دورية أكاديمية
أعرض في EDS

Antioxidant-antibacterial containing bi-layer scaffolds as potential candidates for management of oxidative stress and infections in wound healing.

التفاصيل البيبلوغرافية
العنوان: Antioxidant-antibacterial containing bi-layer scaffolds as potential candidates for management of oxidative stress and infections in wound healing.
المؤلفون: Shaik MM; Department of Biological Sciences, Birla Institute of Technology and Science Pilani, K K Birla Goa Campus, Goa, 403726, India., Dapkekar A; Nanobioscience group, Agharkar Research Institute, G. G. Agarkar Road, Pune, 411 004, India., Rajwade JM; Nanobioscience group, Agharkar Research Institute, G. G. Agarkar Road, Pune, 411 004, India., Jadhav SH; Animal Sciences Division, Agharkar Research Institute, G. G. Agarkar Road, Pune, 411 004, India., Kowshik M; Department of Biological Sciences, Birla Institute of Technology and Science Pilani, K K Birla Goa Campus, Goa, 403726, India. meenal@goa.bits-pilani.ac.in.
المصدر: Journal of materials science. Materials in medicine [J Mater Sci Mater Med] 2019 Jan 11; Vol. 30 (1), pp. 13. Date of Electronic Publication: 2019 Jan 11.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Springer Country of Publication: United States NLM ID: 9013087 Publication Model: Electronic Cited Medium: Internet ISSN: 1573-4838 (Electronic) Linking ISSN: 09574530 NLM ISO Abbreviation: J Mater Sci Mater Med Subsets: MEDLINE
أسماء مطبوعة: Publication: <2008->: Norwell, MA : Springer
Original Publication: London : Chapman and Hall, c1990-
مواضيع طبية MeSH: Tissue Scaffolds*, Anti-Bacterial Agents/*pharmacology , Antioxidants/*pharmacology , Tissue Engineering/*methods , Wound Healing/*drug effects, Animals ; Anti-Bacterial Agents/administration & dosage ; Antioxidants/administration & dosage ; Bacterial Infections/prevention & control ; COS Cells ; Chlorocebus aethiops ; Lipid Peroxidation ; Oxidative Stress/drug effects ; Random Allocation ; Rats ; Rats, Wistar
مستخلص: Tissue engineering techniques are continuously evolving towards providing better microenvironment along with therapeutic potential to address the skin tissue defects. Factors such as microbial infections, presence of excessive free radicals and depletion in antioxidant based scavenging systems pose serious challenges by prolonging inflammation and delaying the repair process. Incorporation of bioactive molecules in polymer based biomimetic scaffolds may present new vistas for handling chronic wounds. In this study, chitosan/collagen scaffolds incorporating 0.5, 1 and 2% (w/w) silymarin (CS-CO-SM) were synthesized and studied for their biocompatibility, in vitro release kinetics and anti-oxidant activity. The release kinetics of silymarin from the CS-CO-SM scaffold showed an initial burst followed by sustained release. The scaffolds were biocompatible and supported the recovery of COS-7 cells from UV induced oxidative stress. Further the CS-CO-SM (2) scaffolds were used to fabricate a bi-layer scaffold by layer upon layer arrangement with CS-Ag3 (3% Ag, w/w). The Ag was incorporated to impart antimicrobial property to the scaffold. The in vivo studies on bi-layer scaffolds were carried out in Wistar rat models at 3, 7 and 10 days post injury and the skin excisions were studied for wound contraction, histology (H&E staining), and lipid peroxidation. The bi-layer scaffold accelerated the process of wound healing with no inflammatory cells, proliferation of fibroblast, neovascularization and collagen deposition. By day 10 post transplantation of the scaffold, the skin had a structure similar to normal skin with complete re-epithelization. This bi-layer scaffold with antioxidant and antimicrobial properties promotes wound healing and is proposed as a potential tissue engineering material for managing chronic wounds.
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المشرفين على المادة: 0 (Anti-Bacterial Agents)
0 (Antioxidants)
تواريخ الأحداث: Date Created: 20190113 Date Completed: 20190426 Latest Revision: 20200225
رمز التحديث: 20221213
DOI: 10.1007/s10856-018-6212-8
PMID: 30635734
قاعدة البيانات: MEDLINE
الوصف
تدمد:1573-4838
DOI:10.1007/s10856-018-6212-8