective instrument available to gather spot images maintaining the standard microarray procedures. Nanotechnology based sensors connected with a portable smartphone readout system have the potential to be implemented in microarray technology. Here, we describe a portable fluorescence microarray based imaging system connected to a smartphone for detecting breast cancer gene expression (BRCA-1) from exon 11. This is based on the interactive binding of probe DNA to Cy3-target DNA. A paper-based microfluidics approach was used to demonstrate the DNA hybridization assay. The imaging principles of the assembled device named "FluoroZen" are similar to those of a fluorescence microscope. It uses two light spectrum filters, one to excite the fluorescent dye and the other to capture the emission spectrum. The images were acquired by using CCD cameras from FluoroZen. The smartphone integrated paper microfluidics platform presented here could be translated into clinical settings to perform point-of-care testing. |
| المشرفين على المادة: | 0 (DNA Probes) 0 (Oligodeoxyribonucleotides) |
| تواريخ الأحداث: | Date Created: 20181011 Date Completed: 20190114 Latest Revision: 20190114 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1039/c8an01020j |
| PMID: | 30302482 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1364-5528 |
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| DOI: | 10.1039/c8an01020j |