دورية أكاديمية
Molecular characterization and phylogenetic analysis of Acanthamoeba isolates in tap water of Beni-Suef, Egypt.
| العنوان: | Molecular characterization and phylogenetic analysis of Acanthamoeba isolates in tap water of Beni-Suef, Egypt. |
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| المؤلفون: | Abd El Wahab WM; Department of Medical Parasitology, College of Medicine, Beni-Suef University, Beni-Suef, Egypt., El-Badry AA; Department of Microbiology-Medical Parasitology Section, College of Medicine, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia., Hamdy DA; Department of Medical Parasitology, College of Medicine, Beni-Suef University, Beni-Suef, Egypt. |
| المصدر: | Acta parasitologica [Acta Parasitol] 2018 Dec 19; Vol. 63 (4), pp. 826-834. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Springer International Publishing Country of Publication: Switzerland NLM ID: 9301947 Publication Model: Print Cited Medium: Internet ISSN: 1896-1851 (Electronic) Linking ISSN: 12302821 NLM ISO Abbreviation: Acta Parasitol Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2019- : Cham : Springer International Publishing Original Publication: Warszawa : Witold Stefanski Institute Of Parasitology |
| مواضيع طبية MeSH: | Phylogeny* , Water Supply*, Acanthamoeba/*classification , Water/*parasitology, Acanthamoeba/genetics ; Acanthamoeba/isolation & purification ; Cross-Sectional Studies ; DNA, Protozoan/chemistry ; DNA, Protozoan/isolation & purification ; DNA, Ribosomal/chemistry ; Egypt ; Hospitals, Private ; Hospitals, Public ; Housing ; Likelihood Functions ; Polymerase Chain Reaction |
| مستخلص: | The genus Acanthamoeba is a free-living amoeba widely distributed in various aquatic environments. It is an etiologic cause of amoebic encephalitis and keratitis particularly for immunocompromised individuals. The purpose of the present study was to investigate Acanthamoeba species prevalence in household and hospital potable water in Beni-Suef governorate, Egypt, and to employ sequencing methods to identify positive Acanthamoeba species isolates and their potential health risks. Sixty tap water samples (30 household and 30 governmental and private hospital settings) collected from Beni-Suef governorate, Egypt were filtered, cultured on non-nutrient agar, identified by morphotyping keys after staining with Giemsa stain and then confirmed by PCR using Acanthamoeba specific primers. Twenty positive samples were successfully genetically characterized and phylogenetically analyzed to identify Acanthamoeba species. The total detection rate for Acanthamoeba was 48/60 (80%); Acanthamoeba contamination in water collected from domestic houses was higher than in hospitals; 27/30 (90%) versus 21/30 (70%) with statistical significant value (P value = 0.05). Sequencing of 20 positive isolates revealed Acanthamoeba T4 in 65% and T2 in 35%. To our knowledge, this is the first research that documents the occurrence and phylogeny of Acanthamoeba species in Beni-Suef, Egypt. The presence of a higher percentage of Acanthamoeba species in tap water, in particular T4, highlights the potential health hazards for immunocompromised individuals and emphasizes the urgent need for the implementation of effective filtration and disinfection measures. |
| فهرسة مساهمة: | Keywords: Acanthamoeba spp.; Egypt; genetic characterization; water resources |
| المشرفين على المادة: | 0 (DNA, Protozoan) 0 (DNA, Ribosomal) 059QF0KO0R (Water) |
| تواريخ الأحداث: | Date Created: 20181028 Date Completed: 20181219 Latest Revision: 20190722 |
| رمز التحديث: | 20240829 |
| DOI: | 10.1515/ap-2018-0101 |
| PMID: | 30367777 |
| قاعدة البيانات: | MEDLINE |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1896-1851 |
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| DOI: | 10.1515/ap-2018-0101 |