دورية أكاديمية
أعرض في EDS

A comparative study of endoderm differentiation in humans and chimpanzees.

التفاصيل البيبلوغرافية
العنوان: A comparative study of endoderm differentiation in humans and chimpanzees.
المؤلفون: Blake LE; Department of Human Genetics, University of Chicago, Chicago, IL, USA., Thomas SM; Department of Human Genetics, University of Chicago, Chicago, IL, USA., Blischak JD; Department of Human Genetics, University of Chicago, Chicago, IL, USA., Hsiao CJ; Department of Human Genetics, University of Chicago, Chicago, IL, USA., Chavarria C; Department of Human Genetics, University of Chicago, Chicago, IL, USA., Myrthil M; Department of Human Genetics, University of Chicago, Chicago, IL, USA., Gilad Y; Department of Human Genetics, University of Chicago, Chicago, IL, USA. gilad@uchicago.edu.; Department of Medicine, University of Chicago, Chicago, IL, USA. gilad@uchicago.edu.; Cummings Life Sciences Center, 920 E. 58th Street, CLSC 317, Chicago, IL, 60637, USA. gilad@uchicago.edu., Pavlovic BJ; Department of Human Genetics, University of Chicago, Chicago, IL, USA.
المصدر: Genome biology [Genome Biol] 2018 Oct 15; Vol. 19 (1), pp. 162. Date of Electronic Publication: 2018 Oct 15.
نوع المنشور: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.
اللغة: English
بيانات الدورية: Publisher: BioMed Central Ltd Country of Publication: England NLM ID: 100960660 Publication Model: Electronic Cited Medium: Internet ISSN: 1474-760X (Electronic) Linking ISSN: 14747596 NLM ISO Abbreviation: Genome Biol Subsets: MEDLINE
أسماء مطبوعة: Publication: London, UK : BioMed Central Ltd
Original Publication: London : Genome Biology Ltd., c2000-
مواضيع طبية MeSH: Cell Differentiation*/genetics, Endoderm/*cytology, Animals ; Bayes Theorem ; Female ; Gene Expression Profiling ; Gene Expression Regulation ; Humans ; Induced Pluripotent Stem Cells/cytology ; Induced Pluripotent Stem Cells/metabolism ; Male ; Pan troglodytes ; Primitive Streak/metabolism ; Time Factors
مستخلص: Background: There is substantial interest in the evolutionary forces that shaped the regulatory framework in early human development. Progress in this area has been slow because it is difficult to obtain relevant biological samples. Induced pluripotent stem cells (iPSCs) may provide the ability to establish in vitro models of early human and non-human primate developmental stages.
Results: Using matched iPSC panels from humans and chimpanzees, we comparatively characterize gene regulatory changes through a four-day time course differentiation of iPSCs into primary streak, endoderm progenitors, and definitive endoderm. As might be expected, we find that differentiation stage is the major driver of variation in gene expression levels, followed by species. We identify thousands of differentially expressed genes between humans and chimpanzees in each differentiation stage. Yet, when we consider gene-specific dynamic regulatory trajectories throughout the time course, we find that at least 75% of genes, including nearly all known endoderm developmental markers, have similar trajectories in the two species. Interestingly, we observe a marked reduction of both intra- and inter-species variation in gene expression levels in primitive streak samples compared to the iPSCs, with a recovery of regulatory variation in endoderm progenitors.
Conclusions: The reduction of variation in gene expression levels at a specific developmental stage, paired with overall high degree of conservation of temporal gene regulation, is consistent with the dynamics of a conserved developmental process.
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معلومات مُعتمدة: R01 MH084703 United States MH NIMH NIH HHS; T32 GM007281 United States GM NIGMS NIH HHS; T32 GM07197 United States NH NIH HHS; T32 MH020065 United States MH NIMH NIH HHS; T32MH020065 United States NH NIH HHS; MH084703 United States GM NIGMS NIH HHS; T32 GM007197 United States GM NIGMS NIH HHS
فهرسة مساهمة: Keywords: Comparative genomics; Functional genomics; Gene expression
تواريخ الأحداث: Date Created: 20181017 Date Completed: 20190222 Latest Revision: 20240402
رمز التحديث: 20240402
مُعرف محوري في PubMed: PMC6191992
DOI: 10.1186/s13059-018-1490-5
PMID: 30322406
قاعدة البيانات: MEDLINE
الوصف
تدمد:1474-760X
DOI:10.1186/s13059-018-1490-5