دورية أكاديمية
أعرض في EDS

Characteristics and homogeneity of N6-methylation in human genomes.

التفاصيل البيبلوغرافية
العنوان: Characteristics and homogeneity of N6-methylation in human genomes.
المؤلفون: Pacini CE; Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, CB2 1QN, UK.; Department of Zoology, University of Cambridge, Cambridge, CB3 3EJ, UK., Bradshaw CR; Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, CB2 1QN, UK., Garrett NJ; Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, CB2 1QN, UK.; Department of Zoology, University of Cambridge, Cambridge, CB3 3EJ, UK., Koziol MJ; Wellcome Trust Cancer Research UK Gurdon Institute, University of Cambridge, Cambridge, CB2 1QN, UK. mjk39@cam.ac.uk.; Department of Zoology, University of Cambridge, Cambridge, CB3 3EJ, UK. mjk39@cam.ac.uk.
المصدر: Scientific reports [Sci Rep] 2019 Mar 26; Vol. 9 (1), pp. 5185. Date of Electronic Publication: 2019 Mar 26.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Nature Publishing Group Country of Publication: England NLM ID: 101563288 Publication Model: Electronic Cited Medium: Internet ISSN: 2045-2322 (Electronic) Linking ISSN: 20452322 NLM ISO Abbreviation: Sci Rep Subsets: MEDLINE
أسماء مطبوعة: Original Publication: London : Nature Publishing Group, copyright 2011-
مواضيع طبية MeSH: Genome, Human*, DNA Methylation/*genetics , Deoxyadenosines/*metabolism, Cell Line ; Chromosomes, Human/metabolism ; Humans ; Transcription, Genetic
مستخلص: A novel DNA modification, N-6 methylated deoxyadenosine (m6dA), has recently been discovered in eukaryotic genomes. Despite its low abundance in eukaryotes, m6dA is implicated in human diseases such as cancer. It is therefore important to precisely identify and characterize m6dA in the human genome. Here, we identify m6dA sites at nucleotide level, in different human cells, genome wide. We compare m6dA features between distinct human cells and identify m6dA characteristics in human genomes. Our data demonstrates for the first time that despite low m6dA abundance, the m6dA mark does often occur consistently at the same genomic location within a given human cell type, demonstrating m6dA homogeneity. We further show, for the first time, higher levels of m6dA homogeneity within one chromosome. Most m6dA are found on a single chromosome from a diploid sample, suggesting inheritance. Our transcriptome analysis not only indicates that human genes with m6dA are associated with higher RNA transcript levels but identifies allele-specific gene transcripts showing haplotype-specific m6dA methylation, which are implicated in different biological functions. Our analyses demonstrate the precision and consistency by which the m6dA mark occurs within the human genome, suggesting that m6dA marks are precisely inherited in humans.
References: Cell. 1994 May 6;77(3):413-26. (PMID: 8011018)
Mamm Genome. 1991;1(2):78-83. (PMID: 1799791)
Cell. 2016 Dec 15;167(7):1897. (PMID: 27984735)
Cell. 1994 Feb 11;76(3):577-88. (PMID: 7906204)
Science. 2017 Apr 21;356(6335):. (PMID: 28428369)
Genome Biol. 2017 Jan 24;18(1):15. (PMID: 28118844)
Proc Natl Acad Sci U S A. 2007 Aug 7;104(32):12955-61. (PMID: 17670940)
Nat Biotechnol. 2012 Dec;30(12):1232-9. (PMID: 23138224)
Nat Genet. 2017 Jun;49(6):964-968. (PMID: 28481340)
