دورية أكاديمية
A Transcriptomic Insight into the Impact of Colon Cancer Cells on Mast Cells.
| العنوان: | A Transcriptomic Insight into the Impact of Colon Cancer Cells on Mast Cells. |
|---|---|
| المؤلفون: | Yu Y; Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3584CG Utrecht, The Netherlands. y.yu@uu.nl., Blokhuis BR; Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3584CG Utrecht, The Netherlands. b.r.j.blokhuis@uu.nl., Garssen J; Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3584CG Utrecht, The Netherlands. j.garssen@uu.nl.; Nutricia Research, 3584CT Utrecht, The Netherlands. j.garssen@uu.nl., Redegeld FA; Division of Pharmacology, Utrecht Institute for Pharmaceutical Sciences, Faculty of Science, Utrecht University, 3584CG Utrecht, The Netherlands. F.A.M.Redegeld@uu.nl. |
| المصدر: | International journal of molecular sciences [Int J Mol Sci] 2019 Apr 04; Vol. 20 (7). Date of Electronic Publication: 2019 Apr 04. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: MDPI Country of Publication: Switzerland NLM ID: 101092791 Publication Model: Electronic Cited Medium: Internet ISSN: 1422-0067 (Electronic) Linking ISSN: 14220067 NLM ISO Abbreviation: Int J Mol Sci |
| أسماء مطبوعة: | Original Publication: Basel, Switzerland : MDPI, [2000- |
| مواضيع طبية MeSH: | Colonic Neoplasms/*metabolism , Mast Cells/*metabolism , Transcriptome/*genetics, Cells, Cultured ; Colonic Neoplasms/genetics ; Cytokines/metabolism ; HT29 Cells ; Humans ; Matrix Metalloproteinase 2/metabolism ; Platelet-Derived Growth Factor/metabolism ; Receptor, Notch1/metabolism ; Ubiquitins/metabolism ; Vascular Endothelial Growth Factor A/metabolism |
| مستخلص: | Mast cells (MCs) are one of the first immune cells recruited to a tumor. It is well recognized that MCs accumulate in colon cancer lesion and their density is associated with the clinical outcomes. However, the molecular mechanism of how colon cancer cells may modify MC function is still unclear. In this study, primary human MCs were generated from CD34⁺ progenitor cells and a 3D coculture model was developed to study the interplay between colon cancer cells and MCs. By comparing the transcriptomic profile of colon cancer-cocultured MCs versus control MCs, we identified a number of deregulated genes, such as MMP-2, VEGF-A, PDGF-A, COX2, NOTCH1 and ISG15, which contribute to the enrichment of cancer-related pathways. Intriguingly, pre-stimulation with a TLR2 agonist prior to colon cancer coculture induced upregulation of multiple interferon-inducible genes as well as MHC molecules in MCs. Our study provides an alternative approach to study the influence of colon cancer on MCs. The transcriptome signature of colon cancer-cocultured MCs may potentially reflect the mechanism of how colon cancer cells educate MCs to become pro-tumorigenic in the initial phase and how a subsequent inflammatory signal-e.g., TLR2 ligands-may modify their responses in the cancer milieu. |
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| معلومات مُعتمدة: | 201408440270 Chinese research council |
| فهرسة مساهمة: | Keywords: 3D coculture; colon cancer; mast cells; transcriptome |
| المشرفين على المادة: | 0 (Cytokines) 0 (Platelet-Derived Growth Factor) 0 (Receptor, Notch1) 0 (Ubiquitins) 0 (Vascular Endothelial Growth Factor A) 0 (platelet-derived growth factor A) 60267-61-0 (ISG15 protein, human) EC 3.4.24.24 (Matrix Metalloproteinase 2) |
| تواريخ الأحداث: | Date Created: 20190417 Date Completed: 20190805 Latest Revision: 20200225 |
| رمز التحديث: | 20221213 |
| مُعرف محوري في PubMed: | PMC6480031 |
| DOI: | 10.3390/ijms20071689 |
| PMID: | 30987352 |
| قاعدة البيانات: | MEDLINE |
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