دورية أكاديمية
أعرض في EDS

Curcumin and o-Vanillin Exhibit Evidence of Senolytic Activity in Human IVD Cells In Vitro.

التفاصيل البيبلوغرافية
العنوان: Curcumin and o-Vanillin Exhibit Evidence of Senolytic Activity in Human IVD Cells In Vitro.
المؤلفون: Cherif H; Orthopaedic Research Lab, Department of Surgery, McGill University, 1650 Cedar Ave., C10-148.8, Montreal, QC H3G 1A4, Canada. hosni.cherif@hotmail.com.; McGill Scoliosis and Spine Group, Department of Surgery, McGill University, 1650 Cedar Ave., C10-148.8, Montreal, QC H3G 1A4, Canada. hosni.cherif@hotmail.com.; Department of Surgery, The Research Institute of the McGill University Health Centre, 1650 Cedar Ave., C10-148.8, Montreal, QC H3G 1A4, Canada. hosni.cherif@hotmail.com., Bisson DG; Orthopaedic Research Lab, Department of Surgery, McGill University, 1650 Cedar Ave., C10-148.8, Montreal, QC H3G 1A4, Canada. daniel.g.bisson@gmail.com.; McGill Scoliosis and Spine Group, Department of Surgery, McGill University, 1650 Cedar Ave., C10-148.8, Montreal, QC H3G 1A4, Canada. daniel.g.bisson@gmail.com.; Department of Surgery, The Research Institute of the McGill University Health Centre, 1650 Cedar Ave., C10-148.8, Montreal, QC H3G 1A4, Canada. daniel.g.bisson@gmail.com., Jarzem P; McGill Scoliosis and Spine Group, Department of Surgery, McGill University, 1650 Cedar Ave., C10-148.8, Montreal, QC H3G 1A4, Canada. pjarzem@gmail.com.; Department of Surgery, The Research Institute of the McGill University Health Centre, 1650 Cedar Ave., C10-148.8, Montreal, QC H3G 1A4, Canada. pjarzem@gmail.com., Weber M; McGill Scoliosis and Spine Group, Department of Surgery, McGill University, 1650 Cedar Ave., C10-148.8, Montreal, QC H3G 1A4, Canada. micheal.weber@hotmail.com.; Department of Surgery, The Research Institute of the McGill University Health Centre, 1650 Cedar Ave., C10-148.8, Montreal, QC H3G 1A4, Canada. micheal.weber@hotmail.com., Ouellet JA; McGill Scoliosis and Spine Group, Department of Surgery, McGill University, 1650 Cedar Ave., C10-148.8, Montreal, QC H3G 1A4, Canada. jaouellet@hotmail.com.; Department of Surgery, The Research Institute of the McGill University Health Centre, 1650 Cedar Ave., C10-148.8, Montreal, QC H3G 1A4, Canada. jaouellet@hotmail.com.; Shriner's Hospital for Children, 1003 Decarie Blvd, Montreal, QC H4A 0A9, Canada. jaouellet@hotmail.com., Haglund L; Orthopaedic Research Lab, Department of Surgery, McGill University, 1650 Cedar Ave., C10-148.8, Montreal, QC H3G 1A4, Canada. lisbet.haglund@mcgill.ca.; McGill Scoliosis and Spine Group, Department of Surgery, McGill University, 1650 Cedar Ave., C10-148.8, Montreal, QC H3G 1A4, Canada. lisbet.haglund@mcgill.ca.; Department of Surgery, The Research Institute of the McGill University Health Centre, 1650 Cedar Ave., C10-148.8, Montreal, QC H3G 1A4, Canada. lisbet.haglund@mcgill.ca.; Shriner's Hospital for Children, 1003 Decarie Blvd, Montreal, QC H4A 0A9, Canada. lisbet.haglund@mcgill.ca.
المصدر: Journal of clinical medicine [J Clin Med] 2019 Mar 29; Vol. 8 (4). Date of Electronic Publication: 2019 Mar 29.
نوع المنشور: Journal Article
اللغة: English
بيانات الدورية: Publisher: MDPI AG Country of Publication: Switzerland NLM ID: 101606588 Publication Model: Electronic Cited Medium: Print ISSN: 2077-0383 (Print) Linking ISSN: 20770383 NLM ISO Abbreviation: J Clin Med Subsets: PubMed not MEDLINE
أسماء مطبوعة: Original Publication: Basel, Switzerland : MDPI AG, [2012]-
مستخلص: Curcumin and o-Vanillin cleared senescent intervertebral disc (IVD) cells and reduced the senescence-associated secretory phenotype (SASP) associated with inflammation and back pain. Cells from degenerate and non-mildly-degenerate human IVD were obtained from organ donors and from patients undergoing surgery for low back pain. Gene expression of senescence and SASP markers was evaluated by RT-qPCR in isolated cells, and protein expression of senescence, proliferation, and apoptotic markers was evaluated by immunocytochemistry (ICC). The expression levels of SASP factors were evaluated by enzyme-linked immunosorbent assay (ELISA). Matrix synthesis was verified with safranin-O staining and the Dimethyl-Methylene Blue Assay for proteoglycan content. Western blotting and ICC were used to determine the molecular pathways targeted by the drugs. We found a 40% higher level of senescent cells in degenerate compared to non-mildly-degenerate discs from unrelated individuals and a 10% higher level in degenerate compared to non-mildly-degenerate discs from the same individual. Higher levels of senescence were associated with increased SASP. Both drugs cleared senescent cells, and treatment increased the number of proliferating as well as apoptotic cells in cultures from degenerate IVDs. The expression of SASP factors was decreased, and matrix synthesis increased following treatment. These effects were mediated through the Nrf2 and NFkB pathways.
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معلومات مُعتمدة: CIHR MOP-119564 Canada Canadian Institutes of Health Research; 2018 Réseau de Recherche en Santé Buccodentaire et Osseuse
فهرسة مساهمة: Keywords: back pain; degeneration; inflammation; intervertebral disc; senescence; senolytics
تواريخ الأحداث: Date Created: 20190403 Latest Revision: 20201001
رمز التحديث: 20231215
مُعرف محوري في PubMed: PMC6518239
DOI: 10.3390/jcm8040433
PMID: 30934902
قاعدة البيانات: MEDLINE
الوصف
تدمد:2077-0383
DOI:10.3390/jcm8040433