دورية أكاديمية
Chromosomes missegregated into micronuclei contribute to chromosomal instability by missegregating at the next division.
| العنوان: | Chromosomes missegregated into micronuclei contribute to chromosomal instability by missegregating at the next division. |
|---|---|
| المؤلفون: | He B; Department of Biological Sciences, Virginia Tech, Blacksburg, VA 24061, USA.; Biocomplexity Institute, Virginia Tech, Blacksburg, VA 24061, USA., Gnawali N; Department of Biological Sciences, Virginia Tech, Blacksburg, VA 24061, USA.; Current affiliation: Orlando Health, MP 401, Orlando, FL 32819, USA., Hinman AW; Department of Biological Sciences, Virginia Tech, Blacksburg, VA 24061, USA.; Biocomplexity Institute, Virginia Tech, Blacksburg, VA 24061, USA.; Current affiliation: Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305, USA., Mattingly AJ; Department of Biological Sciences, Virginia Tech, Blacksburg, VA 24061, USA.; Current affiliation: Department of Cell and Tissue Biology, UCSF, San Francisco, CA 94122, USA., Osimani A; Biocomplexity Institute, Virginia Tech, Blacksburg, VA 24061, USA., Cimini D; Department of Biological Sciences, Virginia Tech, Blacksburg, VA 24061, USA.; Biocomplexity Institute, Virginia Tech, Blacksburg, VA 24061, USA. |
| المصدر: | Oncotarget [Oncotarget] 2019 Apr 12; Vol. 10 (28), pp. 2660-2674. Date of Electronic Publication: 2019 Apr 12 (Print Publication: 2019). |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Impact Journals Country of Publication: United States NLM ID: 101532965 Publication Model: eCollection Cited Medium: Internet ISSN: 1949-2553 (Electronic) Linking ISSN: 19492553 NLM ISO Abbreviation: Oncotarget Subsets: PubMed not MEDLINE |
| أسماء مطبوعة: | Original Publication: Albany, N.Y. : Impact Journals |
| مستخلص: | Micronuclei (MNi) are extranuclear DNA-containing structures that form upon mitotic exit from unsegregated chromosome fragments or anaphase lagging (whole) chromosomes (LCs). MNi formed from whole chromosomes are of particular interest because LCs are observed in both cancer and non-cancer cells, and are recognized as a major source of chromosomal instability (CIN) in cancer cells. Here, we generated a PtK1 cell line expressing a photoactivatable H2B histone to study the behavior of whole chromosome-containing MNi at the mitosis following their formation. Importantly, MNi of PtK1 cells did not display the membrane rupture or transport defects reported for other cell types. Despite this, we found that most micronucleated cells displayed some kind of chromosome segregation defect and that the missegregating chromosome was the one derived from the MN. Moreover, condensation of the chromosome within the MN was frequently delayed and associated with failure to align at the metaphase plate. Finally, the defective condensation of the MN-derived chromosomes could also explain the frequent occurrence of cytokinesis failure in micronucleated cells. In summary, we find that chromosomes from MNi may trigger a CIN phenotype by missegregating at the mitosis following MN formation. Competing Interests: CONFLICTS OF INTEREST The authors have no conflicts of interest to declare. |
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| فهرسة مساهمة: | Keywords: Chromosome; lagging chromosome; micronucleus; missegregation; mitosis |
| تواريخ الأحداث: | Date Created: 20190521 Latest Revision: 20200225 |
| رمز التحديث: | 20231215 |
| مُعرف محوري في PubMed: | PMC6505630 |
| DOI: | 10.18632/oncotarget.26853 |
| PMID: | 31105868 |
| قاعدة البيانات: | MEDLINE |
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