دورية أكاديمية
Comparison of histochemical staining techniques for detecting mast cells in oral lesions.
| العنوان: | Comparison of histochemical staining techniques for detecting mast cells in oral lesions. |
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| المؤلفون: | Mutsaddi S; Department of Oral Pathology and Microbiology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre , Belgaum , India., Kotrashetti VS; Department of Oral Pathology and Microbiology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre , Belgaum , India., Nayak RS; Department of Oral Pathology and Microbiology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre , Belgaum , India., Pattanshetty SM; Department of Oral Pathology and Microbiology, Maratha Mandal's NGH Institute of Dental Sciences and Research Centre , Belgaum , India. |
| المصدر: | Biotechnic & histochemistry : official publication of the Biological Stain Commission [Biotech Histochem] 2019 Aug; Vol. 94 (6), pp. 459-468. Date of Electronic Publication: 2019 Apr 15. |
| نوع المنشور: | Comparative Study; Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Taylor & Francis Country of Publication: England NLM ID: 9107378 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1473-7760 (Electronic) Linking ISSN: 10520295 NLM ISO Abbreviation: Biotech Histochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: 2015- : Abingdon, Oxford : Taylor & Francis Original Publication: Baltimore; MD : Williams & Wilkins, c1991- |
| مواضيع طبية MeSH: | Carcinoma, Squamous Cell/*pathology , Mast Cells/*pathology , Mouth Neoplasms/*pathology, Alcian Blue/pharmacology ; Cell Count/methods ; Coloring Agents/pharmacology ; Histological Techniques/methods ; Humans ; Staining and Labeling/methods ; Tolonium Chloride/pharmacology |
| مستخلص: | Mast cells are large cells with granular cytoplasm that participate in wound healing, angiogenesis and defense against pathogens. They also contribute to inflammation by initiating innate and acquired immunity. The granules of these cells exhibit characteristic staining properties. We investigated toluidine blue, astra blue, Alcian blue-pyronin Y and May-Grunwald Giemsa stains for mast cells in various oral lesions and assessed the efficacy of each for identifying mast cells. Sections were obtained from 10 each of diagnosed cases of inflammatory fibrous hyperplasia, periapical cyst, mild dysplasia, oral submucous fibrosis and oral squamous cell carcinoma and stained using the stains listed above. Mast cells were assessed for their presence, contrast of the mast cell in the connective tissue background and number. We found that May-Grunwald Giemsa stain was the best for identification of mast cells, although toluidine blue staining is less time-consuming. Overall we obtained better results using May-Grunwald Giemsa and toluidine blue for staining mast cells. |
| فهرسة مساهمة: | Keywords: Alcian blue; Giemsa; May-Grunwald; astra blue; eosin; hematoxylin; mast cells; metachromatic stains; pyronin Y; toluidine blue |
| المشرفين على المادة: | 0 (Coloring Agents) 15XUH0X66N (Tolonium Chloride) P4448TJR7J (Alcian Blue) |
| تواريخ الأحداث: | Date Created: 20190416 Date Completed: 20200218 Latest Revision: 20200218 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1080/10520295.2019.1597986 |
| PMID: | 30983422 |
| قاعدة البيانات: | MEDLINE |
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Full Text Finder | تدمد: | 1473-7760 |
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| DOI: | 10.1080/10520295.2019.1597986 |