دورية أكاديمية
أعرض في EDS

Persistence and Intra-Host Genetic Evolution of Zika Virus Infection in Symptomatic Adults: A Special View in the Male Reproductive System.

التفاصيل البيبلوغرافية
العنوان: Persistence and Intra-Host Genetic Evolution of Zika Virus Infection in Symptomatic Adults: A Special View in the Male Reproductive System.
المؤلفون: Oliveira DBL; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP 05508-000, Brazil. danibruna@gmail.com., Durigon GS; Medical School Clinic Hospital, University of São Paulo, São Paulo, SP 05403-000, Brazil. giuliana.durigon@gmail.com., Mendes ÉA; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP 05508-000, Brazil. ericaarmendes@gmail.com., Ladner JT; Center for Genome Sciences, US Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USA. jtladner@gmail.com.; The Pathogen and Microbiome Institute, Northern Arizona University, Flagstaff, AZ 86011-4073, USA. jtladner@gmail.com., Andreata-Santos R; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP 05508-000, Brazil. robert_andreata@hotmail.com., Araujo DB; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP 05508-000, Brazil. daniellebastos@yahoo.com.br., Botosso VF; Virology Laboratory, Butantan Institute, São Paulo, SP 05503-900, Brazil. viviane.botosso@butantan.gov.br., Paola ND; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP 05508-000, Brazil. nicholasdipaola@gmail.com., Neto DFL; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP 05508-000, Brazil. danielviro@gmail.com., Cunha MP; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP 05508-000, Brazil. marieltondospassos@gmail.com., Braconi CT; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP 05508-000, Brazil. cabraconi@gmail.com., Alves RPS; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP 05508-000, Brazil. rubens.bmc@gmail.com., Jesus MR; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP 05508-000, Brazil. modrigues4@gmail.com., Pereira LR; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP 05508-000, Brazil. lennon_rp@hotmail.com., Melo SR; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP 05508-000, Brazil. stellmelo@gmail.com., Mesquita FS; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP 05508-000, Brazil. flavio.mesquita@usp.br., Silveira VB; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP 05508-000, Brazil. vanessa.silveirabio@gmail.com., Thomazelli LM; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP 05508-000, Brazil. lucmt@usp.br., Favoretto SR; Pasteur Institute, State Health Department, São Paulo, SP 1103-000, Brazil. srfavoretto@usp.br., Almonfrey FB; Medical School Clinic Hospital, University of São Paulo, São Paulo, SP 05403-000, Brazil. fran_almonfrey@hotmail.com., Abdulkader RCRM; Medical School Clinic Hospital, University of São Paulo, São Paulo, SP 05403-000, Brazil. kader@usp.br., Gabrili JM; Virology Laboratory, Butantan Institute, São Paulo, SP 05503-900, Brazil. joel.megalegabrili@gmail.com.; Immunochemistry Laboratory, Butantan Institute, São Paulo, SP 05503-900, Brazil. joel.megalegabrili@gmail.com., Tambourgi DV; Virology Laboratory, Butantan Institute, São Paulo, SP 05503-900, Brazil. denise.tambourgi@butantan.gov.br., Oliveira SF; Department of Cellular and Developmental Biology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP 05508-000, Brazil. sfolivei@gmail.com., Prieto K; Center for Genome Sciences, US Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USA. karla.prieto.ctr@mail.mil.; Department of Environmental, Agricultural and Occupational Health, University of Nebraska Medical Center, Omaha, NE 68198-4388, USA. karla.prieto.ctr@mail.mil., Wiley MR; Center for Genome Sciences, US Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USA. michael.r.wiley19.ctr@mail.mil.; Department of Environmental, Agricultural and Occupational Health, University of Nebraska Medical Center, Omaha, NE 68198-4388, USA. michael.r.wiley19.ctr@mail.mil., Ferreira LCS; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP 05508-000, Brazil. lcsf@usp.br., Silva MV; Institute of Infectology Emílio Ribas e Pontifícia Universidade Católica (PUC-SP), São Paulo, SP 01246-900, Brazil. mvsilva@pucsp.br., Palacios GF; Center for Genome Sciences, US Army Medical Research Institute of Infectious Diseases, Frederick, MD 21702, USA. gustavo.f.palacios.ctr@mail.mil., Zanotto PMA; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP 05508-000, Brazil. pzanotto@usp.br., Durigon EL; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP 05508-000, Brazil. eldurigo@usp.br.
المصدر: Viruses [Viruses] 2018 Nov 07; Vol. 10 (11). Date of Electronic Publication: 2018 Nov 07.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 101509722 Publication Model: Electronic Cited Medium: Internet ISSN: 1999-4915 (Electronic) Linking ISSN: 19994915 NLM ISO Abbreviation: Viruses Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI
مواضيع طبية MeSH: Evolution, Molecular* , Host-Pathogen Interactions*/immunology, Zika Virus/*physiology , Zika Virus Infection/*virology, Brazil/epidemiology ; Cytokines/metabolism ; Female ; Genitalia, Male/virology ; Humans ; Male ; Semen/metabolism ; Semen/virology ; Zika Virus/classification ; Zika Virus/ultrastructure ; Zika Virus Infection/epidemiology ; Zika Virus Infection/immunology ; Zika Virus Infection/transmission
مستخلص: We followed the presence of Zika virus (ZIKV) in four healthy adults (two men and two women), for periods ranging from 78 to 298 days post symptom onset. The patients were evaluated regarding the presence of the virus in different body fluids (blood, saliva, urine and semen), development of immune responses (including antibodies, cytokines and chemokines), and virus genetic variation within samples collected from semen and urine during the infection course. The analysis was focused primarily on the two male patients who shed the virus for up to 158 days after the initial symptoms. ZIKV particles were detected in the spermatozoa cytoplasm and flagella, in immature sperm cells and could also be isolated from semen in cell culture, confirming that the virus is able to preserve integrity and infectivity during replication in the male reproductive system (MRS). Despite the damage caused by ZIKV infection within the MRS, our data showed that ZIKV infection did not result in infertility at least in one of the male patients. This patient was able to conceive a child after the infection. We also detected alterations in the male genital cytokine milieu, which could play an important role in the replication and transmission of the virus which could considerably increase the risk of ZIKV sexual spread. In addition, full genome ZIKV sequences were obtained from several samples (mainly semen), which allowed us to monitor the evolution of the virus within a patient during the infection course. We observed genetic changes over time in consensus sequences and lower frequency intra-host single nucleotide variants (iSNV), that suggested independent compartmentalization of ZIKV populations in the reproductive and urinary systems. Altogether, the present observations confirm the risks associated with the long-term replication and shedding of ZIKV in the MRS and help to elucidate patterns of intra-host genetic evolution during long term replication of the virus.
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فهرسة مساهمة: Keywords: Zika virus; arbovirus; flavivirus; host genetic variation; immune response; sexual transmission
المشرفين على المادة: 0 (Cytokines)
تواريخ الأحداث: Date Created: 20181109 Date Completed: 20190108 Latest Revision: 20190108
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC6267439
DOI: 10.3390/v10110615
PMID: 30405055
قاعدة البيانات: MEDLINE
الوصف
تدمد:1999-4915
DOI:10.3390/v10110615