دورية أكاديمية
أعرض في EDS

Rapid differentiation of the human RPE cell line, ARPE-19, induced by nicotinamide.

التفاصيل البيبلوغرافية
العنوان: Rapid differentiation of the human RPE cell line, ARPE-19, induced by nicotinamide.
المؤلفون: Hazim RA; Department of Ophthalmology and Stein Eye Institute, University of California, Los Angeles, CA, USA., Volland S; Department of Ophthalmology and Stein Eye Institute, University of California, Los Angeles, CA, USA., Yen A; Department of Ophthalmology and Stein Eye Institute, University of California, Los Angeles, CA, USA., Burgess BL; Department of Ophthalmology and Stein Eye Institute, University of California, Los Angeles, CA, USA., Williams DS; Department of Ophthalmology and Stein Eye Institute, University of California, Los Angeles, CA, USA; Department of Neurobiology, David Geffen School of Medicine at UCLA, University of California, Los Angeles, CA, USA; Molecular Biology Institute, University of California, Los Angeles, CA, USA; Brain Research Institute, University of California, Los Angeles, CA, USA. Electronic address: dswilliams@ucla.edu.
المصدر: Experimental eye research [Exp Eye Res] 2019 Feb; Vol. 179, pp. 18-24. Date of Electronic Publication: 2018 Oct 15.
نوع المنشور: Journal Article; Research Support, N.I.H., Extramural
اللغة: English
بيانات الدورية: Publisher: Academic Press Country of Publication: England NLM ID: 0370707 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1096-0007 (Electronic) Linking ISSN: 00144835 NLM ISO Abbreviation: Exp Eye Res Subsets: MEDLINE
أسماء مطبوعة: Publication: London : Academic Press
Original Publication: London.
مواضيع طبية MeSH: Cell Differentiation/*drug effects , Niacinamide/*pharmacology , Retinal Pigment Epithelium/*cytology , Vitamin B Complex/*pharmacology, Bestrophins/genetics ; Bestrophins/metabolism ; Biomarkers/metabolism ; Cell Line ; Cytoskeleton/ultrastructure ; Fluorescent Antibody Technique, Indirect ; Gene Expression Regulation/physiology ; Humans ; Integrin beta Chains/genetics ; Integrin beta Chains/metabolism ; Microvilli/ultrastructure ; Occludin/genetics ; Occludin/metabolism ; Real-Time Polymerase Chain Reaction ; Retinal Pigment Epithelium/metabolism ; Zonula Occludens-1 Protein/metabolism ; cis-trans-Isomerases/genetics ; cis-trans-Isomerases/metabolism
مستخلص: Human RPE cell lines, especially the ARPE-19 cell line, are widely-used in eye research, as well as general epithelial cell studies. In comparison with primary RPE cells, they offer relative convenience and consistency, but cultures derived from these lines are typically not well differentiated. We describe a simple, rapid method to establish cultures from ARPE-19 cells, with significantly improved epithelial cell morphology and cytoskeletal organization, and RPE-related functions. We identify the presence of nicotinamide, a member of the vitamin B family, as an essential factor in promoting the observed differentiation, indicating the importance of metabolism in RPE cell differentiation.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
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معلومات مُعتمدة: F31 EY026805 United States EY NEI NIH HHS; P30 EY000331 United States EY NEI NIH HHS; R01 EY013408 United States EY NEI NIH HHS; R01 EY027442 United States EY NEI NIH HHS
المشرفين على المادة: 0 (BEST1 protein, human)
0 (Bestrophins)
0 (Biomarkers)
0 (ITGB5 protein, human)
0 (Integrin beta Chains)
0 (OCLN protein, human)
0 (Occludin)
0 (TJP1 protein, human)
0 (Zonula Occludens-1 Protein)
12001-76-2 (Vitamin B Complex)
25X51I8RD4 (Niacinamide)
EC 3.1.1.64 (retinoid isomerohydrolase)
EC 5.2.- (cis-trans-Isomerases)
تواريخ الأحداث: Date Created: 20181019 Date Completed: 20190415 Latest Revision: 20200309
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC6360117
DOI: 10.1016/j.exer.2018.10.009
PMID: 30336127
قاعدة البيانات: MEDLINE
الوصف
تدمد:1096-0007
DOI:10.1016/j.exer.2018.10.009