دورية أكاديمية
Overexpression of miR-4286 is an unfavorable prognostic marker in individuals with non-small cell lung cancer.
| العنوان: | Overexpression of miR-4286 is an unfavorable prognostic marker in individuals with non-small cell lung cancer. |
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| المؤلفون: | An X; Health Care Unit, Jining No.1 People's Hospital, Jining, China., Ge J; Department of Respiratory Medicine, Affiliated Hospital of Jining Medical College, Jining, China., Guo H; Department of Respiratory Medicine, Jining No.1 People's Hospital, Jining, China., Mi H; Department of Cardiac Surgery, Jining No.1 People's Hospital, Jining, China., Zhou J; Department of Respiratory Medicine, Jining No.1 People's Hospital, Jining, China., Liu Y; Department of Respiratory Medicine, Jining No.1 People's Hospital, Jining, China., Weiyue; Department of Respiratory Medicine, Jining No.1 People's Hospital, Jining, China., Wu Z; Health Care Unit, Jining No.1 People's Hospital, Jining, China. |
| المصدر: | Journal of cellular biochemistry [J Cell Biochem] 2019 Oct; Vol. 120 (10), pp. 17573-17583. Date of Electronic Publication: 2019 May 20. |
| نوع المنشور: | Journal Article |
| اللغة: | English |
| بيانات الدورية: | Publisher: Wiley-Liss Country of Publication: United States NLM ID: 8205768 Publication Model: Print-Electronic Cited Medium: Internet ISSN: 1097-4644 (Electronic) Linking ISSN: 07302312 NLM ISO Abbreviation: J Cell Biochem Subsets: MEDLINE |
| أسماء مطبوعة: | Publication: <2004>- : Hoboken, NJ : Wiley-Liss Original Publication: New York : Liss, c1982- |
| مواضيع طبية MeSH: | Prognosis*, Carcinoma, Non-Small-Cell Lung/*genetics , Core Binding Factor Alpha 3 Subunit/*genetics , MicroRNAs/*genetics, A549 Cells ; Adult ; Aged ; Apoptosis/genetics ; Biomarkers, Tumor/genetics ; Carcinoma, Non-Small-Cell Lung/epidemiology ; Carcinoma, Non-Small-Cell Lung/pathology ; Cell Cycle Checkpoints/genetics ; Cell Line, Tumor ; Cell Movement/genetics ; Cell Proliferation/genetics ; Disease-Free Survival ; Female ; Gene Expression Regulation, Neoplastic/genetics ; Humans ; Lymphatic Metastasis ; Male ; Middle Aged ; Neoplasm Invasiveness/genetics ; Neoplasm Invasiveness/pathology |
| مستخلص: | Non-small cell lung cancer (NSCLC) is still an unresolved source of tumor-related death internationally. Current studies have discovered that microRNAs (miRNAs) are associated with diverse cancers development, including NSCLC. Our paper focused on the functional character of miR-4286 in NSCLC. miR-4286 level in 68 cases of NSCLC tissues, matched neighboring nontumor tissues and different cancer cell lines were inspected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). The connection concerning miR-4286 expression and clinicopathological features of patients with NSCLC were further determined. After knockdown or overexpression of miR-4286, cell viability, cell cycle, and/or apoptotic cells were examined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry assay, respectively. Moreover, the cell cycle- and apoptosis-related proteins were estimated by qRT-PCR and Western blot. In comparison with the matched nontumor tissues, miR-4286 was significantly enhanced in lung malignancy tissues and different cell lines. miR-4286 expression was related with the tumor-node-metastasis stage, lymphatic metastasis, and distant metastasis. Cell viability was ominously weakened by suppression of miR-4286 in A549 cells, whereas was statistically upregulated by overexpression of miR-4286 in NCI-H1299 cells. Additionally, we detected that suppression of miR-4286 tempted cell cycle arrest in G1 stage and fortified apoptosis in A549 cells. Runx3 was recognized as one target gene of miR-4286, and the impacts of suppression of miR-4286 on cell viability and apoptosis were through regulation of Runt-related transcription factor 3. Our study suggests that miR-4286 overexpression represents a tumor promoter role in NSCLC cells. (© 2019 Wiley Periodicals, Inc.) |
| فهرسة مساهمة: | Keywords: Runx3; cell apoptosis; cell viability; miR-4286; non-small cell lung cancer |
| المشرفين على المادة: | 0 (Biomarkers, Tumor) 0 (Core Binding Factor Alpha 3 Subunit) 0 (MIRN-4286 microRNA, human) 0 (MicroRNAs) 0 (Runx3 protein, human) |
| تواريخ الأحداث: | Date Created: 20190522 Date Completed: 20200914 Latest Revision: 20200914 |
| رمز التحديث: | 20221213 |
| DOI: | 10.1002/jcb.29024 |
| PMID: | 31111550 |
| قاعدة البيانات: | MEDLINE |
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