دورية أكاديمية
أعرض في EDS

Effect of tannic acid on the structure and activity of Kluyveromyces lactis β-galactosidase.

التفاصيل البيبلوغرافية
العنوان: Effect of tannic acid on the structure and activity of Kluyveromyces lactis β-galactosidase.
المؤلفون: Kayukawa CTM; Department of Chemical Engineering, Federal University of Paraná (UFPR), Polytechnic Center, Jardim das Américas, 81531-990 Curitiba, PR, Brazil., de Oliveira MAS; Department of Biochemistry, University of Maringá (UEM), 87020-900 Maringá, PR, Brazil., Kaspchak E; Department of Chemical Engineering, Federal University of Paraná (UFPR), Polytechnic Center, Jardim das Américas, 81531-990 Curitiba, PR, Brazil., Sanchuki HBS; Department of Biochemistry and Molecular Biology, Federal University of Paraná (UFPR), Polytechnic Center, Jardim das Américas, 81531-990 Curitiba, PR, Brazil., Igarashi-Mafra L; Department of Chemical Engineering, Federal University of Paraná (UFPR), Polytechnic Center, Jardim das Américas, 81531-990 Curitiba, PR, Brazil., Mafra MR; Department of Chemical Engineering, Federal University of Paraná (UFPR), Polytechnic Center, Jardim das Américas, 81531-990 Curitiba, PR, Brazil. Electronic address: marcos.mafra@ufpr.br.
المصدر: Food chemistry [Food Chem] 2019 Mar 01; Vol. 275, pp. 346-353. Date of Electronic Publication: 2018 Sep 19.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: Elsevier Applied Science Publishers Country of Publication: England NLM ID: 7702639 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-7072 (Electronic) Linking ISSN: 03088146 NLM ISO Abbreviation: Food Chem Subsets: MEDLINE
أسماء مطبوعة: Publication: Barking : Elsevier Applied Science Publishers
Original Publication: Barking, Eng., Applied Science Publishers.
مواضيع طبية MeSH: Kluyveromyces/*enzymology , Tannins/*metabolism , beta-Galactosidase/*chemistry , beta-Galactosidase/*metabolism, Animals ; Calorimetry/methods ; Circular Dichroism ; Fungal Proteins/chemistry ; Fungal Proteins/metabolism ; Humans ; Kinetics ; Nitrophenylgalactosides/chemistry ; Nitrophenylgalactosides/metabolism ; Protein Conformation ; Spectrometry, Fluorescence ; Tannins/chemistry ; Thermodynamics
مستخلص: Tannins are compounds with antinutrient properties that hinder food digestibility, prejudicing human and animal nutrition. This work aimed to evaluate the negative effects of tannic acid on Kluyveromyces lactis β-galactosidase catalytic activity and correlate these changes with the protein structure. β-Galactosidase activity decreased in the presence of tannins, which caused changes to the structure of the enzyme, as demonstrated by circular dichroism. It was verified that tannin binds to the protein by a static mechanism. Additionally, isothermal titration calorimetry suggested that tannic acid modified the molecular interaction between β-galactosidase and o-nitrophenyl-β-d-galactoside, reducing their affinity and prejudicing the protein activity. This study helps to understand the effects of tannins on the β-galactosidase structure and how they are related to the enzyme catalytic activity. The alterations in the conformation and activity of the enzyme should be taken into consideration when dairy products are consumed with tannin-rich food.
(Copyright © 2018 Elsevier Ltd. All rights reserved.)
فهرسة مساهمة: Keywords: Circular dichroism; Dairy products; Fluorescence; Isothermal titration calorimetry; Thermodynamics
المشرفين على المادة: 0 (Fungal Proteins)
0 (Tannins)
19710-96-4 (2-nitrophenylgalactoside)
28347-45-7 (Nitrophenylgalactosides)
EC 3.2.1.23 (beta-Galactosidase)
تواريخ الأحداث: Date Created: 20190207 Date Completed: 20190325 Latest Revision: 20190325
رمز التحديث: 20240628
DOI: 10.1016/j.foodchem.2018.09.107
PMID: 30724206
قاعدة البيانات: MEDLINE
الوصف
تدمد:1873-7072
DOI:10.1016/j.foodchem.2018.09.107