دورية أكاديمية
Biocompatibility and immunogenic response to recombinant honeybee silk material.
| العنوان: | Biocompatibility and immunogenic response to recombinant honeybee silk material. |
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| المؤلفون: | Sutherland TD; CSIRO, Black Mountain, GPO Box 1700, Acton, Canberra, Australian Capital Territory, 2601, Australia., Vashi AV; CSIRO, Black Mountain, GPO Box 1700, Acton, Canberra, Australian Capital Territory, 2601, Australia., Kardia E; CSIRO, Black Mountain, GPO Box 1700, Acton, Canberra, Australian Capital Territory, 2601, Australia., Sriskantha A; CSIRO, Black Mountain, GPO Box 1700, Acton, Canberra, Australian Capital Territory, 2601, Australia., Rapson TD; CSIRO, Black Mountain, GPO Box 1700, Acton, Canberra, Australian Capital Territory, 2601, Australia., Hall RN; CSIRO, Black Mountain, GPO Box 1700, Acton, Canberra, Australian Capital Territory, 2601, Australia., Werkmeister JA; CSIRO, Black Mountain, GPO Box 1700, Acton, Canberra, Australian Capital Territory, 2601, Australia. |
| المصدر: | Journal of biomedical materials research. Part A [J Biomed Mater Res A] 2019 Aug; Vol. 107 (8), pp. 1763-1770. Date of Electronic Publication: 2019 May 01. |
| نوع المنشور: | Journal Article; Research Support, Non-U.S. Gov't |
| اللغة: | English |
| بيانات الدورية: | Publisher: John Wiley & Sons Country of Publication: United States NLM ID: 101234237 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1552-4965 (Electronic) Linking ISSN: 15493296 NLM ISO Abbreviation: J Biomed Mater Res A Subsets: MEDLINE |
| أسماء مطبوعة: | Original Publication: Hoboken, NJ : John Wiley & Sons, c2002- |
| مواضيع طبية MeSH: | Bees/*chemistry , Biocompatible Materials/*pharmacology , Recombinant Proteins/*immunology , Silk/*immunology, Animals ; Cell Death/drug effects ; Cell Line ; Cell Survival/drug effects ; Disease Models, Animal ; Female ; Inflammation/pathology ; Mice ; Prosthesis Implantation ; Rats, Sprague-Dawley ; Subcutaneous Tissue/drug effects ; Time Factors |
| مستخلص: | If tolerated in biological environments, recombinant structural proteins offer the advantage that biological cues dictating cell attachment and material degradation can be modified as required for clinical application using genetic engineering. In this study, we investigate the biological response to materials generated from the recombinant honeybee silk protein, AmelF3, a structural protein that can be produced at high levels by fermentation in Escherichia coli. The protein can be readily purified from E. coli host cell proteins after transgenic production and fabricated into various material formats. When implanted subcutaneously according to International Standard ISO 10993 tests, materials generated from the purified recombinant protein were found to be noncytotoxic, inducing a transient weak immunogenic response and a chronic inflammatory response that resolved over time. While preliminary, this study supports the ongoing development of materials generated from this protein for biomedical applications. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1763-1770, 2019. (© 2019 Wiley Periodicals, Inc.) |
| معلومات مُعتمدة: | International Commonwealth Scientific and Industrial Research Organisation |
| فهرسة مساهمة: | Keywords: IgG; biomaterial; cytotoxic; immune properties; recombinant protein |
| المشرفين على المادة: | 0 (Biocompatible Materials) 0 (Recombinant Proteins) 0 (Silk) |
| تواريخ الأحداث: | Date Created: 20190416 Date Completed: 20200812 Latest Revision: 20200812 |
| رمز التحديث: | 20240628 |
| DOI: | 10.1002/jbm.a.36695 |
| PMID: | 30983124 |
| قاعدة البيانات: | MEDLINE |
| تدمد: | 1552-4965 |
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| DOI: | 10.1002/jbm.a.36695 |