دورية أكاديمية
Microbiome diversity in carriers of fluoroquinolone resistant Escherichia coli .
| العنوان: | Microbiome diversity in carriers of fluoroquinolone resistant Escherichia coli . |
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| المؤلفون: | Liss MA; Department of Urology, UT Health San Antonio Long School of Medicine, San Antonio, TX, USA.; South Texas Veterans Healthcare System, San Antonio, TX, USA., Leach RJ; Department of Urology, UT Health San Antonio Long School of Medicine, San Antonio, TX, USA.; Department of Cell Systems and Anatomy, UT Health San Antonio Long School of Medicine, San Antonio, TX, USA., Rourke E; Department of Urology, UT Health San Antonio Long School of Medicine, San Antonio, TX, USA., Sherrill A; Department of Urology, UT Health San Antonio Long School of Medicine, San Antonio, TX, USA., Johnson-Pais T; Department of Urology, UT Health San Antonio Long School of Medicine, San Antonio, TX, USA.; Department of Cell Systems and Anatomy, UT Health San Antonio Long School of Medicine, San Antonio, TX, USA., Lai Z; Genome Sequencing Facility (GSF) in the Greehey Children's Cancer Research Institute (Greehey CCRI), San Antonio, TX, USA., Basler J; Department of Urology, UT Health San Antonio Long School of Medicine, San Antonio, TX, USA., White JR; Resphera Biosciences, San Antonio, TX, USA., Patterson JE; Department of Medicine, UT Health San Antonio Long School of Medicine, San Antonio, TX, USA. |
| المصدر: | Investigative and clinical urology [Investig Clin Urol] 2019 Mar; Vol. 60 (2), pp. 75-83. Date of Electronic Publication: 2019 Feb 27. |
| نوع المنشور: | Journal Article; Observational Study; Research Support, N.I.H., Extramural |
| اللغة: | English |
| بيانات الدورية: | Publisher: Korean Urological Association Country of Publication: Korea (South) NLM ID: 101674989 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 2466-054X (Electronic) Linking ISSN: 24660493 NLM ISO Abbreviation: Investig Clin Urol Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Seoul : Korean Urological Association, [2016]- |
| مواضيع طبية MeSH: | Gastrointestinal Microbiome*, Escherichia coli/*drug effects , Escherichia coli/*isolation & purification , Fluoroquinolones/*pharmacology , Rectum/*microbiology, Aged ; Carrier State ; Drug Resistance, Bacterial ; Escherichia coli Infections/microbiology ; Humans ; Male ; Middle Aged |
| مستخلص: | Purpose: Fluoroquinolone-resistant (FQR) Escherichia coli causes transrectal prostate biopsy infections. In order to reduce colonization of these bacteria in carriers, we would like to understand the surrounding microbiome to determine targets for decolonization. Materials and Methods: We perform an observational study to investigate the microbiome differences in men with and without FQR organisms found on rectal culture. A rectal swab with two culturettes was performed on men before an upcoming prostate biopsy procedure as standard of care to perform "targeted prophylaxis." Detection of FQR was performed by the standard microbiology lab inoculates the swab onto MacConkey agar containing ciprofloxacin. The extra swab was sent for 16S rRNA amplicon sequencing (MiSeq paired-end) using the V1V2 primer. Alpha and beta-diversity analysis were performed using QIIME. We used PERMANOVA to evaluate the statistical significance of beta-diversity distances within and between groups of interest. Results: We collected 116 rectal swab samples before biopsy for 16S rRNA amplicon sequencing. We identified 18 isolates (15.5%, 18/116) that were positive and had relative reduced diversity profiles (p<0.05). Enterobacteriaceae were significantly over-represented in the FQR subjects (adjusted p=0.03). Conclusions: Microbiome analysis determined that men colonized with FQR bacteria have less diverse bacterial communities (dysbiosis), higher levels of Enterobacteriaceae and reduced levels of Prevotella disiens . These results may have implications in pre/probiotic intervention studies. Competing Interests: CONFLICTS OF INTEREST: Resphera Biosciences was paid to perform the microbiome analysis, and Dr. White is the owner of the company. |
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| معلومات مُعتمدة: | UM1 AI104681 United States AI NIAID NIH HHS |
| فهرسة مساهمة: | Keywords: Anti-bacterial agents; Escherichia coli; Infection; Microbiota; Prostate |
| المشرفين على المادة: | 0 (Fluoroquinolones) |
| تواريخ الأحداث: | Date Created: 20190307 Date Completed: 20200109 Latest Revision: 20200309 |
| رمز التحديث: | 20240829 |
| مُعرف محوري في PubMed: | PMC6397931 |
| DOI: | 10.4111/icu.2019.60.2.75 |
| PMID: | 30838339 |
| قاعدة البيانات: | MEDLINE |
Full Text Finder | تدمد: | 2466-054X |
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| DOI: | 10.4111/icu.2019.60.2.75 |