دورية أكاديمية
Semi-quantitative duplex RT-PCR reveals the low occurrence of Porcine Pegivirus and Atypical Porcine Pestivirus in diagnostic samples from the United States.
العنوان: | Semi-quantitative duplex RT-PCR reveals the low occurrence of Porcine Pegivirus and Atypical Porcine Pestivirus in diagnostic samples from the United States. |
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المؤلفون: | Chen F; Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota.; State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China., Knutson TP; Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota., Braun E; Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota., Jiang Y; Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota., Rossow S; Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota., Marthaler DG; Department of Veterinary Population Medicine, College of Veterinary Medicine, University of Minnesota, Saint Paul, Minnesota.; Veterinary Diagnostic Laboratory, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas. |
المصدر: | Transboundary and emerging diseases [Transbound Emerg Dis] 2019 May; Vol. 66 (3), pp. 1420-1425. Date of Electronic Publication: 2019 Mar 21. |
نوع المنشور: | Journal Article |
اللغة: | English |
بيانات الدورية: | Publisher: Blackwell Verlag Country of Publication: Germany NLM ID: 101319538 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1865-1682 (Electronic) Linking ISSN: 18651674 NLM ISO Abbreviation: Transbound Emerg Dis Subsets: MEDLINE |
أسماء مطبوعة: | Original Publication: Berlin : Blackwell Verlag |
مواضيع طبية MeSH: | Metagenomics*, Coinfection/*veterinary , Flaviviridae/*isolation & purification , Flaviviridae Infections/*veterinary , Swine Diseases/*epidemiology, Animals ; Flaviviridae/genetics ; Flaviviridae Infections/epidemiology ; Flaviviridae Infections/virology ; Minnesota/epidemiology ; Pestivirus/genetics ; Pestivirus/isolation & purification ; Pestivirus Infections/epidemiology ; Pestivirus Infections/veterinary ; Pestivirus Infections/virology ; Phylogeny ; Reverse Transcriptase Polymerase Chain Reaction/veterinary ; Swine ; Swine Diseases/virology ; United States/epidemiology |
مستخلص: | Porcine Pegivirus (PPgV) and Atypical Porcine Pestivirus (APPV) are two recently identified porcine viruses. In this study, the identification of two viruses by metagenomic sequencing, and a duplex semi-quantitative RT-PCR was developed to detect these pathogens simultaneously. The PPgV strain Minnesota-1/2016 had a 95.5%-96.3% nucleotide identity and clustered with the recently identified US PPgV strains, which is a distant clade from the German PPgV strains. The APPV strain Minnesota-1/2016 shared an 87.3%-92.0% nucleotide identity with the other global APPV strains identity but only shared an 82.8%-83.0% nucleotide identity with clade II consisting of strain identified in China. Detection of both PPgV and APPV was 9.0% of the diagnostic cases. Co-infection of PPgV and APPV was identified in 7.5% of the diagnostic cases. The occurrence and genetic characterization of PPgV and APPV further enhance our knowledge regarding these new pathogens in the United States. (© 2019 The Authors. Transboundary and Emerging Diseases published by Blackwell Verlag GmbH.) |
References: | Acta Trop. 2015 Dec;152:56-59. (PMID: 26314230) Transbound Emerg Dis. 2017 Aug;64(4):1020-1023. (PMID: 28497656) Sci Rep. 2016 Jun 13;6:27735. (PMID: 27292119) mSphere. 2018 Aug 22;3(4):. (PMID: 30135221) J Gen Virol. 2015 Oct;96(10):2994-2998. (PMID: 26219947) Genome Biol Evol. 2015 Oct 21;7(11):2996-3008. (PMID: 26494702) J Med Virol. 2019 Jan;91(1):38-44. (PMID: 30133782) PLoS Pathog. 2015 Dec 11;11(12):e1005325. (PMID: 26658760) Emerg Infect Dis. 2018 May;24(5):908-911. (PMID: 29664364) Transbound Emerg Dis. 2019 May;66(3):1420-1425. (PMID: 30806022) Front Microbiol. 2017 May 11;8:862. (PMID: 28553280) J Gen Virol. 2017 Jun;98(6):1305-1310. (PMID: 28590234) J Virol. 2016 Jul 11;90(15):6724-6737. (PMID: 27170760) Vet Rec. 2017 Jan 7;180(1):14-15. (PMID: 28062774) Sci Rep. 2018 Mar 22;8(1):5036. (PMID: 29568043) Prev Vet Med. 2016 Jan 1;123:175-184. (PMID: 26611651) Can Vet J. 1991 Jun;32(6):362-4. (PMID: 17423804) Viruses. 2016 Oct 04;8(10):. (PMID: 27782037) J Gen Virol. 2018 Jun;99(6):768-776. (PMID: 29658859) Br J Haematol. 2018 Sep;182(5):644-653. (PMID: 29808922) Expert Rev Anti Infect Ther. 2012 May;10(5):563-72. (PMID: 22702320) Emerg Infect Dis. 2016 Jul;22(7):1312-4. (PMID: 27314228) J Virol. 2013 Jun;87(12):7185-90. (PMID: 23596285) Transbound Emerg Dis. 2017 Dec;64(6):1645-1649. (PMID: 28941140) Transbound Emerg Dis. 2018 Dec;65(6):2039-2042. (PMID: 30105779) Viruses. 2018 Feb 28;10(3):. (PMID: 29495551) Transbound Emerg Dis. 2018 Feb;65(1):e202-e204. (PMID: 28710801) Transbound Emerg Dis. 2019 Jan;66(1):35-42. (PMID: 30281923) Transbound Emerg Dis. 2018 Jun;65(3):618-626. (PMID: 29484834) Can Vet J. 2018 Apr;59(4):429-432. (PMID: 29606732) J Med Virol. 2018 Dec;90(12):1814-1821. (PMID: 30011073) PLoS One. 2016 Feb 24;11(2):e0150104. (PMID: 26909691) Transbound Emerg Dis. 2018 Apr;65(2):375-380. (PMID: 29393592) J Vet Sci. 2018 May 31;19(3):468-471. (PMID: 29284212) J Gen Virol. 2016 Nov;97(11):2894-2907. (PMID: 27692039) Virus Genes. 2018 Jun;54(3):361-367. (PMID: 29551002) |
معلومات مُعتمدة: | CARS-36 China Agriculture Research System; 201606760012 China Scholarship Council; Hatch 1013569 National Institute of Food and Agriculture |
فهرسة مساهمة: | Keywords: Pegivirus; atypical porcine pestivirus; phylogenetics |
سلسلة جزيئية: | GENBANK KU351669; KU351671; KY798013; KY624591; KX929062; KX778724; KU041639; KY612413; KX950762; KX950761; KY652092; LT594521; KU194229; KR011347; MF590069 |
تواريخ الأحداث: | Date Created: 20190227 Date Completed: 20190830 Latest Revision: 20210109 |
رمز التحديث: | 20231215 |
مُعرف محوري في PubMed: | PMC6849716 |
DOI: | 10.1111/tbed.13154 |
PMID: | 30806022 |
قاعدة البيانات: | MEDLINE |
تدمد: | 1865-1682 |
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DOI: | 10.1111/tbed.13154 |