دورية أكاديمية
Antifungal potential of punicalagin against Cryptococcus neoformans species complex.
العنوان: | Antifungal potential of punicalagin against Cryptococcus neoformans species complex. |
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المؤلفون: | Silva TC; Universidade Federal de Goiás, Instituto de Patologia Tropical e Saúde Pública, Goiânia, Goiás, Brazil., Zara ALSA; Universidade Federal de Goiás, Instituto de Patologia Tropical e Saúde Pública, Goiânia, Goiás, Brazil., Sá FADS; Universidade Federal de Goiás, Faculdade de Farmácia, Goiânia, Goiás, Brazil., Bara MTF; Universidade Federal de Goiás, Faculdade de Farmácia, Goiânia, Goiás, Brazil., Ávila RI; Universidade Federal de Goiás, Faculdade de Farmácia, Goiânia, Goiás, Brazil., Costa CR; Universidade Federal de Goiás, Instituto de Patologia Tropical e Saúde Pública, Goiânia, Goiás, Brazil., Valadares MC; Universidade Federal de Goiás, Faculdade de Farmácia, Goiânia, Goiás, Brazil., Santos ASD; Universidade Federal de Goiás, Instituto de Patologia Tropical e Saúde Pública, Goiânia, Goiás, Brazil., Freitas VAQ; Universidade Federal de Goiás, Instituto de Patologia Tropical e Saúde Pública, Goiânia, Goiás, Brazil., Silva MDRR; Universidade Federal de Goiás, Instituto de Patologia Tropical e Saúde Pública, Goiânia, Goiás, Brazil. |
المصدر: | Revista do Instituto de Medicina Tropical de Sao Paulo [Rev Inst Med Trop Sao Paulo] 2018 Oct 22; Vol. 60, pp. e60. Date of Electronic Publication: 2018 Oct 22. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo Country of Publication: Brazil NLM ID: 7507484 Publication Model: Electronic Cited Medium: Internet ISSN: 1678-9946 (Electronic) Linking ISSN: 00364665 NLM ISO Abbreviation: Rev Inst Med Trop Sao Paulo Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Sao Paulo : Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo |
مواضيع طبية MeSH: | Antifungal Agents/*pharmacology , Cryptococcus neoformans/*drug effects , Hydrolyzable Tannins/*pharmacology , Lythraceae/*chemistry , Plant Leaves/*chemistry, Animals ; Cryptococcus neoformans/isolation & purification ; Cytotoxicity Tests, Immunologic ; Microbial Sensitivity Tests |
مستخلص: | This study evaluated the antifungal activity and cytotoxicity profile of the ellagitannin punicalagin, a compound extracted from the L. pacari A. St.-Hil (Lythraceae) leaf, against Cryptococcus neoformans species complex. Minimum inhibitory concentrations (MIC) were checked using the broth microdilution method. Minimum fungicidal concentrations (MFC) and time of death were used to confirm the antifungal activity of the compound. The in vitro cytotoxicity of punicalagin was tested in BALB/c3T3 fibroblasts and A549 human lung cancer cell line, while the hemolytic potential was tested on sheep erythrocytes. The morphological changes induced in yeast strains by the presence of punicalagin were also analyzed. Tested on eight isolates of the C. neoformans complex punicalagin showed MIC of 0.5 to 4.0 μg/mL and MFC> 256 μg/mL. Punicalagin also demonstrated a good growth inhibitory activity in time-kill curves, but it was not able to achieve a statistically significant reduction of fungal growth suggesting a fungistatic effect of the compound. In vitro cytotoxicity studies using the two cell lines showed that punicalagin has low activity on these cells and no activity on sheep erythrocytes. Morphological changes were seen in the yeasts strains studied when treated with punicalagin. Therefore, punicalagin is a potential antifungal for important pathogenic yeasts and presents a low cytotoxicity profile associated with no hemolytic effects. |
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المشرفين على المادة: | 0 (Antifungal Agents) 0 (Hydrolyzable Tannins) 65995-63-3 (punicalagin) |
تواريخ الأحداث: | Date Created: 20181027 Date Completed: 20190206 Latest Revision: 20230928 |
رمز التحديث: | 20230928 |
مُعرف محوري في PubMed: | PMC6199124 |
DOI: | 10.1590/S1678-9946201860060 |
PMID: | 30365643 |
قاعدة البيانات: | MEDLINE |
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