دورية أكاديمية
DNA damage in dental pulp mesenchymal stem cells: An in vitro study.
| العنوان: | DNA damage in dental pulp mesenchymal stem cells: An in vitro study. |
|---|---|
| المؤلفون: | Aramburú Junior JS; Graduate Program in Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Brazil.; Biotecnos Research Center, Santa Maria, Rio Grande do Sul, Brazil; Catholic University of Uruguay, Montevideo, Uruguay., Eilers Treichel TL; Graduate Program in Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Brazil., Lemos Pinto Filho ST; Graduate Program in Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Brazil., Gehrke SA; Biotecnos Research Center, Santa Maria, Rio Grande do Sul, Brazil; Catholic University of Uruguay, Montevideo, Uruguay., Machado AK; Morphology Department, Federal University of Santa Maria, Santa Maria, Brazil., Cadoná FC; Morphology Department, Federal University of Santa Maria, Santa Maria, Brazil., Mânica da Cruz IB; Morphology Department, Federal University of Santa Maria, Santa Maria, Brazil., Pippi NL; Graduate Program in Veterinary Medicine, Federal University of Santa Maria, Santa Maria, Brazil. |
| المصدر: | Veterinary research forum : an international quarterly journal [Vet Res Forum] 2018 Fall; Vol. 9 (4), pp. 293-299. Date of Electronic Publication: 2018 Dec 15. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Urmia University Press Country of Publication: Iran NLM ID: 101625812 Publication Model: Print-Electronic Cited Medium: Print ISSN: 2008-8140 (Print) Linking ISSN: 20088140 NLM ISO Abbreviation: Vet Res Forum Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Urmia : Urmia University Press |
| مستخلص: | The aim of this study was to evaluate the potential use of a DNA comet assay, DNA fragmentation fluorimetric assay and reactive oxygen species levels as potential biomarkers of genome conditions of dental pulp stem cells (DPSCs) isolated from dog canine teeth. Mesenchymal stem cells were isolated from the dental pulp collected from dog teeth. The results obtained suggest the ideal moment for clinical application of cellular therapy for this type of cell. The cell culture was maintained with Dulbecco's modified Eagle's medium supplemented with 10.00% fetal bovine serum for eight passages. During each passage, cell proliferation, oxidative stress and level of DNA fragmentation were assessed by3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyltetrazolium (MTT) assay, testing 2,7 dichlorodihydro-fluorescein-diacetate and PicoGreen ® , respectively. There were important differences among the first three DPSC passages compared to passages 4-8 and a large number of nuclei with some levels of DNA damage (30.00 to 40.00% in initial DPSC passages and > 50.00% in late passages), indicating in vitro DPSC genomic fragility. Within the limitations of this study, the results suggest these relatively simple and inexpensive approaches - comet and DNA fragmentation assays - could help sort stem cells with less DNA damage for use in research or therapies. Competing Interests: The authors declare that there is no conflicts of interest. |
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| فهرسة مساهمة: | Keywords: DNA damage; Dental pulp; Genotoxicity; Mesenchymal stem cell; Oxidative stress |
| تواريخ الأحداث: | Date Created: 20190205 Latest Revision: 20201001 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC6346493 |
| DOI: | 10.30466/vrf.2018.33083 |
| PMID: | 30713606 |
| قاعدة البيانات: | MEDLINE |
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