دورية أكاديمية
Conjugation of carbon coated-iron nanoparticles with biomolecules for NMR-based assay.
| العنوان: | Conjugation of carbon coated-iron nanoparticles with biomolecules for NMR-based assay. |
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| المؤلفون: | Khramtsov P; Laboratory of Ecological Immunology, 'Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences' - Branch of PSRC UB RAS, 614081, 13 Golev Str., Perm, Russia; Department of Microbiology and Immunology, Biology Faculty, Perm State National Research University, 614000, 15 Bukirev Str., Perm, Russia. Electronic address: khramtsovpavel@yandex.ru., Kropaneva M; Laboratory of Ecological Immunology, 'Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences' - Branch of PSRC UB RAS, 614081, 13 Golev Str., Perm, Russia., Byzov I; Laboratory of Ecological Immunology, 'Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences' - Branch of PSRC UB RAS, 614081, 13 Golev Str., Perm, Russia; M.N. Mikheev Institute of Metal Physics of the Ural Branch of the Russian Academy of Sciences, 620108, 18 S. Kovalevskoy Str., Ekaterinburg, Russia., Minin A; Laboratory of Ecological Immunology, 'Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences' - Branch of PSRC UB RAS, 614081, 13 Golev Str., Perm, Russia; M.N. Mikheev Institute of Metal Physics of the Ural Branch of the Russian Academy of Sciences, 620108, 18 S. Kovalevskoy Str., Ekaterinburg, Russia; Ural Federal University named after the first President of Russia B.N. Yeltsin, 620002, 19 Mira Str., Ekaterinburg, Russia., Mysik A; M.N. Mikheev Institute of Metal Physics of the Ural Branch of the Russian Academy of Sciences, 620108, 18 S. Kovalevskoy Str., Ekaterinburg, Russia., Timganova V; Laboratory of Ecological Immunology, 'Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences' - Branch of PSRC UB RAS, 614081, 13 Golev Str., Perm, Russia., Bochkova M; Laboratory of Ecological Immunology, 'Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences' - Branch of PSRC UB RAS, 614081, 13 Golev Str., Perm, Russia., Uimin M; Laboratory of Ecological Immunology, 'Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences' - Branch of PSRC UB RAS, 614081, 13 Golev Str., Perm, Russia; M.N. Mikheev Institute of Metal Physics of the Ural Branch of the Russian Academy of Sciences, 620108, 18 S. Kovalevskoy Str., Ekaterinburg, Russia; Ural Federal University named after the first President of Russia B.N. Yeltsin, 620002, 19 Mira Str., Ekaterinburg, Russia., Zamorina S; Laboratory of Ecological Immunology, 'Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences' - Branch of PSRC UB RAS, 614081, 13 Golev Str., Perm, Russia; Department of Microbiology and Immunology, Biology Faculty, Perm State National Research University, 614000, 15 Bukirev Str., Perm, Russia., Yermakov A; Laboratory of Ecological Immunology, 'Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences' - Branch of PSRC UB RAS, 614081, 13 Golev Str., Perm, Russia; M.N. Mikheev Institute of Metal Physics of the Ural Branch of the Russian Academy of Sciences, 620108, 18 S. Kovalevskoy Str., Ekaterinburg, Russia; Ural Federal University named after the first President of Russia B.N. Yeltsin, 620002, 19 Mira Str., Ekaterinburg, Russia., Rayev M; Laboratory of Ecological Immunology, 'Institute of Ecology and Genetics of Microorganisms of the Ural Branch of the Russian Academy of Sciences' - Branch of PSRC UB RAS, 614081, 13 Golev Str., Perm, Russia; Department of Microbiology and Immunology, Biology Faculty, Perm State National Research University, 614000, 15 Bukirev Str., Perm, Russia; Ural Federal University named after the first President of Russia B.N. Yeltsin, 620002, 19 Mira Str., Ekaterinburg, Russia. |
| المصدر: | Colloids and surfaces. B, Biointerfaces [Colloids Surf B Biointerfaces] 2019 Apr 01; Vol. 176, pp. 256-264. Date of Electronic Publication: 2019 Jan 03. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 9315133 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-4367 (Electronic) Linking ISSN: 09277765 NLM ISO Abbreviation: Colloids Surf B Biointerfaces |
| أسماء مطبوعة: | Original Publication: Amsterdam ; New York : Elsevier, c1993- |
| مواضيع طبية MeSH: | Carbon/*chemistry , Iron/*chemistry , Magnetic Resonance Spectroscopy/*methods , Serum Albumin, Bovine/*chemistry, Animals ; Cattle ; Hydrogen-Ion Concentration ; Nanoparticles/chemistry ; Nanoparticles/ultrastructure ; Particle Size ; Reproducibility of Results ; X-Ray Diffraction |
| مستخلص: | In this work, we developed and optimized conjugates of carbon-coated iron nanoparticles (Fe@C) with streptavidin and monoclonal antibodies. The conjugation procedure included two stages. First, amino groups were grafted onto the carbon shell to facilitate noncovalent sorption of bovine serum albumin (BSA). Further, the covalent attachment of proteins to the BSA layer via glutaraldehyde coupling was performed. It was established and confirmed that the synthesis procedure is reproducible and allows preparation of stable conjugates. The resulting nanoparticles are clusters of Fe@C particles coated by proteins. The size of the clusters is in the range of 100-190 nm and can be controlled via the tuning of conjugation conditions, including pH, BSA-to-Fe@C ratio, etc. Conjugates of Fe@C with streptavidin and monoclonal antibodies (sizes of approximately 140-150 nm) were synthesized. Proton T (Copyright © 2019 Elsevier B.V. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Assay; Carbon-coated magnetic nanoparticles; Conjugate; Nuclear magnetic resonance |
| المشرفين على المادة: | 27432CM55Q (Serum Albumin, Bovine) 7440-44-0 (Carbon) E1UOL152H7 (Iron) |
| تواريخ الأحداث: | Date Created: 20190110 Date Completed: 20190801 Latest Revision: 20190801 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1016/j.colsurfb.2019.01.009 |
| PMID: | 30623813 |
| قاعدة البيانات: | MEDLINE |
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