دورية أكاديمية
Induction of T cell-mediated immune response by dendritic cells pulsed with mRNA of sphere-forming cells isolated from patients with gastric cancer.
| العنوان: | Induction of T cell-mediated immune response by dendritic cells pulsed with mRNA of sphere-forming cells isolated from patients with gastric cancer. |
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| المؤلفون: | Bagheri V; Cellular and Molecular Research Center, Birjand University of Medical Sciences, Birjand, Iran., Abbaszadegan MR; Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: AbbaszadeganMR@mums.ac.ir., Memar B; Surgical Oncology Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran., Motie MR; Surgical Oncology Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran., Asadi M; Surgical Oncology Research Center, Imam Reza Hospital, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran., Mahmoudian RA; Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran., Gholamin M; Medical Genetics Research Center, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Laboratory Sciences, School of Paramedical Sciences, Mashhad University of Medical Sciences, Mashhad, Iran. Electronic address: GholaminM@mums.ac.ir. |
| المصدر: | Life sciences [Life Sci] 2019 Feb 15; Vol. 219, pp. 136-143. Date of Electronic Publication: 2019 Jan 12. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Elsevier Country of Publication: Netherlands NLM ID: 0375521 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1879-0631 (Electronic) Linking ISSN: 00243205 NLM ISO Abbreviation: Life Sci Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2008->: Amsterdam : Elsevier Original Publication: Oxford; Elmsford, N. Y. [etc.] Pergamon Press. |
| مواضيع طبية MeSH: | Dendritic Cells/*metabolism , Immunity, Cellular/*immunology , Neoplastic Stem Cells/*metabolism , RNA, Messenger/*metabolism , Stomach Neoplasms/*immunology , T-Lymphocytes/*immunology, Animals ; Electroporation ; Flow Cytometry ; Humans ; Immunotherapy/methods ; Interferon-gamma/metabolism ; Male ; Mice, Inbred C57BL ; Neoplasm Transplantation ; Stomach Neoplasms/therapy |
| مستخلص: | Gastric cancer (GC) as the third most common cause of cancer-associated mortality worldwide is one of the cancers with very high heterogeneity. Cancer stem cells (CSCs) as a small subset of cancer cells in solid tumors with the self-renewal, differentiation and tumorigenic ability are responsible for tumor initiation, progression, recurrence, metastasis, and resistance to current treatments. Therefore, eradication of CSCs is very vital to cure cancer. Here, we first isolated and identified sphere-forming cells in tumor tissue from four GC patients and then analyzed T cell responses induced by monocyte-derived dendritic cells (DCs) loaded with total mRNA of sphere-forming cells in terms of interferon-gamma (IFN-γ) gene expression and specific cytotoxicity. Spheroid colonies were formed in serum-free media. Sphere-forming cells dissociated from tumorspheres heterogeneously expressed CD44, CD54, and epithelial cell adhesion molecule (EpCAM) markers and generated one tumor in nude mice. These results demonstrated that gastric CSCs were enriched in tumorspheres. Cytokine-matured DCs loaded with mRNA of sphere-forming cells were able to induce IFN-γ gene expression in T-lymphocytes after a 12-day co-culture. mRNA level of IFN-γ gene in these lymphocytes was more highly expressed compared to stimulated T-lymphocytes by DCs transfected with normal tissue (6.4-9.39 folds). Cytotoxic activity of primed T-lymphocytes with antigens of sphere-forming cells was significantly higher than normal tissue antigens and mock DCs (P ≤ 0.0001). Taken together, DCs loaded with mRNA of sphere-forming cells that elicit effectively specific T cell-mediated immune responses in vitro, may be considered as a promising therapeutic vaccination in GC patients in future. (Copyright © 2019 Elsevier Inc. All rights reserved.) |
| فهرسة مساهمة: | Keywords: Cancer stem cells; Cytotoxicity; Dendritic cells; Sphere-forming cells; T cells |
| المشرفين على المادة: | 0 (RNA, Messenger) 82115-62-6 (Interferon-gamma) |
| تواريخ الأحداث: | Date Created: 20190115 Date Completed: 20190225 Latest Revision: 20190225 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1016/j.lfs.2019.01.016 |
| PMID: | 30641083 |
| قاعدة البيانات: | MEDLINE |
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