دورية أكاديمية
أعرض في EDS

BE-FLARE: a fluorescent reporter of base editing activity reveals editing characteristics of APOBEC3A and APOBEC3B.

التفاصيل البيبلوغرافية
العنوان: BE-FLARE: a fluorescent reporter of base editing activity reveals editing characteristics of APOBEC3A and APOBEC3B.
المؤلفون: Coelho MA; Discovery Sciences, IMED Biotech Unit, AstraZeneca, Cambridge, UK. Matthew.Coelho@astrazeneca.com., Li S; Discovery Sciences, IMED Biotech Unit, AstraZeneca, Gothenburg, Sweden., Pane LS; Discovery Sciences, IMED Biotech Unit, AstraZeneca, Gothenburg, Sweden., Firth M; Discovery Sciences, IMED Biotech Unit, AstraZeneca, Cambridge, UK., Ciotta G; Discovery Sciences, IMED Biotech Unit, AstraZeneca, Cambridge, UK., Wrigley JD; Discovery Sciences, IMED Biotech Unit, AstraZeneca, Cambridge, UK., Cuomo ME; Discovery Sciences, IMED Biotech Unit, AstraZeneca, Cambridge, UK., Maresca M; Discovery Sciences, IMED Biotech Unit, AstraZeneca, Gothenburg, Sweden., Taylor BJM; Discovery Sciences, IMED Biotech Unit, AstraZeneca, Cambridge, UK. Benjamin.Taylor@astrazeneca.com.
المصدر: BMC biology [BMC Biol] 2018 Dec 28; Vol. 16 (1), pp. 150. Date of Electronic Publication: 2018 Dec 28.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: BioMed Central Country of Publication: England NLM ID: 101190720 Publication Model: Electronic Cited Medium: Internet ISSN: 1741-7007 (Electronic) Linking ISSN: 17417007 NLM ISO Abbreviation: BMC Biol Subsets: MEDLINE
أسماء مطبوعة: Original Publication: [London] : BioMed Central, c2003-
مواضيع طبية MeSH: APOBEC-1 Deaminase/*genetics , Cytidine Deaminase/*genetics , Gene Editing/*methods , Genetic Engineering/*methods , Minor Histocompatibility Antigens/*genetics , Proteins/*genetics, Animals ; Humans ; Rats
مستخلص: Background: Base Editing is a precise genome editing method that uses a deaminase-Cas9 fusion protein to mutate cytidine to thymidine in target DNA in situ without the generation of a double-strand break. However, the efficient enrichment of genetically modified cells using this technique is limited by the ability to detect such events.
Results: We have developed a Base Editing FLuorescent Activity REporter (BE-FLARE), which allows for the enrichment of cells that have undergone editing of target loci based on a fluorescence shift from BFP to GFP. We used BE-FLARE to evaluate the editing efficiency of APOBEC3A and APOBEC3B family members as alternatives deaminase domains to the rat APOBEC1 domain used in base editor 3 (BE3). We identified human APOBEC3A and APOBEC3B as highly efficient cytidine deaminases for base editing applications with unique properties.
Conclusions: Using BE-FLARE to report on the efficiency and precision of editing events, we outline workflows for the accelerated generation of genetically engineered cell models and the discovery of alternative base editors.
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فهرسة مساهمة: Keywords: APOBEC; Base editing; CRISPR/Cas9; Fluorescent reporter; Gene editing
المشرفين على المادة: 0 (Minor Histocompatibility Antigens)
0 (Proteins)
EC 3.5.4.36 (APOBEC-1 Deaminase)
EC 3.5.4.36 (Apobec1 protein, rat)
EC 3.5.4.5 (APOBEC3A protein, human)
EC 3.5.4.5 (APOBEC3B protein, human)
EC 3.5.4.5 (Cytidine Deaminase)
تواريخ الأحداث: Date Created: 20181230 Date Completed: 20190503 Latest Revision: 20231005
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC6309101
DOI: 10.1186/s12915-018-0617-1
PMID: 30593278
قاعدة البيانات: MEDLINE
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