دورية أكاديمية
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Chemical Characterization and DNA Fingerprinting of Griffonia simplicifolia Baill.

التفاصيل البيبلوغرافية
العنوان: Chemical Characterization and DNA Fingerprinting of Griffonia simplicifolia Baill.
المؤلفون: Vigliante I; Plant Physiology Unit, Department of Life Sciences and Systems Biology, University of Turin, Via Quarello 15/a, 10135 Turin, Italy. ivano.vigliante@unito.it., Mannino G; Plant Physiology Unit, Department of Life Sciences and Systems Biology, University of Turin, Via Quarello 15/a, 10135 Turin, Italy. giuseppe.mannino@unito.it., Maffei ME; Plant Physiology Unit, Department of Life Sciences and Systems Biology, University of Turin, Via Quarello 15/a, 10135 Turin, Italy. massimo.maffei@unito.it.
المصدر: Molecules (Basel, Switzerland) [Molecules] 2019 Mar 15; Vol. 24 (6). Date of Electronic Publication: 2019 Mar 15.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI Country of Publication: Switzerland NLM ID: 100964009 Publication Model: Electronic Cited Medium: Internet ISSN: 1420-3049 (Electronic) Linking ISSN: 14203049 NLM ISO Abbreviation: Molecules Subsets: MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI, c1995-
مواضيع طبية MeSH: DNA Fingerprinting/*methods , Griffonia/*chemistry , Griffonia/*genetics , Medicine, Traditional/*standards, 5-Hydroxytryptophan/chemistry ; Carbolines/chemistry ; Fatty Acids/chemistry ; Molecular Structure ; Plant Extracts/analysis ; Plant Extracts/chemistry ; Polymorphism, Restriction Fragment Length ; Seeds/chemistry ; Seeds/genetics ; Sequence Analysis, DNA/methods
مستخلص: Background: Griffonia simplicifolia Baill. (Caesalpiniaceae) is a medicinal plant whose seeds are widely used in traditional medicine for their high content of 5-hydroxy-l-tryptophan (5-HTP), a direct precursor and enhancer of the activity of the brain hormone serotonin (5-HT). The plant extracts are used in dietary supplements aimed to alleviate serotonin-related disorders.
Methods: In order to characterize the chemical components of G. simplicifolia seeds and their identity, we used a combined methodology by using HPLC-DAD-ESI-MS/MS for the qualitative and quantitative determination of the N -containing compounds, GC-FID and GC-MS for the characterization of the major fatty acids, and DNA fingerprinting based on PCR⁻RFLP for the unequivocal identification of the plant.
Results: 5-HTP was the most representative compound, followed by lower percentages of the β-carboline alkaloid derivative griffonine and other alkaloids. Fatty acids were dominated by the unsaturated fatty acids linoleic acid and oleic acid, followed by the saturated fatty acids stearic and palmitic acids. PCR analysis of the internal transcribed spacer amplified sequence showed a major band at about 758 bp, whereas the PCR⁻RFLP analysis of this sequence using three different restriction enzymes ( MspI , HhaI , and HaeIII ) generated a specific fingerprinting useful for the plant identification.
Conclusions: The combined chemical and molecular analysis of G. simplicifolia provided an interesting integrated approach for the unequivocal identification of commercial G. simplicifolia seeds.
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معلومات مُعتمدة: Local grants Università degli Studi di Torino
فهرسة مساهمة: Keywords: 5-hydroxy-l-tryptophan (5-HTP); Caesalpiniaceae; DNA fingerprinting; Griffonia simplicifolia; PCR–RFLP; fatty acids; internal transcribed spacer (ITS)
المشرفين على المادة: 0 (Carbolines)
0 (Fatty Acids)
0 (Plant Extracts)
94HMA1I78O (norharman)
C1LJO185Q9 (5-Hydroxytryptophan)
تواريخ الأحداث: Date Created: 20190317 Date Completed: 20190701 Latest Revision: 20200225
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC6472197
DOI: 10.3390/molecules24061032
PMID: 30875930
قاعدة البيانات: MEDLINE
الوصف
تدمد:1420-3049
DOI:10.3390/molecules24061032