دورية أكاديمية
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RNA-seq profiling reveals differentially expressed genes as potential markers for vital reaction in skin contusion: a pilot study.

التفاصيل البيبلوغرافية
العنوان: RNA-seq profiling reveals differentially expressed genes as potential markers for vital reaction in skin contusion: a pilot study.
المؤلفون: Xu J; Department of Forensic Pathology, School of Forensic Mecicine, Southern Medical University, Guangzhou, China., Zhao R; Department of Forensic Pathology, China Medical University School of Forensic Medicine, Shenyang, China., Xue Y; Department of Forensic Pathology, School of Forensic Mecicine, Southern Medical University, Guangzhou, China., Xiao H; Department of Pathology, The Third Affiliated Hospital, Sun Yat-Sen University, Guangzhou, China., Sheng Y; Department of Forensic Medicine, School of Basic Medicine, Jiamusi University, Jiamusi, China., Zhao D; CollaborativeInnovation Center of Judicial Civilization, China and Key Laboratory of Evidence Science, China University of Political Science and Law, Ministry of Education, Beijing, China., He J; Department of Forensic Pathology, School of Forensic Mecicine, Southern Medical University, Guangzhou, China., Huang H; Department of Forensic Pathology, School of Forensic Mecicine, Southern Medical University, Guangzhou, China., Wang Q; Department of Forensic Pathology, School of Forensic Mecicine, Southern Medical University, Guangzhou, China., Wang H; Department of Forensic Pathology, School of Forensic Mecicine, Southern Medical University, Guangzhou, China.
المصدر: Forensic sciences research [Forensic Sci Res] 2017 Jul 18; Vol. 3 (2), pp. 153-160. Date of Electronic Publication: 2017 Jul 18 (Print Publication: 2018).
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Oxford University Press Country of Publication: England NLM ID: 101724928 Publication Model: eCollection Cited Medium: Internet ISSN: 2471-1411 (Electronic) Linking ISSN: 24711411 NLM ISO Abbreviation: Forensic Sci Res Subsets: PubMed not MEDLINE
أسماء مطبوعة: Publication: 2023- : [Oxford] : Oxford University Press
Original Publication: [Abingdon, Oxfordshire, United Kingdom] : Taylor & Francis Group, [2016]-
مستخلص: Detection of the vitality of wounds is essential in forensic practice. The present study used Illumina RNA-seq technology to determine gene expression profiles in contused mouse skin. In obtained high quality sequencing reads, the reads were mapped onto a reference transcriptome (Mus_musculus.GRCm38.83). The results revealed that there were 659 up-regulated and 996 down-regulated differentially expressed genes (DEGs) in contused mouse skin. The DEGs were further analyzed using the Gene Ontology and the Kyoto Encyclopedia of Genes and Genomes databases. Genes from different functional categories and signalling pathways were enriched, including the immune system process, immune response, defense response, cytokine-cytokine receptor interaction, complement and coagulation cascades and chemokine signalling pathway. Expression patterns of 11 DEGs were verified by RT-qPCR in mice skins. In addition, alterations of five DEGs were also analyzed in postmortem human wound samples. The results were in concordance with the results of RNA-seq. These findings suggest that RNA-seq is a powerful tool to reveal DEGs as potential markers for vital reaction in terms of forensic practices.
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فهرسة مساهمة: Keywords: Forensic pathology; RNA-seq; differentially expressed genes; skin contusion; vital reaction
تواريخ الأحداث: Date Created: 20181129 Latest Revision: 20200930
رمز التحديث: 20240829
مُعرف محوري في PubMed: PMC6197083
DOI: 10.1080/20961790.2017.1349639
PMID: 30483664
قاعدة البيانات: MEDLINE
الوصف
تدمد:2471-1411
DOI:10.1080/20961790.2017.1349639