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Effect of a pneumococcal whole cell vaccine on influenza A-induced pneumococcal otitis media in infant mice.

التفاصيل البيبلوغرافية
العنوان: Effect of a pneumococcal whole cell vaccine on influenza A-induced pneumococcal otitis media in infant mice.
المؤلفون: Manning J; Pneumococcal Research, Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia; Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia., Dunne EM; Pneumococcal Research, Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia; Department of Paediatrics, Royal Children's Hospital, The University of Melbourne, Melbourne, Victoria, Australia., Wang N; Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia., Pedersen JS; TissuPath Pty. Ltd., Hawthorn, Victoria, Australia., Ogier JM; Department of Paediatrics, Royal Children's Hospital, The University of Melbourne, Melbourne, Victoria, Australia; Neurogenetics, Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia., Burt RA; Department of Paediatrics, Royal Children's Hospital, The University of Melbourne, Melbourne, Victoria, Australia; Neurogenetics, Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia., Mulholland EK; Pneumococcal Research, Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia; Department of Infectious Disease Epidemiology, London School of Hygiene & Tropical Medicine, London, United Kingdom., Robins-Browne RM; Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia; Infectious Diseases, Murdoch Children's Research Institute, Parkville, VIC, Australia., Malley R; Division of Infectious Diseases, Boston Children's Hospital, Boston, United States., Wijburg OL; Pneumococcal Research, Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia; Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia., Satzke C; Pneumococcal Research, Murdoch Children's Research Institute, Royal Children's Hospital, Parkville, Victoria, Australia; Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, Melbourne, Victoria, Australia; Department of Paediatrics, Royal Children's Hospital, The University of Melbourne, Melbourne, Victoria, Australia. Electronic address: catherine.satzke@mcri.edu.au.
المصدر: Vaccine [Vaccine] 2019 Jun 06; Vol. 37 (26), pp. 3495-3504. Date of Electronic Publication: 2019 May 15.
نوع المنشور: Journal Article; Research Support, Non-U.S. Gov't
اللغة: English
بيانات الدورية: Publisher: Elsevier Science Country of Publication: Netherlands NLM ID: 8406899 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1873-2518 (Electronic) Linking ISSN: 0264410X NLM ISO Abbreviation: Vaccine Subsets: MEDLINE
أسماء مطبوعة: Publication: Amsterdam, The Netherlands : Elsevier Science
Original Publication: [Guildford, Surrey, UK] : Butterworths, [c1983-
مواضيع طبية MeSH: Influenza A Virus, H3N2 Subtype/*immunology , Orthomyxoviridae Infections/*immunology , Otitis Media/*immunology , Pneumococcal Infections/*immunology , Pneumococcal Vaccines/*immunology, Adjuvants, Immunologic/administration & dosage ; Animals ; Carrier State/immunology ; Disease Models, Animal ; Ear, Middle/immunology ; Mice ; Mice, Inbred C57BL ; Nasopharynx ; Serogroup ; Vaccination/methods ; Vaccines, Conjugate/immunology
مستخلص: The pneumococcus remains a common cause of otitis media (OM) despite the widespread introduction of pneumococcal conjugate vaccines. In mice, a pneumococcal whole cell vaccine (WCV) induces serotype-independent protection against pneumococcal colonisation and invasive disease via T H 17- and antibody-mediated immunity, respectively. We investigated the effect of WCV on influenza A-induced pneumococcal OM in an infant mouse model. C57BL/6 mice were immunised subcutaneously with a single dose of WCV or adjuvant at 6 days of age, infected with pneumococci (EF3030 [serotype 19F] or PMP1106 [16F]) at 12 days of age, and given influenza A virus (A/Udorn/72/307 [H3N2], IAV) at 18 days of age to induce pneumococcal OM. Pneumococcal density in middle ear and nasopharyngeal tissues was determined 6 and 12 days post-virus. Experiments were repeated in antibody (B6.μMT -/- )- and CD4 + T-cell-deficient mice to investigate the immune responses involved. A single dose of WCV did not prevent the development of pneumococcal OM, nor accelerate pneumococcal clearance compared with mice receiving adjuvant alone. However, WCV reduced the density of EF3030 in the middle ear at 6 days post-viral infection (p = 0.022), and the density of both isolates in the nasopharynx at 12 days post-viral infection (EF3030, p = 0.035; PMP1106, p = 0.011), compared with adjuvant alone. The reduction in density in the middle ear required antibodies and CD4 + T cells: WCV did not reduce EF3030 middle ear density in B6.μMT -/- mice (p = 0.35) nor in wild-type mice given anti-CD4 monoclonal antibody before and after IAV inoculation (p = 0.91); and WCV-immunised CD4 + T cell-deficient GK1.5 mice had higher levels of EF3030 in the middle ear than their adjuvant-immunised counterparts (p = 0.044). A single subcutaneous dose of WCV reduced pneumococcal density in the middle ears of co-infected mice in one of two strains tested, but did not prevent OM from occurring in this animal model.
(Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.)
فهرسة مساهمة: Keywords: Influenza A virus; Mouse model; Otitis media; Pneumococcus; Streptococcus pneumoniae; Vaccine; Whole cell vaccine
المشرفين على المادة: 0 (Adjuvants, Immunologic)
0 (Pneumococcal Vaccines)
0 (Vaccines, Conjugate)
تواريخ الأحداث: Date Created: 20190520 Date Completed: 20200908 Latest Revision: 20200908
رمز التحديث: 20231215
DOI: 10.1016/j.vaccine.2019.03.013
PMID: 31103366
قاعدة البيانات: MEDLINE
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