Biochem Biophys Res Commun. 2016 Nov 4;480(1):120-125. (PMID: 27693785)
Nat Commun. 2016 Sep 08;7:12817. (PMID: 27605262)
Science. 2009 Jan 2;323(5910):133-8. (PMID: 19023044)
Nat Rev Genet. 2002 Jun;3(6):415-28. (PMID: 12042769)
Development. 2014 Jun;141(11):2173-81. (PMID: 24866112)
Genome Res. 2013 Jan;23(1):129-41. (PMID: 23093720)
EMBO J. 1986 Aug;5(8):2009-13. (PMID: 3019677)
Cancer Res. 2010 Aug 15;70(16):6456-66. (PMID: 20682797)
Cell. 1991 Mar 22;64(6):1123-34. (PMID: 2004419)
Annu Rev Genet. 2008;42:733-72. (PMID: 18729722)
Nature. 1981 Aug 27;292(5826):860-2. (PMID: 6267477)
Mol Cell. 2018 Jul 19;71(2):306-318.e7. (PMID: 30017583)
Cell. 2015 May 7;161(4):879-892. (PMID: 25936837)
Am J Hum Genet. 2007 May;80(5):994-1001. (PMID: 17436255)
Genomics. 2001 Apr 15;73(2):232-7. (PMID: 11318613)
Dev Cell. 2018 May 7;45(3):406-416.e3. (PMID: 29656930)
Nat Commun. 2017 Oct 24;8(1):1122. (PMID: 29066820)
Cell Stem Cell. 2008 Nov 6;3(5):480-3. (PMID: 18983963)
Biochimie. 2018 Jan;144:56-62. (PMID: 29074394)
Cell. 1985 Nov;43(1):117-30. (PMID: 3000598)
Nature. 2016 Oct 13;538(7624):243-247. (PMID: 27706134)
Eur J Protistol. 2017 Apr;58:94-102. (PMID: 28135687)
Mol Cell. 2015 Jun 4;58(5):886-99. (PMID: 26004229)
J Bacteriol. 1953 Feb;65(2):113-21. (PMID: 13034700)
Sci Data. 2014 Nov 25;1:140045. (PMID: 25977796)
Nature. 2009 Aug 20;460(7258):1011-5. (PMID: 19587683)
J Bacteriol. 1952 Oct;64(4):557-69. (PMID: 12999684)
Nat Genet. 2003 Mar;33 Suppl:245-54. (PMID: 12610534)
Nat Rev Genet. 2013 Sep;14(9):609-17. (PMID: 23917626)
Nature. 2012 Apr 29;485(7397):201-6. (PMID: 22575960)
Nat Commun. 2018 Feb 22;9(1):781. (PMID: 29472610)
J Biol Chem. 1948 Aug;175(1):315-32. (PMID: 18873306)
Genome Res. 2014 Dec;24(12):2066-76. (PMID: 25373144)
Nucleic Acids Res. 2012 Dec;40(22):11450-62. (PMID: 23034806)
Nat Rev Genet. 2001 Jan;2(1):21-32. (PMID: 11253064)
Nature. 2016 Apr 21;532(7599):329-33. (PMID: 27027282)
Cell. 2015 May 7;161(4):893-906. (PMID: 25936838)
Genome Res. 2018 Jul;28(7):1067-1078. (PMID: 29764913)
Sci Data. 2016 Jun 07;3:160025. (PMID: 27271295)
Nat Struct Mol Biol. 2016 Jan;23(1):24-30. (PMID: 26689968)
Nat Commun. 2016 Oct 07;7:13052. (PMID: 27713410)
Cell. 2015 May 7;161(4):868-78. (PMID: 25936839)
Nat Methods. 2010 Jun;7(6):461-5. (PMID: 20453866)
Bio Protoc. 2016 Nov 5;6(21):. (PMID: 28180135)
Nat Rev Genet. 2011 Jul 18;12(8):565-75. (PMID: 21765458)
Genomics Proteomics Bioinformatics. 2015 Oct;13(5):278-89. (PMID: 26542840)
Development. 2008 Mar;135(5):909-18. (PMID: 18263842)
Cell. 2018 Nov 15;175(5):1228-1243.e20. (PMID: 30392959)
Cell Stem Cell. 2015 Oct 1;17(4):471-85. (PMID: 26431182)
معلومات مُعتمدة: C6946/A24843 United Kingdom CRUK_ Cancer Research UK; 101050/Z/13/Z United Kingdom WT_ Wellcome Trust; 203144/Z/16/Z United Kingdom WT_ Wellcome Trust; BB/M022994/1 United Kingdom BB_ Biotechnology and Biological Sciences Research Council; United Kingdom WT_ Wellcome Trust
المشرفين على المادة: 0 (Deoxyadenosines)
P582C98ULC (2'-deoxyadenosine)
تواريخ الأحداث: Date Created: 20190328 Date Completed: 20200930 Latest Revision: 20240216
رمز التحديث: 20240216
مُعرف محوري في PubMed: PMC6435722
DOI: 10.1038/s41598-019-41601-7
PMID: 30914725
قاعدة البيانات: MEDLINE
الوصف
تدمد:2045-2322
DOI:10.1038/s41598-019-41601-7